Regulation of voltage-gated sodium channel expression in cancer: hormones, growth factors and auto-regulation

Fraser, Scott P.; Ozerlat-Gunduz, Iley; Brackenbury, William J.; FitzGerald, Elizabeth; Campbell, Thomas M.; Coombes, R. Charles; Djamgoz, Mustafa B.A.

doi:10.1098/rstb.2013.0105

Cited by 124 publications

(113 citation statements)

References 115 publications

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“…Besides, 31 deregulated coding transcripts target the wound healing process. Growth evidence shows that voltage-gated channel activity alteration of carcinoma cell membrane is a characteristic of carcinoma cells (29)(30)(31). In our results, the molecules target voltage-gated ion channel activity, voltage-gated channel activity, voltage-gated cation We constructed a coding-non-coding gene co-expression network (CNC), by analyzing the correlation of lncRNAs and the expression level of the coding gene transcripts.…”

Section: Discussionmentioning

confidence: 77%

Microarray expression profile of lncRNAs and the upregulated ASLNC04080 lncRNA in human endometrial carcinoma

Zhai

et al. 2015

International Journal of Oncology

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Abstract. Long non-coding RNAs (lncRNAs) have been recognized as a regulator of gene expression, and the deregulation of lncRNAs have been reported to be correlated with carcinogenesis and cancer progression. To explore the function of lncRNA in endometrial carcinoma, we analyzed the expression profiles of lncRNAs and coding genes in 3 paired endometrial carcinoma and adjacent non-tumor tissues, using a microarray. The results of microarray analysis indicated a significant difference in lncRNA and coding gene expression between endometrial carcinoma and their paired adjacent nontumor tissues. A total of 53 lncRNAs (fold change >2.0, p-value <0.05) were found to be differently expressed in endometrial carcinoma compared to the normal controls. Among these ASLNC04080 was the most significantly upregulated lncRNA in microarray data, highly expressed in 22 out of 24 endometrial carcinoma tissues and HEC-1-B cell line. ASLNC04080 is 1867nt in length, consist of 6 exons, and locates at 1 p35.3(chr1: -28905061 --28909492). In addition, 46 coding gene transcripts were differentially expressed (fold change >2.0, p-value <0.05) between endometrial carcinoma and adjacent non-tumor tissues. Pathway and gene ontology analysis demonstrated that these deregulated transcripts were involved in multiple signal pathways, biological processes, cellular components and molecular functions. Moreover, the ASLNC04080 lncRNA expression was correlated with 19 coding genes, and may contribute to endometrial carcinoma genesis and progression by co-regulating with coding gene. Expression inhibition of lncRNA ASLNC04080 in HEC-1-B cells caused repression of cell proliferation, increased cell apoptosis, and G1 phase arrest. These results suggested a potential function of ASLNC04080 in endometrial carcinoma genesis and progression. IntroductionEndometrial carcinoma, comprising of several types of malignancies arise from the endometrium or lining of the uterus, is the most common gynecologic malignancy among women in the United States, with an estimated 52,630 new case and 8,590 deaths in 2014 (1). Endometrial carcinoma cases in Chinese women increased in the last decade. Based on clinical features and pathogenesis endometrial carcinomas have been classified into two types (2). Type I endometrial carcinomas occur commonly in perimenopausal women, with low grade, related to obesity and estrogen exposure. Type II endometrial carcinomas are more common in older women, unrelated to hormone excess, with a worse outcome than Type I. Previous studies have reported that various gene mutations, and expression deregulation are related to endometrial carcinoma genesis. PTEN and K-ras mutations occur in Type I endometrial carcinomas, often with Wnt, AKT and PI3KCA deregulation. TP53 and PPP2R1A mutations are frequent in Type II endometrial carcinomas, with HER2/neu overexpression and p16 inactivation (3,4).LncRNAs are a spectrum of RNAs transcripted by RNA polymerase II, but not translated into proteins, with more than 200 nucleotides in length. LncRNAs whi...

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Section: Discussionmentioning

confidence: 77%

Microarray expression profile of lncRNAs and the upregulated ASLNC04080 lncRNA in human endometrial carcinoma

Zhai

et al. 2015

International Journal of Oncology

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“…More recently, VGSCs have also been found to be functionally expressed in "non-excitable" cells including astrocytes, macrophages, and human carcinoma cells. The latter include breast, prostate, colon, cervical, ovarian, non-small cell lung cancer, small cell lung cancer, lymphoma, mesothelioma, neuroblastoma, and melanoma (9,10). Importantly, where specifically tested, the predominant VGSC isoforms found were "neonatal" splice variants: Nav1.5 (breast and colon cancer) and Nav1.7 (prostate cancer) (9).…”

Section: Pathophysiological Mechanisms Of Cancer: Role Of Ion Channelsmentioning

confidence: 99%

Cancer in North Cyprus: 2. Biomedical Research Activities

Djamgoz¹,

Akün²,

Arslan³

et al. 2017

Cyprus J Med Sci

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This is the second part of a two-part review on the cancer status of North Cyprus. Here, we give an overview of the various areas of research on cancer. There are four main areas of ongoing biomedical investigation. First, monitoring of possible carcinogenic chemical factors in the environment (soil and water) is focused on arsenic. Arsenic has been found to exist broadly over the island at levels above the minimum level advised by the Environmental Protection Agency. At present, the source(s) of environmental arsenic is unclear. Second, pathophysiological mechanisms of breast and prostate cancer are being investigated with a focus on ion channels (particularly, the voltage-gated sodium channel) driving the metastatic process. In particular, the triangular relationship involving the channel, its persistent current component, and tumor hypoxia is being elucidated. Third, a further emerging theme is the association of microbiota with cancer. The possible roles of bacterial infections or microbiota in preventing, treating, or causing several types of cancers are among these projects. Fourth, a range of developments in nanotechnology is being considered. It is concluded that the island offers significant opportunities for internationally competitive, multi-faceted research on cancer.

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“…The significant involvement of the sigma-1 receptor in VGSC expression/activity may have wide-reaching clinical implications since VGSCs, and in particular nNa v 1.5, have already been proposed as a novel anti-metastatic target in a range of cancers (Fraser et al 2014;Onkal and Djamgoz 2009;Djamgoz and Onkal 2013).…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Sigma-1 receptors modulate neonatal Nav1.5 ion channels in breast cancer cell lines

et al. 2016

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co-immunoprecipitation experiments. Furthermore, application of sigma-1 drugs to the cells reduced the surface expression of nNa v 1.5 protein, which could explain how sigma-1 receptor activation could alter the metastatic behaviour of breast cancer cells. Overall, these results are consistent with the idea of a sigma-1 protein behaving like either a "chaperone" or a regulatory subunit associated with nNa v 1.5.

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Regulation of voltage-gated sodium channel expression in cancer: hormones, growth factors and auto-regulation

Cited by 124 publications

References 115 publications

Microarray expression profile of lncRNAs and the upregulated ASLNC04080 lncRNA in human endometrial carcinoma

Microarray expression profile of lncRNAs and the upregulated ASLNC04080 lncRNA in human endometrial carcinoma

Cancer in North Cyprus: 2. Biomedical Research Activities

Sigma-1 receptors modulate neonatal Nav1.5 ion channels in breast cancer cell lines

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