Regulatory crosstalk at composite response elements

Miner, Jeffrey N.

doi:10.1016/0968-0004(91)90168-u

Cited by 243 publications

(116 citation statements)

References 27 publications

Supporting

Mentioning

110

Contrasting

Unclassified

Order By: Relevance

“…These elements include binding sites for non-GR regulators that participate in a GRE-specific manner in the hormone response. At some composite GREs, such as that for the rat phosphoenolpyruvate carboxykinase (PEPCK) gene, the nonreceptor regulators are required for GR recruitment to its DNA-binding sites (Stafford et al 2001); at others, such as that for the proliferin gene, GR binds to the GRE independently but depends on nonreceptor regulators to define its activity (Diamond et al 1990;Miner and Yamamoto 1991). Furthermore, GR also can act from "tethering" GREs, at which the receptor fails to bind DNA, and instead associates through protein-protein interaction with a DNA-bound nonreceptor regulator (Yamamoto 1995;Luecke and Yamamoto 2005).…”

Section: Discussionmentioning

confidence: 99%

Novel arylpyrazole compounds selectively modulate glucocorticoid receptor regulatory activity

Wang¹,

Shah²,

Pantoja³

et al. 2006

Genes Dev.

Self Cite

View full text Add to dashboard Cite

The activities of intracellular receptors are regulated by their cognate ligands. Here we show that a series of related arylpyrazole compounds, which specifically bind the glucocorticoid receptor (GR), selectively modulated GR-regulated biological functions in preadipocyte, preosteoblast, and lung epithelial cell lines. Indeed, when we monitored 17 endogenous GR target genes in one of these cell types, we found that distinct arylpyrazole compounds induced different expression patterns. We showed by chromatin immunoprecipitation that the arylpyrazole compounds regulated, in a gene-specific manner, either GR occupancy of the genomic glucocorticoid response element (GRE) or events after GR association, such as histone modification. Overall, our results establish that subtle differences in ligand chemistry can profoundly influence the transcriptional regulatory activity of GR, and that endogenous genes bearing natural GREs are especially sensitive detectors of these differences.[Keywords: Glucocorticoid receptor; glucocorticoid receptor ligand; arylpyrazole compounds; response element; chromatin immunoprecipitation] Supplemental material is available at http://www.genesdev.org.

show abstract

Section: Discussionmentioning

confidence: 99%

Novel arylpyrazole compounds selectively modulate glucocorticoid receptor regulatory activity

Wang¹,

Shah²,

Pantoja³

et al. 2006

Genes Dev.

Self Cite

View full text Add to dashboard Cite

show abstract

“…This indicates that VP1 has a function in repression that is mechanistically distinct from the transcriptional activation function. Several systems in which a single transcription factor functions as both an activator and a repressor depending on the promoter context have been described in animals (Miner and Yamamoto 1991 ;Tsai and O'Malley 1994). Direct structural homologs of VP1 are thus far known only in plants, suggesting that this strategy has evolved independently in plants and animals.…”

Section: Gene Repression Is Distinct From Activation Functions Of Vplmentioning

confidence: 99%

Integrated control of seed maturation and germination programs by activator and repressor functions of Viviparous-1 of maize.

Hoecker¹,

Vasil²,

McCarty³

1995

Genes Dev.

202

160

View full text Add to dashboard Cite

The Viviparous-1 (VP1) transcriptional activator of maize is required for abscisic acid induction of maturation-specific genes late in seed development leading to acquisition of desiccation tolerance and arrest in embryo growth. Here, we show that VP1 also inhibits induction of the germination-specific a-amylase genes in aleurone cells of the developing seed and thereby appears to be involved in preventing precocious hydrolyzation of storage compounds accumulating in the endosperm. In developing seeds of the somatically instable vpl-m2 mutant, hydrolase activity was derepressed specifically in endosperm sectors underlying vpl mutant aleurone. A barley a-amylase promoter-GUS reporter construct (Amy-GUS) was induced in developing vpl mutant aleurone cells but not in wild-type aleurone cells. Moreover, transient expression of recombinant VP1 in vpl mutant aleurone cells strongly inhibited expression of Amy-GUS and thus effectively complemented this aspect of the mutant phenotype. VP1 specifically repressed induction of Amy-GUS by the hormone gibberellic acid in aleurone of germinating barley seeds. Deletion of the acidic transcriptional activation domain of VP1 did not affect the inhibitory activity, indicating that VP1 has a discrete repressor function. Hence, physically combining activator and repressor functions in one protein may provide a mechanism to integrate the control of two normally consecutive developmental programs, seed maturation and seed germination.

show abstract

“…2) for the overlapping binding site. 23,24 The weak DISCUSSION inhibition of the OATP promoter by glucocorticoids could be secondary to the low level of glucocorticoid receptor The present study reports the genomic cloning of a sodium-independent multispecific transport protein of the ba-expression in HepG2 cells, as has been reported for the GREcontaining promoter of the human cholesterol 7a-hydroxysolateral hepatocyte membrane, the human OATP gene, as well as initial data on the function and regulation of the lase gene (CYP7A.) 19 Significant inhibition of the CYP7A promoter by glucocorticoids was only observed when HepG2 OATP promoter.…”

Section: Isolation Of the 5 -Region Of The Oatp Cdna And Primer Extenmentioning

confidence: 99%

Identification and functional characterization of the promoter region of the human organic anion transporting polypeptide gene

Kullak-Ublick¹

1997

Hepatology

View full text Add to dashboard Cite

The liver extracts a large variety of organic anions and The organic anion transporting polypeptide (OATP) of the amphipathic compounds from the sinusoidal blood by carbasolateral hepatocyte membrane mediates multispecific uprier-mediated transport across the basolateral hepatocyte take of anionic and other amphipathic substrates from sinumembrane. Expression cloning strategies in Xenopus laevis soidal blood plasma. To investigate the mechanisms controloocytes have led to the isolation of a sodium-bile acid coling OATP expression, the 5 -flanking region of the human transport system (Na / -taurocholate cotransporting polypep-OATP gene was isolated from a P1-derived artificial chromotide) as well as of a sodium-independent organic anion transsome genomic clone. Sequence analysis of the OATP promoter porting polypeptide (oatp) from rat and human liver. 1 The showed a number of consensus binding sites for both ubiquihuman OATP is a multispecific and polyvalent amphipathic tous and liver-enriched transcription factors. Transfection of substrate transporter that can mediate hepatocellular uptake HepG2 cells with a series of 5 -deleted promoter-luciferase of organic anions, such as bile acids, bromosulphophthalein, constructs identified the minimal promoter region within 91 estrone-3-sulfate, estradiol-17-glucuronide, the cardiac glybase pairs relative to the transcription initiation site. A puta-coside ouabain, and even the cationic compound N-(4,4-tive silencer element was localized in the 0662/0440 region. azo-n-pentyl)-21-deoxy-ajmalinium. 2,3 OATP consists of 670 The minimal promoter was also active in Chang liver, Madin-amino acids with 12 putative membrane-spanning domains Darby canine kidney, and Chinese hamster ovary cells, indi-and seven potential N-linked glycosylation sites. Based on cating that basal promoter function is independent of liver-Northern blot analysis, related proteins are also expressed in specific regulatory mechanisms. In transfected HepG2 cells, other tissues, including kidney and brain. 2 The OATP gene taurocholate (100 mmol/L) stimulated and triiodothyronine has been localized to chromosome 12p12. 4 (1 mmol/L) inhibited OATP promoter activity, whereas hydroThe factors that control the expression of OATP in normal cortisone, dexamethasone, b-estradiol, estrone-3-sulfate, and and diseased liver are as yet unknown. As a first step towards testosterone had no significant effect. Reverse-transcription understanding the regulation of OATP expression, our aim polymerase chain reaction analysis showed an increase in was to isolate the 5 -flanking region of the OATP gene and OATP messenger RNA in the livers of four patients with to investigate the response of the OATP promoter to various chronic cholestatic liver disease compared with three noncho-physiological and pathophysiological stimuli. The data prelestatic controls. The up-regulation of OATP expression by sented in this study indicate that the OATP-mediated uptake taurocholate could serve to enhance the sinusoidal efflux of of cholephilic...

show abstract

Regulatory crosstalk at composite response elements

Cited by 243 publications

References 27 publications

Novel arylpyrazole compounds selectively modulate glucocorticoid receptor regulatory activity

Novel arylpyrazole compounds selectively modulate glucocorticoid receptor regulatory activity

Integrated control of seed maturation and germination programs by activator and repressor functions of Viviparous-1 of maize.

Identification and functional characterization of the promoter region of the human organic anion transporting polypeptide gene

Contact Info

Product

Resources

About