Regulation of wheat bran feruloyl oligosaccharides in the intestinal antioxidative capacity of rats associated with the p38/JNK‐Nrf2 signaling pathway and gut microbiota

Abstract

BACKGROUND Feruloyl oligosaccharides (FOs), the ferulic acid ester of oligosaccharides, may possess the physiological functions of both ferulic acid and oligosaccharides, including antioxidative activity and gut microbiota modulation capacity. The present study aimed to investigate whether FOs could regulate the intestinal antioxidative capacity of rats by modulating the MAPKs/Nrf2 signaling pathway and gut microbiota. Thirty Wistar rats were randomly divided into five groups. Rats received a standard diet and were gavaged once daily with 0.85% normal saline, 100 mg kg−1 body weight vitamin C or FOs solution at doses of 20, 40 and 80 mg kg−1 body weight for 21 days. RESULTS FOs strengthened the antioxidative capacity of the jejunum, as indicated by increased in contents of catalase, superoxide dismutase and glutathione peroxidase, as well as glutathione. Moreover, FOs administration upregulated the mRNA expression level of antioxidant‐related genes (glutamate‐cysteine ligase catalytic subunit, glutamate‐cysteine ligase modifier subunit and heme oxygenase‐1) in the jejunum. Increases in phosphorylation levels of Nrf2, p38 and JNK were also observed. Administration with 40 mg kg−1 FOs altered the structure and composition of the cecal microbiota, which was indicated by the increased the relative abundances of Actinobacteria, Proteobacteria and Acidobacteriota, and the decreased the relative abundances of Firmicutes, Lachnospiraceae_NK4A136_group and Blautia. Furthermore, Spearman correlation analysis revealed that the altered cecal microbiota closely correlated with jejunal antioxidative capacity of rats. CONCLUSION FOs could be used as an antioxidant for gut heath improvement through modulating the p38/JNK‐Nrf2 signaling pathway and gut microbiota. © 2022 Society of Chemical Industry.

Dietary Catalase Supplementation Alleviates Deoxynivalenol-Induced Oxidative Stress and Gut Microbiota Dysbiosis in Broiler Chickens

“…In contrast to the DON group, the DONC group was differentially enriched with several potentially beneficial bacteria, such as the phylum Acidobacteriota, along with the genera Anaerofustis and Anaerotruncus. It was indicated that Acidobacteriota might favor the suppression of intestinal oxidative stress, due to its connection with intestinal anti-inflammation and the antioxidation of animals [50,51]. Likewise, this study disclosed a positive correlation of the abundance of Acidobacteriota with intestinal T-AOC of broilers.…”

Ferulic Acid as a Protective Antioxidant of Human Intestinal Epithelial Cells

“…Feruloyl Oligosaccharides (FOs), the ferulic acid ester of oligosaccharides, has been reported to strengthen the antioxidative capacity of the jejunum and alter the structure and composition of the cecal microbiota in rats by increasing the relative abundances of Actinobacteria, Proteobacteria while decreasing the abundance of Firmicutes. [ 75 ]. In the future, it would be interesting to follow the gut microbiome-modulating effect of FA on gut health.…”

Synthesis and Evaluation of Antioxidant and Potential Prebiotic Activities of Acetylated and Butyrylated Fructo-Oligosaccharides

“…Numerous modifications of polysaccharides including sulfation, phosphorylation, carboxymethylation, and selenization can boost antioxidant activities and may be effective in securing food, pharmaceutical, and cosmetic formulations. Feruloyl oligosaccharides strengthened the antioxidative capacity of the jejunum, as evidenced by increased contents of catalase, superoxide dismutase, glutathione peroxidase, and glutathione [ 38 ]. Although no differences in antioxidant activities were observed between FOS and Ac-FOS, excellent antioxidant activities were found when FOS was butyrylated.…”