Regulatory mechanism of miR-722 on C5aR1 and its functions against bacterial inflammation in half-smooth tongue sole (Cynoglossus semilaevis)

Liu, Hongning; Tan, Suxu; Chen, Yadong; Chen, Xuejie; Liu, Xinbao; Li, Zhujun; Wang, Ningning; Han, Sen; Wu, Zhendong; Ma, Jie; Shi, Kunpeng; Wang, Wenwen; Sha, Zhenxia

doi:10.1016/j.ijbiomac.2023.126445

Cited by 8 publications

(1 citation statement)

References 85 publications

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“…The reaction system was configured on ice according to the instructions, and the reaction procedure was as follows: 95°C for 30 s; 95°C for 10 s, 60°C for 30 s, 40 cycles; 95°C for 15 s; 60°C for 1 min and 95°C for 15 s. Relative gene expression was analyzed by 2 -ΔΔCt . The 18S rRNA gene was used as the internal reference gene, as previously described (Liu et al, 2023;Wang, W. et al, 2023).…”

Section: Qrt-pcr Validationmentioning

confidence: 99%

m6A methylation dynamically participates in the immune response againstVibrio anguillarumin half-smooth tongue sole (Cynoglossus semilaevis)

Tan,

Wang,

Han

et al. 2024

Preprint

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N6-methyladenosine (m6A) is the most prevalent RNA modification and a multifaceted regulator capable of affecting various aspects of mRNA metabolism, thereby playing important roles in numerous physiological processes. However, it is still unknown whether, when, and to what extent m6A modulation are implicated in the immune response of an economically important aquaculture fish, half-smooth tongue sole (Cynoglossus semilaevis). Herein, we systematically profiled and characterized the m6A epitranscriptome and transcriptome inC. semilaevisafter the infection ofVibrio anguillarum, a serious threat to the aquaculture industry. We demonstrated that m6A could be modulated as early as 4-hour post infection (hpi), and the overall intensity of m6A methylation was enhanced following infection. Both conservative and novel motifs were uncovered from the m6A modification sites. Furthermore, differentially m6A methylated genes (DMGs) and differentially expressed genes (DEGs) were identified, and functional enrichment revealed multiple immune-related pathways, especially the FoxO signaling pathway which showed significance in every comparison. Joint analysis highlighted the remarkedly dynamic role of m6A on gene expression, i.e. early on, m6A mainly prioritized the down-regulation of specific genes, and later, it switched gears to promote expression of another set of genes. Moreover, key immune-related genes, includingpdp1,rgs5l, andplk2b, were identified. To our limited knowledge, this is the first study comprehensively characterizing the global m6A atlas in aquaculture fish species. The presented results provide new insights into the dynamics of m6A modifications in the transcriptome of the half-smooth tongue sole following bacterial infection. Further studies are warranted to elucidate the functional significance of these changes and to understand how they affect specific biological processes.

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Section: Qrt-pcr Validationmentioning

confidence: 99%