Regulations on growth and development in tomato cotyledon, flower and fruit via destruction of miR396 with short tandem target mimic

Cao, Deliang; Wang, Jiao; Zheng, Jie; Liu, Qingqing; Li, Shan; Tian, Huiqin; Fu, Daqi; Zhu, Hongliang; Luo, Yunbo; Zhu, Benzhong

doi:10.1016/j.plantsci.2016.02.012

Cited by 93 publications

(76 citation statements)

References 48 publications

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“…Recent study showed that miR396 modulates fruit enlargement and weight via GRF transcription factors in tomato [15]. In this study, seven GRF transcription factors were predicted to be targets of cme-miR396 (Table 3, S7 Table).…”

Section: Discussionmentioning

confidence: 72%

“…miR156-SPL module is involved in meristem maintenance in the developing ovaries of tomato, controlling initial steps of fleshy fruit development and determinacy [14]. miR396 negatively regulates the fruit size and weight of tomato fruit [15]. In recent years, high-throughput sequencing has been used for the identification and expression profiling of miRNAs in many fruit crops, such as tomato [16–19], melon [20], apple [21], grape [22], strawberry [23], papaya [24], sweet orange [25], and pear [26].…”

Section: Introductionmentioning

confidence: 99%

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Genome-wide identification of Hami melon miRNAs with putative roles during fruit development

Zhang

Yin²,

Wang

et al. 2017

PLoS ONE

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MicroRNAs represent a family of small endogenous, non-coding RNAs that play critical regulatory roles in plant growth, development, and environmental stress responses. Hami melon is famous for its attractive flavor and excellent nutritional value, however, the mechanisms underlying the fruit development and ripening remains largely unknown. Here, we performed small RNA sequencing to investigate the roles of miRNAs during Hami melon fruit development. Two batches of flesh samples were collected at four fruit development stages. Small RNA sequencing yielded a total of 54,553,424 raw reads from eight libraries. 113 conserved miRNAs belonging to 30 miRNA families and nine novel miRNAs comprising nine miRNA families were identified. The expression of 42 conserved miRNAs and three Hami melon-specific miRNAs significantly changed during fruit development. Furthermore, 484 and 124 melon genes were predicted as putative targets of 29 conserved and nine Hami melon-specific miRNA families, respectively. GO enrichment analysis were performed on target genes, "transcription, DNA-dependent", "rRNA processing", "oxidation reduction", "signal transduction", "regulation of transcription, DNA-dependent", and "metabolic process" were the over-represented biological process terms. Cleavage sites of six target genes were validated using 5' RACE. Our results present a comprehensive set of identification and characterization of Hami melon fruit miRNAs and their potential targets, which provide valuable basis towards understanding the regulatory mechanisms in programmed process of normal Hami fruit development and ripening. Specific miRNAs could be selected for further research and applications in breeding practices.

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Section: Discussionmentioning

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Genome-wide identification of Hami melon miRNAs with putative roles during fruit development

Zhang

Yin²,

Wang

et al. 2017

PLoS ONE

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“…Similarly, os-miR396c over-expression decreased rice grain yield, but the synonymous mutation of OsGRF4 (i.e., disruption of the os-miR396c target) enhanced grain yield (Li et al, 2016). Baucher et al (2013) and Cao et al (2016) showed that miR396 targets GRFs to control floral organ development, and Liang et al (2014) reported that miR396 regulates GRF to control carpel number and pistil development.…”

Section: Discussionmentioning

confidence: 99%

Tissue-Specific Regulation of Gma-miR396 Family on Coordinating Development and Low Water Availability Responses

Liu

Zhou

et al. 2017

Front. Plant Sci.

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Previously, it was reported that miR396s interact with growth-regulating factors (GRFs) to modulate plant growth, development, and stress resistance. In soybean, 11 gma-miR396 precursors (Pre-miR396a–k) were found, and 24 GmGRFs were predicted as targets of seven mature gma-miR396s (gma-miR396a/b/c/e/h/i/k). To explore the roles of the miR396–GRF module in low water availability response of soybean, we analyzed the expression of Pre-miR396a–k, and found that Pre-miR396a/i/bdgk/e/h were up-regulated in leaves and down-regulated in roots; on the contrary, GmGRF5/6/7/8/15/17/21 were down-regulated in leaves and GmGRF1/2/17/18/19/20/21/22/23/24 were up-regulated in roots of low water potential stressed soybean. Any one of gma-miR396a/b/c/e/h/i/k was able to interact with 20 GmGRFs (GmGRF1/2/6–11/13–24), confirming that this module represents a multi-to-multi network interaction. We generated Arabidopsis plants over-expressing each of the 11 gma-miR396 precursors (Pre-miR396a–k), and seven of them (miR396a/b/c/e/h/i/k-OE transgenic Arabidopsis) showed altered development. The low water availability of miR396a/b/c/e/h/i/k-OE was enhanced in leaves but reduced in seeds and roots. Contrary to previous reports, miR396a/b/c/i-OE seedlings showed lower survival rate than WT when recovering after rewatering under soil drying. In general, we believe our findings are valuable to understand the role of gma-miR396 family in coordinating development and low water availability responses, and can provide potential strategies and directions for soybean breeding programs to improve seed yield and plant drought tolerance.

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“…In addition to the N-terminal conserved QLQ and WRC domains reported by Cao et al [24], the SlGRF proteins further contain C-terminal amino acid motifs such as TQL, FFD similar to Arabidopsis GRF proteins [8]. These C-terminal motifs have significant role in the GRF proteins function in different plant tissues and organs [26].…”

Section: Discussionmentioning

confidence: 99%

“…Cao et al (2016) reported approximately 13 GRF family genes in tomato but their involvement in growth, development and defense-related activities was not extensively studied in tomato [24]. Here, we systematically characterized all 13 putative GRF family genes of tomato and carried out an evolutionary analysis of tomato GRF genes by comparing them with those of Arabidopsis , rice, maize and Chinese cabbage.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization and Expression Profiling of Tomato GRF Transcription Factor Family Genes in Response to Abiotic Stresses and Phytohormones

Khatun

Robin

Park

et al. 2017

IJMS

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Growth regulating factors (GRFs) are plant-specific transcription factors that are involved in diverse biological and physiological processes, such as growth, development and stress and hormone responses. However, the roles of GRFs in vegetative and reproductive growth, development and stress responses in tomato (Solanum lycopersicum) have not been extensively explored. In this study, we characterized the 13 SlGRF genes. In silico analysis of protein motif organization, intron–exon distribution, and phylogenetic classification confirmed the presence of GRF proteins in tomato. The tissue-specific expression analysis revealed that most of the SlGRF genes were preferentially expressed in young and growing tissues such as flower buds and meristems, suggesting that SlGRFs are important during growth and development of these tissues. Some of the SlGRF genes were preferentially expressed in fruits at distinct developmental stages suggesting their involvement in fruit development and the ripening process. The strong and differential expression of different SlGRFs under NaCl, drought, heat, cold, abscisic acid (ABA), and jasmonic acid (JA) treatment, predict possible functions for these genes in stress responses in addition to their growth regulatory functions. Further, differential expression of SlGRF genes upon gibberellic acid (GA3) treatment indicates their probable function in flower development and stress responses through a gibberellic acid (GA)-mediated pathway. The results of this study provide a basis for further functional analysis and characterization of this important gene family in tomato.

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Regulations on growth and development in tomato cotyledon, flower and fruit via destruction of miR396 with short tandem target mimic

Cited by 93 publications

References 48 publications

Genome-wide identification of Hami melon miRNAs with putative roles during fruit development

Genome-wide identification of Hami melon miRNAs with putative roles during fruit development

Tissue-Specific Regulation of Gma-miR396 Family on Coordinating Development and Low Water Availability Responses

Molecular Characterization and Expression Profiling of Tomato GRF Transcription Factor Family Genes in Response to Abiotic Stresses and Phytohormones

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