2013
DOI: 10.1590/s0074-02762013000100014
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Regulatory volume decrease in Leishmania mexicana: effect of anti-microtubule drugs

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Cited by 3 publications
(3 citation statements)
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References 37 publications
(42 reference statements)
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“…Quantification demonstrated a ∼1.5-fold increase in flagella length (Figure S1B, p < 0.05 by ANOVA compared to DMSO control) when promastigotes were treated with MMV676477, paclitaxel, or active analogs for 24 h at their respective EC 50 72h . We also found that promastigotes treated with paclitaxel, MMV676477, or active analogs at their respective EC 50 72h for 48 h were more rounded or pear-shaped (defined as parasites with a cell body width that was ≥70% of the cell body length 29 ) than DMSO controls or promastigotes treated with the unrelated drug, miltefosine (Figure S1C). Quantification demonstrated a 2-fold increase in pear-shaped promastigotes in paclitaxel, MMV676477, and active analog-treated groups (Figure S1D, p < 0.05 by ANOVA compared to DMSO control).…”
Section: Screening the Pathogen Box Identifies Multiple Hitmentioning
confidence: 79%
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“…Quantification demonstrated a ∼1.5-fold increase in flagella length (Figure S1B, p < 0.05 by ANOVA compared to DMSO control) when promastigotes were treated with MMV676477, paclitaxel, or active analogs for 24 h at their respective EC 50 72h . We also found that promastigotes treated with paclitaxel, MMV676477, or active analogs at their respective EC 50 72h for 48 h were more rounded or pear-shaped (defined as parasites with a cell body width that was ≥70% of the cell body length 29 ) than DMSO controls or promastigotes treated with the unrelated drug, miltefosine (Figure S1C). Quantification demonstrated a 2-fold increase in pear-shaped promastigotes in paclitaxel, MMV676477, and active analog-treated groups (Figure S1D, p < 0.05 by ANOVA compared to DMSO control).…”
Section: Screening the Pathogen Box Identifies Multiple Hitmentioning
confidence: 79%
“…Since we obtained MMV676477 from a phenotypic screen, our next goal was to identify its likely target. Upon review of the literature, we noted that MMV676477-treated L. amazonensis microscopically resembled Leishmania treated with the anticancer drug paclitaxel (“Taxol”, EC 50 72h for La = 760 ± 8.3 nM) . Paclitaxel stabilizes microtubules and prevents mitosis in cancer cells .…”
Section: Resultsmentioning
confidence: 99%
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