Regulatory mechanisms of phytotoxicity and corona formation on sprouts by differently charged and sized polystyrene micro/nano-plastics

Kang, Mengen; Gong, Dongqing; Weng, Yuzhu; Ying, Huang; Chen, Yanling; Li, Fengjie; Bai, Xue

doi:10.1039/d2en00915c

Cited by 5 publications

(3 citation statements)

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“…Exudates and NP Surfaces. After 10 days of NP exposure, the plants in square seedling trays were shaken and removed to ensure maximum shedding of NPs from the root surface into the suspension, and the suspension was homogenized by treating with ultrasound (40 kHz) for 10 min 36 The obtained suspension of ZnO NP− root exudate complexes was stored at −80 °C for subsequent utilization. A 100 mL aliquot of complex suspension was added to a centrifuge tube and centrifuged at 14,000 rpm for 30 min at 4 °C, with samples prepared for each treatment group in triplicate.…”

Section: Interactions Between Rootmentioning

confidence: 99%

“…Physiological inhibition of roots reportedly impedes the transport of nutrient components to shoots, 36 which potentially affects photosynthesis and chlorophyll fluorescence. Specifically, PCA divided the photosynthetic system indicators into three components, accounting for 53.07, 20.01, and 10.96%, respectively (Figure S3a).…”

Section: Apoptosis and Photosynthetic Alterationsmentioning

confidence: 99%

“…In natural ecosystems, plants continuously release complex root exudates, i.e., active metabolites containing organic acids, amino acids, proteins, sugars, and nucleic acids, during growth. 36 The toxicological effects induced by different NPs can not only modify biological processes but also stimulate the release of root exudates, 22 which inevitably interact with exposed NPs in the environment, possibly contributing to the generation of NP−corona complexes. Nevertheless, there is an enormous lack of research on the metabolism and corona evolution of root−nano systems under the modulation of NPs with different surface properties.…”

Section: Introductionmentioning

confidence: 99%

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Driving Role of Zinc Oxide Nanoparticles with Different Sizes and Hydrophobicity in Metabolic Response and Eco-Corona Formation in Sprouts (Vigna radiata)

Kang,

Bai,

Liu

et al. 2024

Environ. Sci. Technol.

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Zinc oxide nanoparticles (ZnO NPs) cause biotoxicity and pose a potential ecological threat; however, their effects on plant metabolism and eco-corona evolution between NPs and organisms remain unclear. This study clarified the molecular mechanisms underlying physiological and metabolic responses induced by three different ZnO NPs with different sizes and hydrophobicity in sprouts (Vigna radiata) and explored the critical regulation of eco-corona formation in root–nano systems. Results indicated that smaller-sized ZnO inhibited root elongation by up to 37.14% and triggered oxidative burst and apoptosis. Metabolomics confirmed that physiological maintenance after n-ZnO exposure was mainly attributed to the effective stabilization of nitrogen fixation and defense systems by biotransformation of the flavonoid pathway. Larger-sized or hydrophobic group-modified ZnO exhibited low toxicity in sprouts, with 0.89-fold upregulation of citrate in central carbon metabolism. This contributed to providing energy for resistance to NP stress through amino acid and carbon/nitrogen metabolism, accompanied by changes in membrane properties. Notably, smaller-sized and hydrophobic NPs intensely stimulated the release of root metabolites, forming corona complexes with exudates. The hydrogen-bonded wrapping mechanism in protein secondary structure and hydrophobic interactions of heterogeneous functional groups drove eco-corona formation, along with the corona evolution intensity of n-ZnO > s-ZnO > b-ZnO based on higher (α-helix + 3-turn helix)/β-sheet ratios. This study provides crucial insight into metabolic and eco-corona evolution in bionano fates.

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Section: Interactions Between Rootmentioning

confidence: 99%

Section: Apoptosis and Photosynthetic Alterationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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