Regulatory role of microRNA in mesenteric lymph nodes after Salmonella Typhimurium infection

Abstract

Salmonellosis is a gastrointestinal disease caused by non-typhoidal Salmonella serovars such as Salmonella Typhimurium. This pathology is a zoonosis, and food animals with subclinical infection constitute a vast reservoir for disease. After intestinal colonization, Salmonella Typhimurium reaches mesenteric lymph nodes (MLN), where infection is controlled avoiding systemic spread. Although the molecular basis of this infection has been extensively studied, little is known about how microRNA (miRNA) regulate the expression of proteins involved in the Salmonella-host interaction. Using small RNA-seq, we examined expression profiles of MLN 2 days after infection with Salmonella Typhimurium, and we found 110 dysregulated miRNA. Among them, we found upregulated miR-21, miR-155, miR-150, and miR-221, as well as downregulated miR-143 and miR-125, all of them previously linked to other bacterial infections. Integration with proteomic data revealed 30 miRNA potentially regulating the expression of 15 proteins involved in biological functions such as cell death and survival, inflammatory response and antigenic presentation. The inflammatory response was found increased via upregulation of miRNA such as miR-21 and miR-155. Downregulation of miR-125a/b, miR-148 and miR-1 were identified as potential regulators of MHC-class I components PSMB8, HSP90B1 and PDIA3, respectively. Furthermore, we confirmed that miR-125a is a direct target of immunoproteasome component PSMB8. Since we also found miR-130 downregulation, which is associated with upregulation of HSPA8, we suggest induction of both MHC-I and MHC-II antigen presentation pathways. In conclusion, our study identifies miRNA that could regulate critical networks for antigenic presentation, inflammatory response and cytoskeletal rearrangements.Electronic supplementary materialThe online version of this article (10.1186/s13567-018-0506-1) contains supplementary material, which is available to authorized users.

Chlorogenic Acid Promotes Autophagy and Alleviates Salmonella Typhimurium Infection Through the lncRNAGAS5/miR-23a/PTEN Axis and the p38 MAPK Pathway

“…The ceRNA network of GAS5 has been reported (Liu et al, 2019;Fang et al, 2020). After literature search, we found that there were many abnormal expression of miR in mesenteric lymph nodes after ST infection (Herrera-Uribe et al, 2018). Additionally, biological website prediction, RNA pull-down and dual luciferase reporter gene assays confirmed that miR-23a has binding relation to GAS5.…”

“…GAS5 can play a role in regulating diseases through the ceRNA mechanism. Through literature search, we found that there were many abnormal expression of miR in mesenteric lymph nodes after ST infection ( Herrera-Uribe et al, 2018 ). Therefore, we screened these miRs in the biological websites, and found miR-23a has binding relation to GAS5 ( Figure 6E ).…”

Functional Screening Identifies MicroRNA Regulators of Corin Activity and Atrial Natriuretic Peptide Biogenesis

“…3b, Table S2). These mRNAs included 5 validated miR-1-3p targets ( CDC42 [30], FN1 [31], PDIA3 [32], SARS [33], and SOD1 [34]) and 27 predicted targets (based on results from the miRNA target prediction tool Targetscan 7.2) (Table S2) (35). Interestingly, we also identified CORIN itself as one of the enriched miR-1/AGO2-associated mRNAs.…”

MicroRNAs as new immunity regulators in viral and bacterial infections

“…This molecule is referred to as the master regulator of inflammation (Mahesh, Biswas, 2019). The high expression of miRNA-155 was reported in monocytes, macrophages, T and B lymphocytes, NK cells and DC (Dickey et al, 2017;Herrera-Uribe et al, 2018;King et al, 2016). In the above-mentioned cells, miRNA-155 is responsible for regulating the secretion of chemokines, cytokines and transcription factors in such a way that the immune response is optimal (Dickey et al, 2017).…”

“…Its presence in the body may lead to the development of typhoid fever or gastroenteritis (Naveed et al, 2017;Zhou et al, 2018). It is usually transmitted through food (mainly on products of animal origin contaminated with faeces) (Herrera-Uribe et al, 2018;Szewczyk et al, 2013). There are three main serotypes of Salmonella: Typhi, Typhimurium and Enteritidis (Zhou et al, 2018).…”