Regulatory effects of curcumin on lipid accumulation in monocytes/macrophages

Zingg, Jean-Marc; Hasan, Syeda T.; Cowan, Danica; Ricciarelli, Roberta; Azzi, Angelo; Meydani, Mohsen

doi:10.1002/jcb.23411

Cited by 54 publications

(59 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The p53 and FOXO3a formed part of regulation transcriptional network to control cancer cell growth and apoptosis (33,34). In addition, curcumin induced expression of p53 or/and FOXO3a in inhibition of cancer cell growth and other functions have been shown in other cell systems (27,28,35,36). However, whether curcumin affects the interaction of these proteins to facilitate the inhibitory effect of NPC growth remain to be determined.…”

Section: Discussionmentioning

confidence: 85%

Extracellular signal-regulated kinase signaling-mediated induction and interaction of FOXO3a and p53 contribute to the inhibition of nasopharyngeal carcinoma cell growth by curcumin

Tang

Zhao

et al. 2014

International Journal of Oncology

View full text Add to dashboard Cite

Curcumin, one of the main bioactive components extracted from a traditional Chinese medicinal herb, exhibits potent anticancer activity against many types of cancer cells including nasopharyngeal carcinoma (NPC). However, the detailed molecular mechanism underlying this is not clearly understood. In this study, we showed that curcumin significantly inhibited the growth of NPC cells in a dose-and time-dependent manner as determined by MTT assays, while increasing apoptosis was also observed as measured by flow cytometry for the FITC-Annexin V and propidium iodide (PI) label and Hoechst 33258 staining. To further explore the potential mechanism, we showed that curcumin increased the phosphorylation of ERK1/2 but not p38 MAPK in a time-dependent manner, and induced protein expression of the tumor suppressors FOXO3a and p53 in a dose-dependent manner, which were not observed in the presence of PD98059, an inhibitor of ERK1/2. Furthermore, silencing of FOXO3a and p53 genes by siRNAs overcame the inhibitory effect of curcumin on cell proliferation. Silencing or blockade of p53 using siRNA or chemical inhibitor abrogated the effect of curcumin on expression of FOXO3a protein; silencing or overexpression of FOXO3a had no further effect on curcumin-induced p53 protein expression. Furthermore, blockade of ERK1/2 and exogenous expression of FOXO3a restored the effect of curcumin on growth of cells. Together, our studies show that curcumin inhibits growth and induces apoptosis of NPC cells through ERK1/2-mediated increase in the protein expression and interaction of p53 and FOXO3a. p53 is upstream of FOXO3a, which form a regulatory loop that mediates the effect of curcumin. This study unveils a new mechanism by which curcumin inhibits the proliferation and induces apoptosis of human NPC cells.

show abstract

Section: Discussionmentioning

confidence: 85%

Extracellular signal-regulated kinase signaling-mediated induction and interaction of FOXO3a and p53 contribute to the inhibition of nasopharyngeal carcinoma cell growth by curcumin

Tang

Zhao

et al. 2014

International Journal of Oncology

View full text Add to dashboard Cite

show abstract

“…•Curcumin was more effective than THC in inducing FOXO3a-mediated gene expression by inducing FOXO3a phosphorylation and nuclear translocation [40].…”

Section: Studies Showing Curcumin To Be More Active Than Thcmentioning

confidence: 96%

“…Curcumin has also been shown to affect inflammatory pathways through the modulation of lipid accumulation in monocytes/macrophages [40]. Curcumin increased the expression of two lipid transport genes, the fatty acids transporter CD36/FAT and the fatty acids binding protein 4 (FABP4/aP2), leading to increased lipid levels in cells.…”

Section: Anti-inflammatory Activitiesmentioning

confidence: 99%

Curcumin Differs from Tetrahydrocurcumin for Molecular Targets, Signaling Pathways and Cellular Responses

2014

View full text Add to dashboard Cite

Curcumin (diferuloylmethane), a golden pigment from turmeric, has been linked with antioxidant, anti-inflammatory, anticancer, antiviral, antibacterial, and antidiabetic properties. Most of the these activities have been assigned to methoxy, hydroxyl, α,β-unsaturated carbonyl moiety or to diketone groups present in curcumin. One of the major metabolites of curcumin is tetrahydrocurcumin (THC), which lacks α,β-unsaturated carbonyl moiety and is white in color. Whether THC is superior to curcumin on a molecular level is unclear and thus is the focus of this review. Various studies suggest that curcumin is a more potent antioxidant than THC; curcumin (but not THC) can bind and inhibit numerous targets including DNA (cytosine-5)-methyltransferase-1, heme oxygenase-1, Nrf2, β-catenin, cyclooxygenase-2, NF-kappaB, inducible nitric oxide synthase, nitric oxide, amyloid plaques, reactive oxygen species, vascular endothelial growth factor, cyclin D1, glutathione, P300/CBP, 5-lipoxygenase, cytosolic phospholipase A2, prostaglandin E2, inhibitor of NF-kappaB kinase-1, -2, P38MAPK, p-Tau, tumor necrosis factor-α, forkhead box O3a, CRAC; curcumin can inhibit tumor cell growth and suppress cellular entry of viruses such as influenza A virus and hepatitis C virus much more effectively than THC; curcumin affects membrane mobility; and curcumin is also more effective than THC in suppressing phorbol-ester-induced tumor promotion. Other OPEN ACCESSMolecules 2015, 20 186 studies, however, suggest that THC is superior to curcumin for induction of GSH peroxidase, glutathione-S-transferase, NADPH: quinone reductase, and quenching of free radicals. Most studies have indicated that THC exhibits higher antioxidant activity, but curcumin exhibits both pro-oxidant and antioxidant properties.

show abstract

“…Curcumin has been described as having antioxidant, anti-inflammatory, and anticarcinogenic properties [11–13]. It can also protect against lipid-induced damage in the inflammatory cells of the vascular system by the upregulation of FOXO3a activity [14]. To our knowledge, there has been no report of photodermatitis caused by curcumin.…”

Section: Introductionmentioning

confidence: 99%

The Sonodynamic Effect of Curcumin on THP-1 Cell-Derived Macrophages

Wang

Gao

Guo

et al. 2013

BioMed Research International

View full text Add to dashboard Cite

Curcumin is extracted from the rhizomes of the traditional Chinese herb Curcuma longa and has been proposed to function as a photosensitizer. The potential use of curcumin as a sonosensitizer for sonodynamic therapy (SDT) requires further exploration. This study investigated the sonodynamic effect of curcumin on macrophages, the pivotal inflammatory cells in atherosclerotic plaque. THP-1-derived macrophages were incubated with curcumin at a concentration of 40.7 μmol/L for 2 h and then exposed to pulse ultrasound irradiation (2 W/cm2 with 0.86 MHz) for 5–15 min. Six hours later, cell viability was decreased in cells that had been treated with ultrasound for 10 and 15 min. After ultrasound irradiation for 15 min, the ratio of apoptotic and necrotic cells in SDT group was higher than that in ultrasound group, and the ratio of apoptotic cells was higher than that of necrotic cells. Both loss of mitochondrial membrane potential and morphological changes of cytoskeleton were apparent 2 h after treatment with curcumin SDT. These findings support that curcumin had sonodynamic effect on THP-1-derived macrophages and that curcumin SDT could be a promising treatment for atherosclerosis.

show abstract

Regulatory effects of curcumin on lipid accumulation in monocytes/macrophages

Cited by 54 publications

References 60 publications

Extracellular signal-regulated kinase signaling-mediated induction and interaction of FOXO3a and p53 contribute to the inhibition of nasopharyngeal carcinoma cell growth by curcumin

Extracellular signal-regulated kinase signaling-mediated induction and interaction of FOXO3a and p53 contribute to the inhibition of nasopharyngeal carcinoma cell growth by curcumin

Curcumin Differs from Tetrahydrocurcumin for Molecular Targets, Signaling Pathways and Cellular Responses

The Sonodynamic Effect of Curcumin on THP-1 Cell-Derived Macrophages

Contact Info

Product

Resources

About