Regulatory interactions and role in cell type specification of the Malpighian tubules by the cut, Krüppel, and caudal genes of Drosophila

Liu, Su; Jack, Joseph

doi:10.1016/0012-1606(92)90013-7

Cited by 67 publications

(49 citation statements)

References 30 publications

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“…With the beginning of germ band retraction and hindgut formation, Tc-cad expression becomes detectable in the Malpighian tubules (Fig. 4 d and f ), which is again in line with the function of cad in Drosophila (28).…”

Section: Results Tll and Fkh Orthologues From Triboliumsupporting

confidence: 73%

Conserved and divergent aspects of terminal patterning in the beetle Tribolium castaneum

Schröder

Eckert

Wolff

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

114

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To infer similarities and differences in terminal pattern formation in insects, we analyzed several of the key genes of this process in the beetle Tribolium castaneum. We cloned two genes of the terminal pattern cascade, namely tailless (tll) and forkhead ( fkh), from Tribolium and studied their expression patterns. In addition, we analyzed the pattern of MAP kinase activation at blastoderm stage as a possible signature for torso-dependent signaling. Further, we analyzed the late expression of the previously cloned Tribolium caudal (Tc-cad) gene. Finally, we used the upstream region of Tc-tll to drive a reporter gene construct in Drosophila. We find that this construct is activated at the terminal regions in Drosophila, suggesting that the torso-dependent pathway is conserved between the species. We show that most of the expression patterns of the genes studied here are similar in Drosophila and Tribolium, suggesting conserved functions. There is, however, one exception, namely the early function of Tc-tll at the posterior pole. In Drosophila, the posterior tll expression is involved in the direct regulation of the target genes of the terminal pathway. In Tribolium, posterior Tc-tll expression occurs only for a short time and ceases before the target genes known from Drosophila are activated. Thus, we infer that Tc-tll does not function as a direct regulator of segmentation genes at the posterior end. It is more likely to be involved in the early specification of a group of ''terminal'' cells, which begin to differentiate only at a later stage of embryogenesis, when much of the abdominal segmentation process is complete. Thus, there appears to have been a major shift in tll function during the evolutionary transition from short germ to long germ embryogenesis.tailless ͉ forkhead ͉ caudal ͉ torso ͉ short germ insect T erminal pattern formation in Drosophila is maternally initiated at both poles of the egg through the torso-mediated receptor tyrosine kinase pathway (1). Two zygotic gap genes, namely tailless (tll) and huckebein (hkb), are activated in a concentration-dependent manner in response to the signaling activity of torso (tor) (2, 3). Both tll and hkb code for transcription factors that regulate further target genes, among them forkhead ( fkh), brachyenteron (byn), and the terminal hunchback (hb) and wingless (wg) domains (4-8). fkh and wg are also activated by caudal (cad) (8), which is expressed maternally as well as zygotically in the posterior half of the embryo (9, 10). Together these genes are required for patterning the terminal cuticle structures, such as the most posterior segments, the anal pads, and the telson, as well as the stomodaeum, the hindgut, and the Malpighian tubules.Embryogenesis in the flour beetle Tribolium represents a more ancestral form of embryogenesis in insects (11). Nevertheless, it was possible to clone most of the early segmentation genes known from Drosophila also from Tribolium, and the analysis of their expression patterns has suggested that they may have similar functi...

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Section: Results Tll and Fkh Orthologues From Triboliumsupporting

confidence: 73%

Conserved and divergent aspects of terminal patterning in the beetle Tribolium castaneum

Schröder

Eckert

Wolff

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

114

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“…Thus in Kr mutant embryos, cells that remain in the hindgut di¡erentiate some tubule-speci¢c characteristics. Liu & Jack (1992) showed that these cells also express hindgut markers, suggesting that they are partially transformed towards a hindgut fate. However, it is clear that Kr alone is not su¤cient to dictate complete tubule cell di¡erentiation.…”

Section: The Allocation Of Malpighian Tubule Cells and The Formation mentioning

confidence: 99%

“…However, it is clear that Kr alone is not su¤cient to dictate complete tubule cell di¡erentiation. In the absence of Kr, it is tubule eversion that fails and in mutants where Kr expression is reduced, but not lost, the tubules evert but their morphogenesis later in embryogenesis is defective (Harbecke & Janning 1989;Liu & Jack 1992).…”

Section: The Allocation Of Malpighian Tubule Cells and The Formation mentioning

confidence: 99%

Coordinating cell fate and morphogenesis inDrosophilarenal tubules

Ainsworth

Wan

Skaer

2000

Phil. Trans. R. Soc. Lond. B

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Using the renal tubules of Drosophila as an example, we explore how cell speci¢cation leads to the morphogenetic movements that underlie the generation of tissue architecture. Taking two stages of development, we show ¢rst that the tubule cells are allocated by signalling between the endodermal and ectodermal compartments of the posterior gut. Activation of the Wnt pathway patterns the ectodermal anlage, resulting in the expression of tubule genes in a subset of cells and their eversion from the hindgut to form the tubule primordia. We argue that early gene expression directs these morphogenetic movements but not the complete programme of tubule di¡erentiation. In the second example we show that the allocation of the mitogenic tip cell lineage in each tubule is required not only for the normal pattern of cell division but also for the stereotyped three-dimensional arrangement of the mature tubules. Analysis of mutants in which the tip cell lineage is misspeci¢ed reveals that both daughters of the tip cell progenitor are required for the tubules to navigate through the body cavity, so that the distal tips locate in their characteristic positions. We show that the regulator of Rac, Myoblast city, is essential for this second morphogenetic process.

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“…21). Genetic studies in Drosophila suggest that cut plays a role in determining cell-type specificity in several tissues, notably the Malpighian tubules (22)(23)(24)(25)(26)(27)(28)(29)(30)(31). In the mouse, the majority of Cux1 mutant animals die during the perinatal period, whereas about 20% survive to adulthood.…”

mentioning

confidence: 99%

Polycystic Kidneys Caused by Sustained Expression of Cux1 Isoform p75

Cadieux

Harada

Paquet

et al. 2008

Journal of Biological Chemistry

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The transcriptional regulator Cux1 (CDP, Cutl1) is aberrantly expressed in mouse models for polycystic kidney disease. Here we show that p75-Cux1, the shortest isoform of Cux1, transcribed from an alternative promoter within intron 20, is also deregulated in polycystic kidneys derived from Pkd1 mutant embryos. To determine the role of the p75-Cux1 isoform in cystogenesis, we generated transgenic mice expressing p75-CUX1 in the kidneys and other tissues. Strikingly, these animals developed polycystic kidneys at variable penetrance and severity, correlating with transgene expression levels. Histological and marker analysis of p75-CUX1-derived polycystic kidneys revealed renal cysts derived from the tubular nephron, supporting a model of autosomal dominant polycystic kidney disease. Transgenic p75-CUX1 kidneys additionally showed an up-regulation of the protooncogene c-myc and a down-regulation of the cyclin-dependent kinase inhibitor p27. Chromatin affinity purification experiments confirmed the direct interaction of Cux1 with the c-myc and p27 promoters. These molecular alterations were accompanied by an increase in cilia length and in the proliferative index of epithelial cells lining the cysts. Together, these results identify an important role for the short isoform of CUX1 in polycystic kidney disease development.

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Regulatory interactions and role in cell type specification of the Malpighian tubules by the cut, Krüppel, and caudal genes of Drosophila

Cited by 67 publications

References 30 publications

Conserved and divergent aspects of terminal patterning in the beetle Tribolium castaneum

Conserved and divergent aspects of terminal patterning in the beetle Tribolium castaneum

Coordinating cell fate and morphogenesis inDrosophilarenal tubules

Polycystic Kidneys Caused by Sustained Expression of Cux1 Isoform p75

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