Regulation of β-1,3-glucanase activity in developing anthers of Lilium

Stieglitz, Heather; Stern, Herbert

doi:10.1016/0012-1606(73)90347-3

Cited by 82 publications

(55 citation statements)

References 13 publications

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“…After the tetrad stage, callase digests the callose both at the exterior of the tetrad and between the microspores to release and separate individual microspores (Stieglitz and Stern, 1973;Scott et al, 2004). The accurate timing of callase activation is critical for normal microsporogenesis and pollen development.…”

Section: Discussion Cdm1 Likely Regulates Expression Of Genes For Botmentioning

confidence: 99%

“…6, D and E) suggested that they potentially function at different anther stages or at different steps of callose dissolution, possibly even in different subcellular compartments. The callase mixture includes endoglucanases and exoglucanases (Stieglitz and Stern, 1973;Scott et al, 2004). Endoglucanases cleave b-1,3-glucans into short-chain reducing sugars; exoglucanase hydrolysis releases a single Glc unit from the reducing ends of the substrate (Stieglitz, 1977;Zhang et al, 2007).…”

Section: Callose Dissolution During Pollen Development Is a Highly Comentioning

confidence: 99%

“…Callase is considered to be secreted by tapetum to separate newly formed microspores (Stieglitz and Stern, 1973;Stieglitz, 1977;Scott et al, 2004). However, recent RNA sequencing data from Arabidopsis male meiocytes detected several b-1,3-G genes (At3g55430, At2g01730, and At5g20390) expressed in these reproductive cells , suggesting that male meiocytes probably also synthesize b-1,3-Gs.…”

Section: Callose Dissolution During Pollen Development Is a Highly Comentioning

confidence: 99%

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The Arabidopsis CALLOSE DEFECTIVE MICROSPORE1 Gene Is Required for Male Fertility through Regulating Callose Metabolism during Microsporogenesis

Chai

Yang

et al. 2014

Plant Physiol.

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ORCID IDs: 0000-0002-3941-9955 (P.L.); 0000-0001-8717-4422 (H.M.).During angiosperm microsporogenesis, callose serves as a temporary wall to separate microsporocytes and newly formed microspores in the tetrad. Abnormal callose deposition and dissolution can lead to degeneration of developing microspores. However, genes and their regulation in callose metabolism during microsporogenesis still remain largely unclear. Here, we demonstrated that the Arabidopsis (Arabidopsis thaliana) CALLOSE DEFECTIVE MICROSPORE1 (CDM1) gene, encoding a tandem CCCH-type zinc finger protein, plays an important role in regulation of callose metabolism in male meiocytes and in integrity of newly formed microspores. First, quantitative reverse transcription PCR and in situ hybridization analyses showed that the CDM1 gene was highly expressed in meiocytes and the tapetum from anther stages 4 to 7. In addition, a transfer DNA insertional cdm1 mutant was completely male sterile. Moreover, light microscopy of anther sections revealed that microspores in the mutant anther were initiated, and then degenerated soon afterward with callose deposition defects, eventually leading to male sterility. Furthermore, transmission electron microscopy demonstrated that pollen exine formation was severely affected in the cdm1 mutant. Finally, we found that the cdm1 mutation affected the expression of callose synthesis genes (CALLOSE SYNTHASE5 and CALLOSE SYNTHASE12) and potential callase-related genes (A6 and MYB80), as well as three other putative b-1,3-glucanase genes. Therefore, we propose that the CDM1 gene regulates callose metabolism during microsporogenesis, thereby promoting Arabidopsis male fertility.

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Section: Discussion Cdm1 Likely Regulates Expression Of Genes For Botmentioning

confidence: 99%

Section: Callose Dissolution During Pollen Development Is a Highly Comentioning

confidence: 99%

Section: Callose Dissolution During Pollen Development Is a Highly Comentioning

confidence: 99%

See 1 more Smart Citation

The Arabidopsis CALLOSE DEFECTIVE MICROSPORE1 Gene Is Required for Male Fertility through Regulating Callose Metabolism during Microsporogenesis

Chai

Yang

et al. 2014

Plant Physiol.

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“…In the anthers of petunia and lily, the expression and secretion of callase activity is under strict developmental control (Frankel et al, 1969;Stieglitz and Stern, 1973). Alterations in the timing of b-1,3-glucanase expression, or failure to express b-1,3-glucanase, leads to abnormal dissolution of the tetrad callose walls, which has been shown to be a primary cause of male sterility in cytoplasmic male-sterile lines of several species, including petunia (Izhar and Frankel, 1971).…”

Section: The Cell Wall In Male Meiosismentioning

confidence: 99%

Stamen Structure and Function

Scott

Spielman

Dickinson

2004

THE PLANT CELL ONLINE

576

534

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“…3) At the end of meiosis, tetrads of haploid microspores are freed into the locule by the action of a -1,3-glucanase (callase) which is secreted by the tapetal cells. 4) In transgenic tobacco plants in which -1,3-glucanase is induced prematurely, little fertile pollen is produced. 5) Therefore, the timing of -1,3-glucanase activity is essential for the developing microspores.…”

mentioning

confidence: 99%