Regulator ThnR and the ThnDE ABC transporter proteins confer autoimmunity to thurincin H in Bacillus thuringiensis

Casados‐Vázquez, Luz E.; Bideshi, Dennis K.; Barboza‐Corona, José E.

doi:10.1007/s10482-018-1124-7

Cited by 11 publications

(7 citation statements)

References 49 publications

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“…Moreover, some native transporters are substrate‐inducible, which prevents membrane burden caused by transporter overexpression in unnecessary conditions. [ 114 ] Based on this phenomenon, substrate‐responsive natural transporter promoters were discovered and related transcription factors were designed, which have been utilized to develop biosensors for high‐throughput screening of high‐producing strains. [ 115 ] These elements were even found to have the ability of a feedback regulation in native transporter expression.…”

Section: Resultsmentioning

confidence: 99%

Transporter Engineering for Microbial Manufacturing

Zhu

Zhou

Wang

et al. 2020

Biotechnology Journal

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Microbes play an important role in biotransformation and biosynthesis of biofuels, natural products, and polymers. Therefore, microbial manufacturing has been widely used in medicine, industry, and agriculture. However, common strategies including enzyme engineering, pathway optimization, and host engineering are generally inadequate to obtain an efficient microbial production system. Transporter engineering provides an alternative strategy to promote the transmembrane transfer of substrates, intermediates, and final products in microbial cells and thus enhances production by alleviating feedback inhibition and cytotoxicity caused by final products. According to the current studies in transport engineering, native transporters usually have low expression and poor transportation ability, resulting in inefficient transport processes and microbial production. In this review, current approaches for transporter mining, characterization, and verification are comprehensively summarized. Practical approaches to enhance the transport system in engineered cells, such as balancing transporter overexpression and cell growth, and evolution of native transporters are discussed. Furthermore, the applications of transporter engineering in microbial manufacturing, including enhancement of substrate utilization, concentration of metabolic flux to the target pathway, and acceleration of efflux and recovery of products, demonstrate its outstanding advantages and promising prospects.

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Section: Resultsmentioning

confidence: 99%

Transporter Engineering for Microbial Manufacturing

Zhu

Zhou

Wang

et al. 2020

Biotechnology Journal

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“…Although the antimicrobial activity was low (~4000 U mg −1 ) at 72 h, it was sufficient to increase the germination percentage and root length. The thnR , theD and thnE genes are responsible for thurincin H immunity, this gene cluster is regulated by their native promoter (Casados‐Vázquez et al, 2018). The activation mechanism of the native promoter has not been established, but it needs to be activated in the presence of thurincin H to express the immunity genes, since expression of thnA , thnB , thnT and thnI genes did not result in thurincin H production (Casados‐Vázquez et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…One of the most studied is thurincin H, which is an antimicrobial peptide of 31 amino acids with the potential to be used as a growth factor (Lee, Churey, et al, 2009; Lee, Gray, et al, 2009). The synthesis, posttranslational modification, and translocation of thurincin H are carried out by proteins encoded by a cluster of ten genes whose expression is controlled by a bidirectional promoter (Casados‐Vázquez et al, 2018; Oros‐Flores et al, 2018). Thurincin H can be used as a growth factor, and its application to leaves or roots has stimulated growth in soybean and corn.…”

Section: Introductionmentioning

confidence: 99%

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Expression of thurincin H, ChiA74 and Cry proteins at the sporulation phase in Bacillus thuringiensis HD1

Rivas

Barboza‐Corona

Massange-Sánchez

et al. 2022

Journal of Applied Microbiology

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Aims:The objective of this study was to produce thurincin H, ChiA74 and Cry proteins together using Bacillus thuringiensis subsp. kurstaki HD1 as a heterologous host. Methods and Results: pSTAB-ThurH and pSTAB-ChiA74 constructs were designed to produce thurincin H and chitinase, respectively, at the sporulation phase.They were transformed into Bt HD1 generating the recombinant strains HD1/ pSTAB-ThurH and HD1/pSTAB-ThurH/pSTAB-ChiA74. Antimicrobial and chitinolytic activity tests were performed with recombinant strains. Both strains were able to produce thurincin H up to 72 h with antibacterial activity of ~4000 U mg −1 .The HD1/pSTAB-ThurH/pSTAB-ChiA74 strain also showed chitinolytic activity of ~23 mU mg −1 at 72 h. All B. thuringiensis strains exhibited crystal formation at 72, and 96 h. In addition, the application of thurincin H in corn seeds increased the germination percentage and root length by 7% and 10%, respectively. Conclusions:We showed that is possible to produce three proteins of biotechnological interest at the sporulation stage in B. thuringiensis, which two of them (thurincin H, and ChiA74) are naturally expressed in the vegetative stage.Significance and Impact of the Study: These results form the basis for developing of a biocontrol and biostimulator product that can be used as an alternative for chemical application.

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“…Chitinolytic bacteria produces a number of chitinolytic enzymes that convert chitin into carbon and nitrogen sources, which in combination with other metabolites, help the plants to grow. In recent times, the chitinase produced by Bt have been widely studied due to its potential applications not only in insect control but also as antibacterial and fungicidal specialist (Azizoglu, 2019;Casados-Vazquez et al, 2018;Jouzani et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of chitinase producing Bacillus thuringiensis

Singh¹,

Joshi²,

Nath³

et al. 2022

JEB

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Aim: In the present study, twenty eight Bacillus thuringiensis (Bt) strains were studied for their potential to hydrolyze chitin. Methodology: The chitinolytic potential of Bt strains were determined using modified chitinase screening media (CSM) containing colloidal chitin as substrate. The potential Bt strains were screened for the presence of cry genes using PCR and further molecular characterized using 16S rDNA amplification and sequencing. Results: Among 28 Bt strains, 10 strains showed positive results by producing clear halo zone around bacterial colonies and the maximum chitinase solubilization index was observed in Bt-13 whereas the minimum solubilization index was observed in Bt-27 strain. Further, the chitinase assay revealed the minimum enzyme activity in Bt-26 (3.78 ± 0.101 U ml-1) whereas the maximum activity was observed in strain Bt-2 (10.19 ± 0.651 U ml-1). PCR based chitinase gene screening also revealed the presence of both endochitinase and exochitinase genes in these Bt strains. The potent Bt strains viz., Bt-7, Bt-10, Bt-11, Bt-13 and Bt-27 on the basis of chitinase production were molecular characterized based on 16S rDNA and sequencing results reveled their greatest sequence identity Bacillus thuringiensis. Interpretation: Results showed that the high chitinase activity of these Bt strains may be due to the presence of chitinase genes, which need to be explored for further biological applications.

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Regulator ThnR and the ThnDE ABC transporter proteins confer autoimmunity to thurincin H in Bacillus thuringiensis

Cited by 11 publications

References 49 publications

Transporter Engineering for Microbial Manufacturing

Transporter Engineering for Microbial Manufacturing

Expression of thurincin H, ChiA74 and Cry proteins at the sporulation phase in Bacillus thuringiensis HD1

Molecular characterization of chitinase producing Bacillus thuringiensis

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