Regulatory Interplay of RpoS and RssB Controls Motility and Colonization in  &lt;i&gt;Vibrio cholerae&lt;/i&gt;

Wölflingseder, Martina; Tutz, Sarah; Fengler, Vera H.; Schild, Stefan; Reidl, Joachim

doi:10.2139/ssrn.3979580

Cited by 1 publication

(2 citation statements)

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“…RpoS usually had a positive response to the changes in the external environment or living state, for example, the motility of Vibrio cholerae decreased when the rpoS was deleted (Wölflingseder et al, 2022). However, RpoS also manifested some negative regulations.…”

Section: Rpos Negatively Regulated the Adhesionmentioning

confidence: 99%

“…RpoS is associated with stress adaptation and response to changeable environments (Dodd and Aldsworth, 2002;Dong and Schellhorn, 2010). The function of RpoS has been widely explored in Escherichia coli, Serratia plymuthica, and Vibrio cholerae (Vidovic et al, 2011;Liu et al, 2016;Wölflingseder et al, 2022), and the results showed that RpoS enhanced their adaptation to various environmental stresses (starvation, acidic pH, oxidative stress, etc.). Recent studies found that RpoS can also regulate virulence factor expression, such as adhesion, biofilm-forming, antibiotic tolerance, etc.…”

Section: Introductionmentioning

confidence: 99%

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Sigma factor RpoS positively affects the spoilage activity of Shewanella baltica and negatively regulates its adhesion effect

Zhang

Chen²,

Pan³

et al. 2022

Front. Microbiol.

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Shewanella baltica is the dominant bacterium that causes spoilage of seafood. RpoS is an alternative sigma factor regulating stress adaptation in many bacteria. However, the detailed regulatory mechanism of RpoS in S. baltica remains unclear. This study aims to investigate the regulatory function of RpoS on spoilage activity and adhesion ability in S. baltica. Results revealed that RpoS had no effect on the growth of S. baltica, but positively regulated the spoilage potential of S. baltica accompanied by a slower decline of total volatile basic nitrogen, lightness, and the sensory score of fish fillets inoculated with rpoS mutant. RpoS negatively regulated the adhesion ability, which was manifested in that the bacterial number of rpoS mutant adhered to stainless steel coupon was higher than that of the S. baltica in the early stage, and the biofilm formed on glass slide by rpoS mutant was thicker and tighter compared with S. baltica. Transcriptomic analysis showed that a total of 397 differentially expressed genes were regulated by RpoS. These genes were mainly enrichment in flagellar assembly, fatty acid metabolism/degradation, and RNA degradation pathways, which were associated with motility, biofilm formation and cold adaptation. This study demonstrated that RpoS is a primary regulator involved in flagellar assembly mediated biofilm formation and cold adaptation-related spoilage activity of S. baltica. Our research will provide significant insights into the control of microbiological spoilage in seafood.

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Section: Rpos Negatively Regulated the Adhesionmentioning

confidence: 99%