Regulatory properties and cellular redistribution of zinc during macrophage differentiation of human leukemia cells

Glesne, David; Vogt, Stefan; Mäser, J.; Legnini, D.; Huberman, Eliezer

doi:10.1016/j.jsb.2005.09.012

Cited by 33 publications

(31 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of the 36 scans of vascularized regions, 10 appeared representative of lymphatic space invasion, 15 were axial cuts of mature vessels, and 10 were parallel cuts of mature vessels. Nonvascularized breast tissue displayed elemental content roughly uniformly distributed according to cell thickness, resulting in images that appear similar to those obtained for other eukaryotic cells, with individual cells clearly visible in P, S, Ca, Fe, Cu, and Zn maps (22) (Fig. 4, breast tissue).…”

Section: Resultssupporting

confidence: 66%

“…XFM analysis of these control cells demonstrated elemental distributions for P, S, and Zn typical of eukaryotic cells, with S correlating to overall cell thickness and Zn most prevalent in the nucleus (Fig. 1, Flat) (22). Because of the high background content of Fe in the surrounding matrix, cellular distribution maps for this metal were uninformative for the in vitro samples.…”

Section: Resultsmentioning

confidence: 99%

“…Residual PBS was removed by several washes in 20 mM Pipes, pH 7.2/200 mM sucrose followed by air drying. This method of cell fixation and preparation for x-ray fluorescence to minimize disruption of metal ion topology has been previously described (22) and does not significantly alter typical cellular copper content relative to fixation by plunge …”

Section: Methodsmentioning

confidence: 99%

“…Most of the initial biological applications of XFM analysis to date have focused on the distribution of nonendogenous metals within the cellular space, such as the localization of TiO 2 -labeled oligonucleotides (17), the examination of platinum oxidation states (18), and bacterial uptake of chromium (19). However, more recent work has begun to demonstrate the utility of this technology for the quantitation and localization of endogenous metals, such as in studies on the phagosome environment in pathogen-infected macrophages (20), on the copper content and topography of fibroblasts (21), and on the relocalization of zinc during early stages of macrophage differentiation (22). We have used XFM to explore the relationship between copper and angiogenesis both in vitro and in vivo and have discovered that massive relocalization of cellular copper stores appears to be a requirement for proper angiogenic network formation.…”

mentioning

confidence: 99%

See 3 more Smart Citations

X-ray fluorescence microscopy reveals large-scale relocalization and extracellular translocation of cellular copper during angiogenesis

Finney

Mandava

Ursos

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

176

142

View full text Add to dashboard Cite

Although copper has been reported to influence numerous proteins known to be important for angiogenesis, the enhanced sensitivity of this developmental process to copper bioavailability has remained an enigma, because copper metalloproteins are prevalent and essential throughout all cells. Recent developments in x-ray optics at third-generation synchrotron sources have provided a resource for highly sensitive visualization and quantitation of metalloproteins in biological samples. Here, we report the application of x-ray fluorescence microscopy (XFM) to in vitro models of angiogenesis and neurogenesis, revealing a surprisingly dramatic spatial relocalization specific to capillary formation of 80 -90% of endogenous cellular copper stores from intracellular compartments to the tips of nascent endothelial cell filopodia and across the cell membrane. Although copper chelation had no effect on process formation, an almost complete ablation of network formation was observed. XFM of highly vascularized ductal carcinomas showed copper clustering in putative neoangiogenic areas. This use of XFM for the study of a dynamic developmental process not only sheds light on the copper requirement for endothelial tube formation but highlights the value of synchrotron-based facilities in biological research.copper chelation ͉ human microvascular endothelial cells ͉ infiltrating ductal breast carcinoma E ndogenous metals, such as Cu, Fe, and Zn, are subject to complex regulation in cellular systems. They are required as cofactors or regulators of numerous proteins (1) and yet, if present in overabundance, are toxic and expose the cellular environment to adventitious redox activity (2). This delicate balance is thought to be achieved by sequestration of these metals within their target proteins, metallochaperone systems, or distinct subcellular compartments (3). Although many proteins that handle transition metals within cells have been identified (4), our knowledge as to how metal content is dynamically regulated in eukaryotic cells is still limited. To what extent does regulation of the metal ion content of individual metalloproteins, mediated by protein-protein interactions, serve as an additional level of regulation of cellular metalloprotein activity? Could such regulation result in polarization of transition metal distribution throughout a cell during a biological process? To begin to explore such questions, we examined a cellular system whose biology is acutely sensitive to modulation by metals.

show abstract

Section: Resultssupporting

confidence: 66%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

X-ray fluorescence microscopy reveals large-scale relocalization and extracellular translocation of cellular copper during angiogenesis

Finney

Mandava

Ursos

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

176

142

View full text Add to dashboard Cite

show abstract

“…One of the first findings regarding zinc (utilizing sub-micron X-ray fluorescence imaging) was that it may be involved in cell differentiation, particularly looking at HL-60 cells (Glesne, Vogt et al 2006). In examining the growth of human embryonic stem cells, taking a systems biology approach to examining entire colonies of cells and all the first row transition metals, we also found that the amount of zinc present in cells directly correlated with their differentiation (Wolford, Chishti et al 2010).…”

Section: A New View Of Zinc From Xfmmentioning

confidence: 71%

Inorganic Signatures of Physiology: The X-Ray Fluorescence Microscopy Revolution

Finney¹

2012

Biomarker

View full text Add to dashboard Cite

Metal Homeostasis

Outten¹,

Twining²

2008

Wiley Encyclopedia of Chemical Biology

View full text Add to dashboard Cite

Transition metals are a key component of biological systems. Because of their special properties, they are incorporated into proteins functioning in dioxygen transport, electron transfer, redox transformations, and regulatory control. The metals used in biological systems have been selected throughout evolution based on their availability in the environment and their kinetic lability, resulting in preferential use of first‐row transition metals in biology. These essential metals must be obtained from the environment and concentrated within the cell for use in biochemical pathways. Once appropriated, metals must be directed to metalloenzymes or metal storage proteins within the cell. In addition, organisms must be able to distinguish between essential and toxic metals and must have mechanisms for minimizing the toxicity of both essential and toxic metals that are present in excess. Metal homeostasis is broadly defined as the metal uptake, trafficking, efflux, and sensing pathways that allow organisms to maintain an appropriate (often narrow) intracellular concentration range of essential transition metals. This review will introduce several unifying concepts of metal homeostasis with brief illustrative examples for each concept.

show abstract

Regulatory properties and cellular redistribution of zinc during macrophage differentiation of human leukemia cells

Cited by 33 publications

References 36 publications

X-ray fluorescence microscopy reveals large-scale relocalization and extracellular translocation of cellular copper during angiogenesis

X-ray fluorescence microscopy reveals large-scale relocalization and extracellular translocation of cellular copper during angiogenesis

Inorganic Signatures of Physiology: The X-Ray Fluorescence Microscopy Revolution

Metal Homeostasis

Contact Info

Product

Resources

About