Regulator of G protein signaling-1 regulates immune infiltration and macrophage polarization in clear cell renal cell carcinoma

Abstract

Objective: To better understand how to clear cell renal cell cancer (ccRCC) is affected by the regulator of G protein signaling-1 (RGS1), its effect on immune infiltration, macrophage polarization, tumor proliferation migration. Patients and Methods: In this study, a total of 20 surgical specimens of patients with pathological diagnosis of ccRCC were selected for testing, while the expression of RGS1 in tumors, immune infiltration, macrophage polarization, were combined with TGCA database and GO analysis. We also further explored and studied the expression and function of RGS1 in tumor microenvironment(TME), investigated how RGS1 affected tumor growth, migration, apoptosis, and other traits. Results: RGS1 was found to be expressed at higher quantities in ccRCC than in normal cells or tissues. By using the TCGA database and GO analysis to describe the expression of RGS1 in a range of tumors, it was found that ccRCC had a much higher level of RGS1 expression than other tumor types. The results of gene enrichment analysis indicated that overexpression of RGS1 may be associated with immune infiltration. The outcomes of in vitro tests revealed that RGS1 overexpression in ccRCC did not significantly alter the proliferation and migration ability of ccRCC, but RGS1 overexpression promoted apoptosis in ccRCC. By in vitro co-culture experiments, RGS1 overexpression inhibited M2 macrophage polarization and also suppressed the Jagged-1/Notch signaling pathway. Conclusions: RGS1 is highly expressed in ccRCC, while overexpression of RGS1 may increase immune infiltration in the TME and reduce the polarization of M2 macrophages while promoting apoptosis in ccRCC.