Regulatory interplay of RpoS and RssB controls motility and colonization in Vibrio cholerae

Wölflingseder, Martina; Tutz, Sarah; Fengler, Vera H.; Schild, Stefan; Reidl, Joachim

doi:10.1016/j.ijmm.2022.151555

Cited by 7 publications

(13 citation statements)

References 80 publications

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“…We established that only simultaneous overexpression of cyaA and crp could restore the levels of rpoS transcription in the degS knockout strain ( Figure 3A ), which accordingly leads us to speculate that DegS may regulate the expression of RpoS by regulating the cAMP–CRP complex. RpoS has been identified as an important regulator of flagellum biosynthesis in Yersinia ( Guan et al, 2015 ), and in V. cholerae , the interaction between RpoS and its antiregulator RssB has been shown to influence motility and colonization ability ( Wurm et al, 2017 ; Wölflingseder et al, 2022 ). We also established that the motility and colonization of V. cholerae are suppressed by the deletion of rpoS ( Figures 3B , C , 5B ).…”

Section: Discussionmentioning

confidence: 99%

DegS protease regulates the motility, chemotaxis, and colonization of Vibrio cholerae

et al. 2023

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In bacteria, DegS protease functions as an activating factor of the σE envelope stress response system, which ultimately activates the transcription of stress response genes in the cytoplasm. On the basis of high-throughput RNA sequencing, we have previously found that degS knockout inhibits the expression of flagellum synthesis- and chemotaxis-related genes, thereby indicating that DegS may be involved in the regulation of V. cholerae motility. In this study, we examined the relationships between DegS and motility in V. cholerae. Swimming motility and chemotaxis assays revealed that degS or rpoE deletion promotes a substantial reduction in the motility and chemotaxis of V. cholerae, whereas these activities were restored in ΔdegS::degS and ΔdegSΔrseA strains, indicating that DegS is partially dependent on σE to positively regulate V. cholerae activity. Gene-act network analysis revealed that the cAMP–CRP–RpoS signaling pathway, which plays an important role in flagellar synthesis, is significantly inhibited in ΔdegS mutants, whereas in response to the overexpression of cyaA/crp and rpoS in the ΔdegS strain, the motility and chemotaxis of the ΔdegS + cyaA/crp and ΔdegS + rpoS strains were partially restored compared with the ΔdegS strain. We further demonstrated that transcription levels of the flagellar regulatory gene flhF are regulated by DegS via the cAMP–CRP–RpoS signaling pathway. Overexpression of the flhF gene in the ΔdegS strain partially restored motility and chemotaxis. In addition, suckling mouse intestinal colonization experiments indicated that the ΔdegS and ΔrpoE strains were characterized by the poor colonization of mouse intestines, whereas colonization efficacy was restored in the ΔdegSΔrseA, ΔdegS + cyaA/crp, ΔdegS + rpoS, and ΔdegS + flhF strains. Collectively, our findings indicate that DegS regulates the motility and chemotaxis of V. cholerae via the cAMP–CRP–RpoS–FlhF pathway, thereby influencing the colonization of suckling mouse intestines.

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Section: Discussionmentioning

confidence: 99%

DegS protease regulates the motility, chemotaxis, and colonization of Vibrio cholerae

et al. 2023

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“…RpoS is an alternative sigma factor related to stress response and entry in stationary phase. It is a major regulator of cell physiology and influences many relevant phenotypes, such as oxidative stress, natural competence, motility, or colonization 39–41 . V. cholerae ΔSI harbored a single-base insertion in rpoS giving rise to a frameshift.…”

Section: Resultsmentioning

confidence: 99%

Chromosomal Integrons are Genetically and Functionally Isolated Units of Genomes

Blanco,

da Roza,

Toribio-Celestino

et al. 2023

Preprint

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Integrons are genetic elements that increase the evolvability of bacteria by capturing new genes and stockpiling them in arrays. Sedentary chromosomal integrons (SCIs), can be massive and highly stabilized structures encoding hundreds of genes, whose function remains generally unknown. SCIs have co-evolved with the host for aeons and are highly intertwined with their physiology from a mechanistic point of view. But, paradoxically, other aspects, like their variable content and location within the genome, suggest a high genetic and functional independence. In this work, we have explored the connection of SCIs to their host genome using as a model the Superintegron (SI), a 179-cassette long SCI in the genome ofVibrio choleraeN16961. We have relocated and deleted the SI using SeqDelTA, a novel method that allows to counteract the strong stabilization conferred by toxin-antitoxin systems within the array. We have characterized in depth the impact inV. cholerae’sphysiology, measuring fitness, chromosome replication dynamics, persistence, transcriptomics, phenomics and virulence. The deletion of the SI did not produce detectable effects in any condition, proving that -despite millions of years of co-evolution-, SCIs are genetically and functionally isolated units of genomes.

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“…Many of these genes encode global regulators, including LysR-type transcriptional regulators, LuxR family transcriptional regulators, AraC-type regulatory proteins, TetR family transcriptional regulators, the PadR-family of regulators, and two-component response regulators, as well as other genes encoding regulators with specific functions, such as the cold shock transcriptional regulator CspA. Of these, the genes encoding the two-component system response regulator RssB (VP2183), which represses motility and colonization in V. cholerae ( 63 ); LysR family transcriptional regulators (VP2427 and VPA1687); a LuxR family transcriptional regulator (VPA0358); an AraC transcriptional regulator QsvR (VPA0606), which activates the T3SS1, T6SS2, and Vp-PAI genes but represses biofilm formation by V. parahaemolyticus ( 15 , 64 , 65 ); and a response regulator (VPA1130) showed expression levels in wrinkly colonies more than 20 times higher than those in smooth colonies. In contrast, genes encoding a TetR family transcriptional regulator (VP0040), which might be involved in regulating numerous aspects of bacterial pathways such as metabolism, antibiotic resistance, and quorum sensing ( 66 ), and a putative transcriptional regulator (VP0399) showed expression levels in wrinkly colonies more than three times lower than those in smooth colonies.…”

Section: Resultsmentioning

confidence: 99%

Transcriptomic Analysis of Vibrio parahaemolyticus Underlying the Wrinkly and Smooth Phenotypes

Zhang

et al. 2022

Microbiol Spectr

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We showed that Vibrio parahaemolyticus was able to naturally and reversibly switch between wrinkly and smooth phenotypes and disclosed the gene expression profiles related to wrinkly-smooth switching, showing that the significantly differentially expressed genes between the two colony morphology phenotypes were involved in various biological behaviors, including virulence factor production, biofilm formation, metabolism, adaptation, and colonization.

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Regulatory interplay of RpoS and RssB controls motility and colonization in Vibrio cholerae

Cited by 7 publications

References 80 publications

DegS protease regulates the motility, chemotaxis, and colonization of Vibrio cholerae

DegS protease regulates the motility, chemotaxis, and colonization of Vibrio cholerae

Chromosomal Integrons are Genetically and Functionally Isolated Units of Genomes

Transcriptomic Analysis of Vibrio parahaemolyticus Underlying the Wrinkly and Smooth Phenotypes

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