Rehydration of active dried yeast: impact on strength and stiffness of yeast cells measured using microelectromechanical systems

Barazani, Bruno; Piercey, Marta; Paulson, Allan T.; Warnat, Stephan; Hubbard, Ted; MacIntosh, Andrew J.

doi:10.1002/jib.548

Cited by 2 publications

(2 citation statements)

References 27 publications

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“…Some devices need silicon-glass bonding [34], while some others use PDMS to form a channel [97]. In addition, several devices were fabricated with the PolyMUMPs TM process [95,98,99]. PolyMUMPs TM , the acronym for the polysilicon multiuser micromachining process, is a commercially available micromachining process.…”

Section: Mems Squeezersmentioning

confidence: 99%

Fabricating Silicon Resonators for Analysing Biological Samples

et al. 2021

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The adaptability of microscale devices allows microtechnologies to be used for a wide range of applications. Biology and medicine are among those fields that, in recent decades, have applied microtechnologies to achieve new and improved functionality. However, despite their ability to achieve assay sensitivities that rival or exceed conventional standards, silicon-based microelectromechanical systems remain underutilised for biological and biomedical applications. Although microelectromechanical resonators and actuators do not always exhibit optimal performance in liquid due to electrical double layer formation and high damping, these issues have been solved with some innovative fabrication processes or alternative experimental approaches. This paper focuses on several examples of silicon-based resonating devices with a brief look at their fundamental sensing elements and key fabrication steps, as well as current and potential biological/biomedical applications.

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Section: Mems Squeezersmentioning

confidence: 99%

Fabricating Silicon Resonators for Analysing Biological Samples

et al. 2021

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“…Robot-integrated microfluidic chips have immense potential for exploring the biophysical properties of cells. These chips integrate a series of functional components that allow for cell separation, capture, detection, and several other tasks for single cells [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 ]. For instance, an optical tweezers system was used to trap or stretch a single cell [ 2 , 6 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Integration of Microfluidic Chip and Probe with a Dual Pump System for Measurement of Single Cells Transient Response

Kaneko

Maruyama

et al. 2023

Micromachines

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The integration of liquid exchange and microfluidic chips plays a critical role in the biomedical and biophysical fields as it enables the control of the extracellular environment and allows for the simultaneous stimulation and detection of single cells. In this study, we present a novel approach for measuring the transient response of single cells using a system integrated with a microfluidic chip and a probe with a dual pump. The system was composed of a probe with a dual pump system, a microfluidic chip, optical tweezers, an external manipulator, an external piezo actuator, etc. Particularly, we incorporated the probe with the dual pump to allow for high-speed liquid change, and the localized flow control enabled a low disturbance contact force detection of single cells on the chip. Using this system, we measured the transient response of the cell swelling against the osmotic shock with a very fine time resolution. To demonstrate the concept, we first designed the double-barreled pipette, which was assembled with two piezo pumps to achieve a probe with the dual pump system, allowing for simultaneous liquid injection and suction. The microfluidic chip with on-chip probes was fabricated, and the integrated force sensor was calibrated. Second, we characterized the performance of the probe with the dual pump system, and the effect of the analysis position and area of the liquid exchange time was investigated. In addition, we optimized the applied injection voltage to achieve a complete concentration change, and the average liquid exchange time was achieved at approximately 3.33 ms. Finally, we demonstrated that the force sensor was only subjected to minor disturbances during the liquid exchange. This system was utilized to measure the deformation and the reactive force of Synechocystis sp. strain PCC 6803 in osmotic shock, with an average response time of approximately 16.33 ms. This system reveals the transient response of compressed single cells under millisecond osmotic shock which has the potential to characterize the accurate physiological function of ion channels.

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Rehydration of active dried yeast: impact on strength and stiffness of yeast cells measured using microelectromechanical systems

Cited by 2 publications

References 27 publications

Fabricating Silicon Resonators for Analysing Biological Samples

Fabricating Silicon Resonators for Analysing Biological Samples

Integration of Microfluidic Chip and Probe with a Dual Pump System for Measurement of Single Cells Transient Response

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