“…A comparison between root and shoot saponin mixtures showed that this compound was purified in fractions eluted with 45-56 % iso-propanol and appears to be the major saponin present in both tissues, previously described as having R f 0·43 (16) (result not shown here). (19) gave a dark olive-coloured spot at R f 0·29, but did not appear to be present in the root or shoot saponin mixtures in the present work.…”
Section: Identification Of Alfalfa Saponins Using Reference Compoundscontrasting
confidence: 56%
“…4, an intense brown spot at R f 0·26 was detected on TLC and identified as medicoside J. This compound had also been identified by Massiot et al (19) as a bisdesmoside of medicagenic acid. Medicoside J did not appear to be present in the purified root or shoot saponin mixtures from Sdg tissues (Fig.…”
Section: Changes Of Alfalfa Saponins Extracted From Shoot Tissuesmentioning
confidence: 55%
“…Another (19) produced an olive green spot at R f 0·19. An additional, fainter, purple spot at R f 0·59 was also present in this sample, which could be identified as a 3-O-b-D-glucopyranosyl medicagenic acid in comparison with the other reference compounds.…”
Section: Identification Of Alfalfa Saponins Using Reference Compoundsmentioning
confidence: 99%
“…The shoot saponin mixture contained this compound as a major component; it was also present in a fraction eluted with 44 % iso-propanol (16) . The third bisdesmoside component, structurally identified (19) , was detected as a bluegrey spot at R f 0·26. This compound appeared to be a minor component of the root saponin mixture, present in fractions which eluted with 52 -56 % iso-propanol (16) .…”
Section: Identification Of Alfalfa Saponins Using Reference Compoundsmentioning
Biochemical components in alfalfa (Medicago sativa L.), such as saponins, can act as protecting factors against bio-stresses. Saponins are also antifeedants and show oral toxicity towards higher and lower animals. Changes in saponins, such as variation in the carbon skeleton, or hydrolysis of saponin glycosides and other conjugates, may change their biological effects. The aims of this research were to study saponin variation in different growth stages of alfalfa and to investigate the biological role of saponins in the spotted alfalfa aphid, Therioaphis maculata. Saponins from alfalfa shoots in different growth stages were extracted, chemically purified and analysed by TLC. Specific saponins such as soyasaponin1 from root and shoot and two bisdesmosides of medicagenic acid, one from shoot and another from root tissues, were identified using reference compounds allowing changes in saponin composition during plant development in different shoot tissues of alfalfa to be assessed. The response of the alfalfa aphid to feeding on alfalfa in different growth stages was studied. No significant difference in the survival of aphids, from neonate to adult, was observed, but due to the antibiotic effects of saponins, two differences were found in the onset of nymph production and cumulative nymph production. The results show that the saponin composition in alfalfa changes with plant development and this, in turn, can often negatively affect the development of specific insect pests such as the spotted alfalfa aphid, suggesting a possible biological role of alfalfa saponins.
“…A comparison between root and shoot saponin mixtures showed that this compound was purified in fractions eluted with 45-56 % iso-propanol and appears to be the major saponin present in both tissues, previously described as having R f 0·43 (16) (result not shown here). (19) gave a dark olive-coloured spot at R f 0·29, but did not appear to be present in the root or shoot saponin mixtures in the present work.…”
Section: Identification Of Alfalfa Saponins Using Reference Compoundscontrasting
confidence: 56%
“…4, an intense brown spot at R f 0·26 was detected on TLC and identified as medicoside J. This compound had also been identified by Massiot et al (19) as a bisdesmoside of medicagenic acid. Medicoside J did not appear to be present in the purified root or shoot saponin mixtures from Sdg tissues (Fig.…”
Section: Changes Of Alfalfa Saponins Extracted From Shoot Tissuesmentioning
confidence: 55%
“…Another (19) produced an olive green spot at R f 0·19. An additional, fainter, purple spot at R f 0·59 was also present in this sample, which could be identified as a 3-O-b-D-glucopyranosyl medicagenic acid in comparison with the other reference compounds.…”
Section: Identification Of Alfalfa Saponins Using Reference Compoundsmentioning
confidence: 99%
“…The shoot saponin mixture contained this compound as a major component; it was also present in a fraction eluted with 44 % iso-propanol (16) . The third bisdesmoside component, structurally identified (19) , was detected as a bluegrey spot at R f 0·26. This compound appeared to be a minor component of the root saponin mixture, present in fractions which eluted with 52 -56 % iso-propanol (16) .…”
Section: Identification Of Alfalfa Saponins Using Reference Compoundsmentioning
Biochemical components in alfalfa (Medicago sativa L.), such as saponins, can act as protecting factors against bio-stresses. Saponins are also antifeedants and show oral toxicity towards higher and lower animals. Changes in saponins, such as variation in the carbon skeleton, or hydrolysis of saponin glycosides and other conjugates, may change their biological effects. The aims of this research were to study saponin variation in different growth stages of alfalfa and to investigate the biological role of saponins in the spotted alfalfa aphid, Therioaphis maculata. Saponins from alfalfa shoots in different growth stages were extracted, chemically purified and analysed by TLC. Specific saponins such as soyasaponin1 from root and shoot and two bisdesmosides of medicagenic acid, one from shoot and another from root tissues, were identified using reference compounds allowing changes in saponin composition during plant development in different shoot tissues of alfalfa to be assessed. The response of the alfalfa aphid to feeding on alfalfa in different growth stages was studied. No significant difference in the survival of aphids, from neonate to adult, was observed, but due to the antibiotic effects of saponins, two differences were found in the onset of nymph production and cumulative nymph production. The results show that the saponin composition in alfalfa changes with plant development and this, in turn, can often negatively affect the development of specific insect pests such as the spotted alfalfa aphid, suggesting a possible biological role of alfalfa saponins.
Abstract:The diamondback moth (DBM), Plutella xylostella L. (Lepidoptera: Plutellidae) is very destructive crucifers specialized pest that has resulted in significant crop losses worldwide. The pest is well attracted to glucosinolate-containing crucifers such as; Barbarea vulgaris (Brassicaceae), and generally to other plants in the genus Barbarea. B. vulgaris on their part, build up resistance against DBM and other herbivorous insects using glucosinolates; that are plant secondary metabolites used in plant defense-contained only in plants of the order Brassicales. Aside glucosinolates, plants in this genus Barbarea (Brassicaceae) also contain saponins; which is toxic to insects and act as feeding deterrents for plant herbivores, most importantly, DBM, as it was found to prevent the survival of DBM larvae on the plant. Saponins are plant secondary metabolites have been established in higher concentrations in younger in contrast to older leaves within the same plant. Previous studies have found a relationship between ontogenetical changes in the host plant's saponin content and attraction/resistance to P. xylostella. The younger leaves recorded higher concentrations of glucosinolates and saponins, which naturally attracts the plant herbivores. DBM was reported to have evolved mechanisms to avoid the toxicity of the former. The plant-herbivore had adapted glucosinolates for host plant recognition, feeding and oviposition stimulants. Despite the adaptation for oviposition by P. xylostella adults, larvae of the insect cannot survive on the same plant. An example is in some varieties of B. vulgaris. The triterpenoid saponins which act as feeding deterrents in larvae are responsible for this direct defense mechanism against P. xylostella. In the future, trials by plant breeders could aim at transferring this insect resistance to other crops. The previous trials had limited because of lack of knowledge on the biosynthetic pathways and regulatory networks of saponins. Herein, we discussed exclusively; saponins mediated plant defense mechanisms against the DBM.
: Medicagenic acid (MA) content was studied in four Mexican, one Spanish and Ðve USA cultivars of alfalfa (Medicago sativa L) using highperformance liquid chromatography (HPLC) and two bioassays : T richoderma viride inhibition and ichthyotoxic activity. The MA contents (g kg~1), measured through HPLC, for the 10 varieties were : Valenciana \ 0É165 ; Inia 76 \ 0É115 ; Puebla 76 \ 0É097 ; Synthetic I \ 0É042 ; Pierce \ 0É031 ; Maxidor \ 0É027 ; Condor \ 0É024 ; Synthetic II \ 0É023 ; Sundor \ 0É023 ; NK-819 \ 0É013. These data conÐrm the inÑuence of the variety in MA contents. Fungitoxic and ichthyotoxic assays showed that the Spanish cultivar (Valenciana) presented the highest toxicity. The biological activity of the ten varieties was strongly correlated (r \ 0É97) to medicagenic acid content measured through HPLC.
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