Regulatory effect of quercetin on hazardous microcystin-LR-induced apoptosis of Carassius auratus lymphocytes in vitro

Zhang, Hangjun; Wu, Yan; Fang, Wendi; Wang, Dandan

doi:10.1016/j.fsi.2014.02.015

Cited by 30 publications

(17 citation statements)

References 60 publications

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“…In fact, when combined to DDVP (300 μM), QUER (5, 10, and 25 μM) significantly reduced the apoptosis induced by this pesticide by reducing the loss of membrane mitochondrial potential, mitigating DNA fragmentation, and reducing caspase-3 activation. These findings support that QUER induces an antiapoptotic activity as described by Zhang et al (2014) who demonstrated that QUER inhibited microcystin (MCLR)-induced apoptosis in lymphocytes by decreasing the Bax/Bcl-2 ratio.…”

Section: Discussionsupporting

confidence: 89%

Quercetin protects HCT116 cells from Dichlorvos-induced oxidative stress and apoptosis

Salem

Boussabbeh

Graiet

et al. 2016

Cell Stress and Chaperones

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The present study was designed to assess the possible protective effects of Quercetin (QUER), a flavonoid with well-known pharmacological effects, against Dichlorvos (DDVP)-induced toxicity in vitro using HCT116 cells. The cytotoxicity was monitored by cell viability, reactive oxygen species (ROS) generation, anti-oxidant enzyme activities, malondialdehyde (MDA) production, and DNA fragmentation. The apoptosis was assessed through the measurement of the mitochondrial transmembrane potential (ΔΨm) and caspase activation. The results indicated that pretreatment of HCT116 cells with QUER, 2 h prior to DDVP exposure, significantly decreased the DDVP-induced cell death, inhibited the ROS generation, modulated the activities of catalase (CAT) and superoxide dismutase (SOD), and reduced the MDA level. The reductions in mitochondrial membrane potential, DNA fragmentation, and caspase activation were also attenuated by QUER. These findings suggest that dietary QUER can protect HCT116 cells against DDVP-induced oxidative stress and apoptosis.

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Section: Discussionsupporting

confidence: 89%

Quercetin protects HCT116 cells from Dichlorvos-induced oxidative stress and apoptosis

Salem

Boussabbeh

Graiet

et al. 2016

Cell Stress and Chaperones

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“…Therefore, a reduction in the conversion of GSSG back to its reduced form (GSH) occurred following the low levels of GSH. Our previous study indicated that nodularin (100 µg/L) induced oxidative damage of fish lymphocytes via a reduced GSH content and GPx activity (Zhang et al, 2014b).…”

Section: Discussionmentioning

confidence: 96%

Neurotoxic Anatoxin-a Can Also Exert Immunotoxicity by the Induction of Apoptosis on Carassius auratus Lymphocytes in vitro When Exposed to Environmentally Relevant Concentrations

Zhong

Shen

et al. 2020

Front. Physiol.

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“…Mutual interactions between pro-and anti-apoptotic members set the threshold that determines whether a cell survives or dies (Vidal et al 2008). BCL-2, an important antiapoptotic gene, can regulate the initiation and termination of cellular apoptosis mainly by blocking the apoptosis-signaling system (Zhang et al 2014;Hockenbery et al 1990). Numerous researchers identified that BCL-2 could regulate mitochondrial participation in cell death (Li et al 2013;Ouyang and Rona 2014;Langenau et al 2005).…”

Section: Discussionmentioning

confidence: 99%

Identification, characterization and functional analysis of anti-apoptotic protein BCL-2-like gene from pufferfish, Takifugu obscurus, responding to bacterial challenge

Cheng

Liao

Miao

et al. 2015

Fish Physiol Biochem

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Apoptosis plays a crucial role in many biological processes, including development, cellular homeostasis and immune responses. The BCL-2 family is a key regulator of the mitochondrial response to apoptotic signals in the intrinsic pathway. In this study, we identified and characterized the cDNA and expression pattern of pufferfish BCL-2 (PfBCL-2). The full-length cDNA of PfBCL-2 was 1412 bp with an open reading frame of 657 bp encoding a putative protein of 219 amino acids (Accession no: KP898414). The calculated molecular mass of the PfBCL-2 was 24.2 kDa with a predicted isoelectric point of 5.27. The deduced PfBCL-2 protein exhibited four highly conserved BCL-2 homology domains, suggesting that PfBCL-2 may play a similar role in the apoptotic-signaling pathway as in other species. Real-time PCR results showed that PfBCL-2 transcript was expressed in a wide range of tissues but exhibited the greatest level of expression in blood. Transcriptional responses of PfBCL-2 exhibited different spatial and temporal expression profiles in liver and blood after bacterial infection. PfBcl-2 transcript was significantly up-regulated in liver at 6, 12, 24 and 48 h (with maximum induction at 48 h) and was up-regulated in blood at 3, 6, 12 and 24 h (with maximum induction at 12 h). Meanwhile, recombinant PfBCL-2 fused with His6 tag was efficiently expressed in Escherichia coli BL21 (DE3) and purified using Ni-nitrilotriacetic acid resin. Western blot analysis indicated that its protein level appeared to be elevated during the initial bacterial infection. These results suggest that PfBCL-2 plays important roles in immune responses against bacteria challenge.

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Regulatory effect of quercetin on hazardous microcystin-LR-induced apoptosis of Carassius auratus lymphocytes in vitro

Cited by 30 publications

References 60 publications

Quercetin protects HCT116 cells from Dichlorvos-induced oxidative stress and apoptosis

Quercetin protects HCT116 cells from Dichlorvos-induced oxidative stress and apoptosis

Neurotoxic Anatoxin-a Can Also Exert Immunotoxicity by the Induction of Apoptosis on Carassius auratus Lymphocytes in vitro When Exposed to Environmentally Relevant Concentrations

Identification, characterization and functional analysis of anti-apoptotic protein BCL-2-like gene from pufferfish, Takifugu obscurus, responding to bacterial challenge

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