Reinigung eines thermolabilen, in gramnegativen Bakterien vorkommenden Antigens (ATA) mit Hilfe der Affinitätschromatographie

Seltmann, G.; Reissbrodt, Rolf

doi:10.1002/jobm.3630140309

Z Allg Mikrobiol

1974

DOI: 10.1002/jobm.3630140309

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Reinigung eines thermolabilen, in gramnegativen Bakterien vorkommenden Antigens (ATA) mit Hilfe der Affinitätschromatographie

G. Seltmann¹,

Rolf Reissbrodt²

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1976

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Cited by 6 publications

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“…1 = 11 cm) Sepharose 4B (PEARMACIA, Uppsala, Sweden) activated with anti-ATA antibodies (SELTMANN and REISSBRODT 1974). Elution was performed as described for the purification of ATA.…”

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confidence: 99%

The anodically‐moving thermolabile antigen (ATA) of gram‐negative bacteria: Its function as the receptor for F pili

Reissbrod

Tschäpe

Seltmann

1976

J of Basic Microbiology

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“…1 = 11 cm) Sepharose 4B (PEARMACIA, Uppsala, Sweden) activated with anti-ATA antibodies (SELTMANN and REISSBRODT 1974). Elution was performed as described for the purification of ATA.…”

mentioning

confidence: 99%

The anodically‐moving thermolabile antigen (ATA) of gram‐negative bacteria: Its function as the receptor for F pili

Reissbrod

Tschäpe

Seltmann

1976

J of Basic Microbiology

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“…This solution was applied to a column ( 0 = 1.2 om. 1 = 11 cm) Sepharose 4B (PEARMACIA, Uppsala, Sweden) activated with anti-ATA antibodies (SELTMANN and REISSBRODT 1974). Elution was performed as described for the purification of ATA.…”

mentioning

confidence: 99%

The anodically-moving thermolabile antigen (ATA) of gram-negative bacteria: Its function as the receptor for F pili

1976

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The anodically-moving thermolabile antigen ATA of protein nature, present in the cell wall of all members of Enterobacteriaceae and other gram-negative bacteria tested, seems to be the F pili target on the recipient cell sufrace.The conjugal transfer of plasmids is strictly dependent on the presence of sex pili determined by the donor cell. A specific reaction between the pili and a receptor site on the cell surface is thought t o be the initial step in the conjugation process that induces all following events. The existence of such specific receptors for the sex pili function is postulated by KERN (1962)) YDRA (1962), LANCASTER et al. (1965)) IV~AUCA-CARO and COMOLLI (1965), and SCHWARTZ et al. (1965) who demonstrated the inhibition of conjugation by cell wall components. Their nature, however, has not yet been elucidated. Recently, SKURRAY et al. (1974) describsd a new class of mutants of Escherichia coli K 12 lacking a cell wall protein. Thess mutants are unable to function as recipients for several F-like plasmids. The receptor substanoe for sex pili function may be conceived as a substance common to all members of Enterobacteriaceae and other gram-negative bacteria. Such a .common antigen was described by SELTNANN (1971). He found an anodically-moving thermolabile antigen (ATA) of protein nature in Shigella sonnei and in other gram-negative bacteria. I n addition, rough mutants of E . coli and Sh. flexneri (R, -Re) have been demonstrated t o contain also ATA, though, in the case of Re, in a reduced quantity (SELTMANN and REISSBRODT 1975).This paper presents experiments suggesting that ATA may be the receptor for F pili. Material and methodsBacterial strains and plasmids: The bacterial strains and plasmids used in the experiments are listed in Tables 1 and 2. They all came from the culture collection of this institute.Bacteriophages: The bacteriophages used in these experiments are listed in Table 3. They also came from the culture collection of this institute.Nutrient media: Nahragar I and Nahrbouillon I are commercial products (SIFIN, Berlin-Weillensee, GDlt) as is Bacto nutrient broth (DIFCO Laboratories Inc., Detroit, USA). My-medium and water blue-glucose-agar used in conjugation experiments were described by CLOWES and HAYES (1968) and KIESEWALTER and SELTMANN (1968), respectively.Production of sera and y-globulin fractions: The serum containing the complete antibody spectrum ("complete serum") and the serum containing the complete spectrum, except the anti-ATA antibodies ("ATA minus serum") were obtained by immunization of rabbits with the strains Bhigella sonnei 2882167 and Proteus mirabilis 1095167, resp. (cf. KUHN et al. 1972). The serum containing antibodies only against ATA ("monospecific anti-ATA serum") was prepared by immunizing rabbits with purified ATA (REISSBRODT, unpubl.) ; the monospecificity of thi serum was tested against the complete ultrasonic extract of Proteus mirabilis 1095/67. The idens

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Das anodisch wandernde thermolabile Antigen (ATA) von gramnegativen Bakterien: Seine molekulare Heterogenität und seine chemische Zusammensetzung

Reissbrodt

Kemmer

Seltmann

1976

J of Basic Microbiology

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The anodically‐moving thermolabile antigen (ATA) of gram‐negative bacteria represents a glycolipoprotein. In aqueous solution ATA exhibits heterogeneity of the molecular size, the range varying between 2 · 104 and 8 · 105 Daltons. In the molecular range between 5 · 104 and 8 · 105 all the molecular states give precipitation lines with the corresponding antisera. It could be shown that all the molecular states of ATA are formed by different aggregation of the same subunit with a molecular weight of approximativly 22 000.

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Reinigung eines thermolabilen, in gramnegativen Bakterien vorkommenden Antigens (ATA) mit Hilfe der Affinitätschromatographie

Cited by 6 publications

References 4 publications

The anodically‐moving thermolabile antigen (ATA) of gram‐negative bacteria: Its function as the receptor for F pili

The anodically‐moving thermolabile antigen (ATA) of gram‐negative bacteria: Its function as the receptor for F pili

The anodically-moving thermolabile antigen (ATA) of gram-negative bacteria: Its function as the receptor for F pili

Das anodisch wandernde thermolabile Antigen (ATA) von gramnegativen Bakterien: Seine molekulare Heterogenität und seine chemische Zusammensetzung

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