Reinnervation of a denervated skeletal muscle by spinal axons regenerating through a collagen channel directly implanted into the rat spinal cord

Kassar-Duchossoy, Lina; Duchossoy, Yann; Rhrich-Haddout, Fatiha; Horvat, Jean-Claude

doi:10.1016/s0006-8993(01)02554-9

Cited by 13 publications

(11 citation statements)

References 45 publications

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“…Fulfilling these criteria were the following matrices: (a) methylcellulose (Sigma-Aldrich), a biopolymer that is a methyl ether of cellulose, which has been shown to be an effective material for neural tissue-engineering therapies, mainly in the area of drug or molecular, rather than cellular, delivery (Tate et al, 2001); (b) extracellular matrix (ECM; Sigma-Aldrich) gel composed of laminin and collagen type IV, both of which have been demonstrated to be supportive matrices for SCs (Kassar-Duchossoy et al, 2001;Pierucci et al, 2008;Stabenfeldt et al, 2006); and lastly (c) MatrigelÔ (BD), another matrix composition of laminin and collagen type IV, with molecules including heparan sulfate proteoglycans, entacin, and growth factors. BD Matrigel is derived from a gelatinous protein mixture secreted by mouse tumor cells, and has been employed conjunctively with SC grafting in models of spinal-cord hemisection (Iannotti et al, 2003) and complete transection (Fouad et al, 2005;Meijs et al, 2004;Xu et al, 1997).…”

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confidence: 99%

Suspension Matrices for Improved Schwann-Cell Survival after Implantation into the Injured Rat Spinal Cord

Patel

Joseph

Patel

et al. 2010

Journal of Neurotrauma

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Trauma to the spinal cord produces endogenously irreversible tissue and functional loss, requiring the application of therapeutic approaches to achieve meaningful restoration. Cellular strategies, in particular Schwanncell implantation, have shown promise in overcoming many of the obstacles facing successful repair of the injured spinal cord. Here, we show that the implantation of Schwann cells as cell suspensions with in-situ gelling laminin:collagen matrices after spinal-cord contusion significantly enhances long-term cell survival but not proliferation, as well as improves graft vascularization and the degree of axonal in-growth over the standard implantation vehicle, minimal media. The use of a matrix to suspend cells prior to implantation should be an important consideration for achieving improved survival and effectiveness of cellular therapies for future clinical application.

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confidence: 99%

Suspension Matrices for Improved Schwann-Cell Survival after Implantation into the Injured Rat Spinal Cord

Patel

Joseph

Patel

et al. 2010

Journal of Neurotrauma

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“…Collagen-based tubes have been also used to bridge the spinal cord to the PNS, in both rats and primates. 22,24 Collagen filaments, grafted parallel to the axis of spinal cord, have been reported to allow for some functional restoration in a rabbit SCI model. 21 The aim of this study was to validate, at a preclinical stage, the use of a novel composite device, entirely made up of type I collagen and consisting of a tubular conduit filled with two semielliptical lobes, as depicted in Figure 1, to grossly resemble the shape and structure of the spinal cord.…”

Section: Discussionmentioning

confidence: 99%

“…12 A score of 0 was given for no spontaneous hind limb movement while a score of 21 indicated normal locomotion. Locomotor activity was monitored at 1,6,11,18,24,32,39,45,50, and 55 days postinjury (dpi). Before each evaluation rats were carefully examined for infection, wounds in the hind limbs, and tail and foot autophagia.…”

Section: Behavioral Analysismentioning

confidence: 99%

A novel composite type I collagen scaffold with micropatterned porosity regulates the entrance of phagocytes in a severe model of spinal cord injury

Snider

Cavalli

Colombo

et al. 2016

J. Biomed. Mater. Res.

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Traumatic spinal cord injury (SCI) is a damage to the spinal cord that results in loss or impaired motor and/or sensory function. SCI is a sudden and unexpected event characterized by high morbidity and mortality rate during both acute and chronic stages, and it can be devastating in human, social and economical terms. Despite significant progresses in the clinical management of SCI, there remain no effective treatments to improve neurological outcomes. Among experimental strategies, bioengineered scaffolds have the potential to support and guide injured axons contributing to neural repair. The major aim of this study was to investigate a novel composite type I collagen scaffold with micropatterned porosity in a rodent model of severe spinal cord injury. After segment resection of the thoracic spinal cord we implanted the scaffold in female Sprague-Dawley rats. Controls were injured without receiving implantation. Behavioral analysis of the locomotor performance was monitored up to 55 days postinjury. Two months after injury histopathological analysis were performed to evaluate the extent of scar and demyelination, the presence of connective tissue and axonal regrowth through the scaffold and to evaluate inflammatory cell infiltration at the injured site. We provided evidence that the new collagen scaffold was well integrated with the host tissue, slightly ameliorated locomotor function, and limited the robust recruitment of the inflammatory cells at the injury site during both the acute and chronic stage in spinal cord injured rats. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1040-1053, 2017.

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“…CS has also been applied in nervous tissue engineering [69,70]. The implantation of type-I collagen/CS in the spinal cord or the peripheral nerve tissue lesions can prevent the growth of connective tissue into the lesion segment and subsequently avoid the inhibitive effect of lesions to the nerve fiber extension.…”

Section: Tissue Regeneration and Engineeringmentioning

confidence: 99%

Chondroitin Sulfate as a Bioactive Macromolecule for Advanced Biological Applications and Therapies

Volpi

2016

Biomaterials From Nature for Advanced Devices and Therapies

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Reinnervation of a denervated skeletal muscle by spinal axons regenerating through a collagen channel directly implanted into the rat spinal cord

Cited by 13 publications

References 45 publications

Suspension Matrices for Improved Schwann-Cell Survival after Implantation into the Injured Rat Spinal Cord

Suspension Matrices for Improved Schwann-Cell Survival after Implantation into the Injured Rat Spinal Cord

A novel composite type I collagen scaffold with micropatterned porosity regulates the entrance of phagocytes in a severe model of spinal cord injury

Chondroitin Sulfate as a Bioactive Macromolecule for Advanced Biological Applications and Therapies

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