Rekonstitution von Kanalproteinen in (polymerisierten) ABA-Triblockcopolymer-Membranen

Meier, Wolfgang; Nardin, Corinne; Winterhalter, Mathias

doi:10.1002/1521-3757(20001215)112:24<4747::aid-ange4747>3.0.co;2-h

Cited by 27 publications

(6 citation statements)

References 19 publications

(22 reference statements)

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“…[113] Außerdem berichteten Naumann et al über den Einfluss der Polymerlinker auf die Phospholipiddiffusion in der Membran: [114] Die Phospholipiddiffusion wurde behindert, was wahrscheinlich auf eine Lipopolymer-induzierte Protrusion der Membran und/oder Clusterbildung des Lipopolymers zurückzuführen ist, die zu einer Membrankrümmung führen. [115][116][117] und Montemagno [118][119][120] über Polymersome aus Poly(2-oxazolin)-block-poly(dimethylsiloxan)-block-poly(2-oxazolin)-Triblockcopolymeren als biomimetische Membranen erwähnt werden.…”

Section: Selbstorganisation Von Endfunktionalisiertenunclassified

Poly(2‐oxazoline): eine Polymerklasse mit vielfältigen Anwendungsmöglichkeiten

Hoogenboom

2009

Angewandte Chemie

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Die lebende kationische Ringöffnungspolymerisation von 2‐Oxazolinen ist seit ihrer Entdeckung 1966 eingehend untersucht worden. Die Vielseitigkeit der lebenden Polymerisation ermöglicht die Copolymerisation verschiedener 2‐Oxazolin‐Monomere zu Polymeren mit einstellbaren Eigenschaften; so lassen sich z. B. hydrophile, hydrophobe, fluorophile sowie harte und weiche Materialien erhalten. Allerdings geriet diese Polymerklasse wegen der langen Reaktionszeiten und ihrer beschränkten Anwendungsmöglichkeiten in den 1980er und 1990er Jahren fast in Vergessenheit. Im neuen Jahrtausend erlebten die Poly(2‐oxazoline) jedoch einen neuen Aufschwung wegen ihrer möglichen Verwendung als Biomaterialien oder thermoresponsive Stoffe sowie dank der Tatsache, dass sie einen leichten Zugang zu definierten amphiphilen Strukturen für die (hierarchische) Selbstorganisation eröffnen. In diesem Aufsatz werden neue Entwicklungen beschrieben, die das Potenzial der Poly(2‐oxazoline) demonstrieren. Außerdem wird die vielversprechende Kombination von Poly(2‐oxazolinen) mit der Klickchemie diskutiert.

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Section: Selbstorganisation Von Endfunktionalisiertenunclassified

Poly(2‐oxazoline): eine Polymerklasse mit vielfältigen Anwendungsmöglichkeiten

Hoogenboom

2009

Angewandte Chemie

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“…We chose PBD 22 ‐PEO 14 (PBD‐PEO 1800) diblock co‐polymer (MW PBD:PEO of 1200:600) as a membrane matrix since it forms 10–12 nm thick polymer bilayers that provide an ideal match for CNTP length. Block‐copolymer membranes were previously used to incorporate a variety of membrane proteins, such as aquaporin Z, ATP synthase, GPCRs, and OmpF porins; therefore, we expected that CNTPs would also incorporate into this membrane ( Figure a). To insert CNTPs into the block‐copolymer membranes, we modified previously developed procedures for inserting CNTPs into lipid matrices, by adding elevated temperatures and constant stirring necessary for PBD‐PEO 1800 to form vesicles.…”

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confidence: 99%

Carbon Nanotube Porins in Amphiphilic Block Copolymers as Fully Synthetic Mimics of Biological Membranes

et al. 2018

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Biological membranes provide a fascinating example of a separation system that is multifunctional, tunable, precise, and efficient. Biomimetic membranes, which mimic the architecture of cellular membranes, have the potential to deliver significant improvements in specificity and permeability. Here, a fully synthetic biomimetic membrane is reported that incorporates ultra-efficient 1.5 nm diameter carbon nanotube porin (CNTPs) channels in a block-copolymer matrix. It is demonstrated that CNTPs maintain high proton and water permeability in these membranes. CNTPs can also mimic the behavior of biological gap junctions by forming bridges between vesicular compartments that allow transport of small molecules. MembranesEnergy-efficient molecular separations are fundamental to a number of modern industrial, environmental, and biomedical processes including large-scale water treatment, water desalination, kidney dialysis, sterile filtration, and manufacturing of pharmaceuticals. [1] Although synthetic polymeric membranes have come to dominate this application landscape, ever increasing demands continue to fuel the search for energyefficient membranes that can provide both high selectivity and high permeability in the critical ca. 1 nm pore size.

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“…Recently, it was reported that synthetic membranes serve as mimics for biological membranes, and were successfully used to incorporate membrane proteins. [14][15][16][17][18] In particular, vesicles resulting from the auto-assembling of poly(2-methyloxazoline)-block-polydimethylsiloxane-block-poly(2-methyloxazoline) copolymer (PMOXA n -PDMS m -PMOXA n ), have been shown to enable functional reconstitution of channel-forming proteins, such as OmpF, LamB, or Aquaporin Z. [19][20][21][22] In addition, amphiphilic copolymer vesicles have been used to design and build nanoreactors, which protect enzymes encapsulated in their inner cavity and enable them to act in situ, [23] or enable the tandem action of soluble and membrane-bound proteins.…”

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confidence: 99%

Amphiphilic Copolymer Membranes Promote NADH:Ubiquinone Oxidoreductase Activity: Towards an Electron‐Transfer Nanodevice

Graff

Fraysse-Ailhas

Palivan

et al. 2010

Macro Chemistry & Physics

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Nanoscale devices for energy conversion require the transfer of electrons from one compartment to another. The enzyme complex I, which in vivo mediates electron transfer from NADH to ubiquinone, is an intriguing candidate for this role in nanodevices. However, complex I normally requires the presence of lipids to remain active, potentially limiting its application. Here we demonstrate for the first time that complex I can be actively reconstituted in the synthetic membrane of amphiphilic triblock copolymer vesicles. The functionality of the reconstituted protein was characterized by EPR and activity assays. Its activity is strongly influenced by the molar mass and the block length of the membrane‐forming polymers, and increases with increasing membrane thickness.magnified image

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Rekonstitution von Kanalproteinen in (polymerisierten) ABA-Triblockcopolymer-Membranen

Cited by 27 publications

References 19 publications

Poly(2‐oxazoline): eine Polymerklasse mit vielfältigen Anwendungsmöglichkeiten

Poly(2‐oxazoline): eine Polymerklasse mit vielfältigen Anwendungsmöglichkeiten

Carbon Nanotube Porins in Amphiphilic Block Copolymers as Fully Synthetic Mimics of Biological Membranes

Amphiphilic Copolymer Membranes Promote NADH:Ubiquinone Oxidoreductase Activity: Towards an Electron‐Transfer Nanodevice

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