Regulatory non-coding RNAs: Emerging roles during plant cell reprogramming and in vitro regeneration

Cordeiro, Daniela; Canhoto, Jorge; Correia, Sandra

doi:10.3389/fpls.2022.1049631

Cited by 5 publications

(2 citation statements)

References 86 publications

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“…The current understanding of the roles that several RNAs play in plant regeneration processes holds promise for reprogramming cell fate. Furthermore, the involvement and effects of small interfering RNAs (siRNAs), long non-coding RNAs (lncRNAs), and microR-NAs (miRNAs) showed a significant impact on the cell's differentiation, proliferation, and de-differentiation regulatory networks [205][206][207]. Plant tissues exhibit a general increase in miRNA levels during the de-differentiation phase [208].…”

Section: Insights and New Technologies For In Vitro Culture Of Gladiolusmentioning

confidence: 99%

“…Therefore, these points should be considered prior to initiating in vitro work on the gladiolus. Since no research of this kind has been done on gladiolus, modern molecular tools such as CRISPR Cas [202] and RNAi [207] are also used in in vitro culture for genetic improvement in plants. As a result, these methods using in vitro culture are more successful for genetic improvement in gladiolus.…”

Section: Conclusion and Future Prospectsmentioning

confidence: 99%

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In Vitro Propagation Journey of Ornamental Gladiolus (Gladiolus Species): A Systematic Review Analysis Based on More Than 50 Years Research

Kumar,

Chaudhary,

Sirohi

et al. 2024

Horticulturae

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Traditional gladiolus propagation methods are now supplemented with in vitro propagation to meet the demands of modern floriculture in terms of quick production of disease-free, quality planting material. Due to virus infections, vegetative propagation in gladiolus in the field is slow, and is a serious concern in the propagation of gladiolus. In vitro propagation provides an enormous increase in propagation rate and the ability to produce disease-free plant material. Numerous elements, including cultivars, explant type, size of explants, position of explants on medium, plant growth regulators and certain additives, incubation conditions, and sub-culturing time, all have a significant impact on in vitro clonal propagation of gladiolus plants as well as the development of in vitro cormel efficiency. There are certain obstacles and challenges that arise in the in vitro development of plants and the cormels of gladiolus. However, numerous studies and review reports on gladiolus for in vitro propagation have been reported, but very little is known about the factors influencing gladiolus’ in vitro effectiveness. In the present review, we focused on and analyzed research data accumulated over 50 years on diverse strategies for in vitro propagation such as direct, indirect organogenesis, and somatic embryogenesis, as well as various factors such as physical, nutritional, and hormonal influences on in vitro propagation, in vitro cormel formation efficiency, difficulties that arise, and new insights into in vitro development in gladiolus from the available literature worldwide. Future possibilities for further improvement in the in vitro propagation of ornamental gladiolus are also discussed. The current review provides insight into a comprehensive protocol for gladiolus in vitro propagation and emphasizes the importance of continuously advancing tissue culture techniques and factors influencing the in vitro efficiency towards improving in vitro plantlets and cormels in gladiolus (Gladiolus spp.).

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Section: Insights and New Technologies For In Vitro Culture Of Gladiolusmentioning

confidence: 99%

Section: Conclusion and Future Prospectsmentioning

confidence: 99%

In Vitro Propagation Journey of Ornamental Gladiolus (Gladiolus Species): A Systematic Review Analysis Based on More Than 50 Years Research

Kumar,

Chaudhary,

Sirohi

et al. 2024

Horticulturae

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Analysis of the potential involvement of lncRNAs in embryogenic competence of Solanum betaceum Cav. (tamarillo) calli

Cordeiro,

Camelo,

Pedrosa

et al. 2024

Plant Cell Tiss Organ Cult

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Somatic embryogenesis (SE) is a process by which somatic cells reprogram, acquire totipotency and embark on embryo formation. Although SE is a valuable tool for micropropagation in many crops, it presents specific challenges in woody species due to some bottlenecks, such as loss of embryogenic competence during subcultures and the often-low somatic embryo conversion into plantlets. Hence, great interest exists in exploring the regulatory networks involved on SE. As transcription modulators, long non-coding RNAs (lncRNAs) have been found essential in a wide range of biological processes. This work aimed to identify lncRNAs related to the embryogenic competence in Solanum betaceum Cav. (tamarillo). Nanopore® long-read sequencing was conducted in cell lines with distinct cell fates and, based on their coding potential, 60 transcripts were selected as lncRNA candidates. Similar expression patterns were found among embryogenic cell lines (EC) and cells that lost their embryogenic potential (herein long-term callus, LTC), whereas non-EC (NEC) showed differentially expressed lncRNAs. Whereas lncRNAs upregulated in EC and LTC were predicted to target embryogenesis-related genes, such as AGAMOUS-like 15 and WUSCHEL-related HOMEOBOX 2, lncRNAs upregulated in NEC were predicted to target mainly genes involved in carbohydrate metabolism, cell wall formation, auxin and ethylene signalling pathways. Altogether, these results show the involvement of lncRNA in the process of somatic embryogenesis in S. betaceum, indicating that EC present a pattern of lncRNA expression that suggests its action on genes that directly modulate the morphogenic response in vitro, while in NEC the modulation through this type of RNAs seems to be predominantly reflected in genes more related to cellular physiology. Graphical Abstract

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ncRNAs in plant development and stress responses

Gill,

Khan,

Agarwala

et al. 2024

Plant Physiology and Biochemistry

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Regulatory non-coding RNAs: Emerging roles during plant cell reprogramming and in vitro regeneration

Cited by 5 publications

References 86 publications

In Vitro Propagation Journey of Ornamental Gladiolus (Gladiolus Species): A Systematic Review Analysis Based on More Than 50 Years Research

In Vitro Propagation Journey of Ornamental Gladiolus (Gladiolus Species): A Systematic Review Analysis Based on More Than 50 Years Research

Analysis of the potential involvement of lncRNAs in embryogenic competence of Solanum betaceum Cav. (tamarillo) calli

ncRNAs in plant development and stress responses

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