Relación entre el estrés y las bacterias entomopatógenas Pantoea (Erwinia) agglomerans (herbicola) y Bacillus cereus en jobotos (Col: Melolonthidae) (Phyllophaga spp., Anomala spp. y Cyclocephala spp.), en Costa Rica.

Abstract

Concentrations of Colony Forming Units (CFU) were determined for two entomopathogenic bacteria (Pantoea agglomerans and Bacillus cereus), at the egg, larval, pupal, and adult stages of agriculturally important Phyllophaga and Cyclocephala white grubs, which were collected in five agroecosystems in Costa Rica. L2 and L3 larvae of Phyllophaga elenans collected in all regions where the study was conducted were extensive carriers of Pantoea agglomerans and Bacillus cereu. L2 and L3 larvae of Phyllophaga obsoleta, Phyllophaga menetriesi, Cyclocephala sanguinicollis and Cyclocephala castaniella found in the Central Valley and Central Pacific regions were carriers of Pantoea agglomerans and Bacillus cereus bacteria. In 60% to 90% of larvae in all white grub varieties studied, Pantoea agglomerans showed greater concentrations of CFU than Bacillus cereu, which showed the lowest CFU concentration. Egg, pupal, and adult mortality in all Phyllophaga species was due to Pantoea agglomerans in 62%, 80% and 22.5% of the cases, respectively. A possible antagonistic interaction between Pantoea agglomerans and Bacillus cereus is also discussed. In general, it was noted that light and larval manipulation were the main stress factors affecting these scarabids.

Annotated catalog and bibliography of the cyclocephaline scarab beetles (Coleoptera, Scarabaeidae, Dynastinae, Cyclocephalini)

“…Bates 1888 , Arrow 1937b , Blackwelder 1944 , Martínez 1964 , Pike et al 1976 , Endrődi 1966 , 1985a , Abarca and Quesada 1997, Vargas and Abarca 1998 , Ratcliffe 1992a , 2002a , 2003 , Krajcik 2005 , 2012 .…”

“… Burmeister 1847 , Reiche 1859 , Harold 1869b , Anonymous 1879 , Bates 1888 , Casey 1915 , Arrow 1937b , Blackwelder 1944 , Pike et al 1976 , Endrődi 1966 , 1967b , 1985a , Morón et al 1985 , Thomas 1993 , Lobo and Morón 1993 , Morón 1979 , 1994 , Ratcliffe and Morón 1997 , Sanchez Soto 1997 , Abarca and Quesada 1997, Vargas and Abarca 1998 , Carrillo-Ruiz and Morón 2003 , Ratcliffe 2003 , Restrepo et al 2003, Ratcliffe and Cave 2006 , Smith 2003 , 2009 , Krajcik 2005 , 2012 , Breeschoten et al 2013 , Ratcliffe et al 2013 , Gasca-Álvarez and Deloya 2016 .…”