Regulation of transcription factors by neuronal activity

West, Anne E.; Griffith, Eric C.; Greenberg, Michael E.

doi:10.1038/nrn987

Cited by 531 publications

(438 citation statements)

References 124 publications

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“…On the other hand, CREB (cAMP response element binding protein) is a transcription factor and CREB-dependent gene expression is critical for a variety of cellular functions in the central nervous system. 23 As shown in Figure 6c, GFP-CREB fusion protein localized specifically to the nucleus, as expected, demonstrating that it was correctly targeted following expression. In addition, Figure 6a illustrates that seven out of seven neurons in this field were successfully transfected with GFP-Rab3A, suggesting that our improved protocol is applicable for expressing a variety of exogenous genes with high transfection efficiency.…”

Section: High Transfection Efficiency Achieved With Different Constructssupporting

confidence: 75%

High Ca2+-phosphate transfection efficiency enables single neuron gene analysis

2004

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Section: High Transfection Efficiency Achieved With Different Constructssupporting

confidence: 75%

High Ca2+-phosphate transfection efficiency enables single neuron gene analysis

2004

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“…It is indeed known that CREB/CRE participates in the modulation of plasticity and development of the mouse visual cortex and is also developmentally regulated in the LGN (Huang et al, 1999;Pham et al, 1999;Mower et al, 2002). CREB is activated by neuronal activity (West et al, 2002), and environmental enrichment increases immunoreactivity to CREB in the hippocampus (Williams et al, 2001). Therefore, it is conceivable that alterations in the expression-activation of CREB may account for the functional changes between EC and SC animals.…”

Section: Discussionmentioning

confidence: 99%

“…This drug is a specific inhibitor of the high-affinity phosphodiesterase type IV and thus activates the cAMP system via inhibition of cAMP breakdown, resulting in an increased phosphorylation of the transcription factor CREB (Tohda et al, 1996;Kato et al, 1998;Nakagawa et al, 2002). CREB is phosphorylated by cAMP-dependent kinase and regulates gene transcription by binding to the CRE on target genes (West et al, 2002). To study whether the cAMP/CREB system is involved in the accelerated maturation of visual acuity in EC mice, we treated SC mice with rolipram.…”

Section: Acceleration Of the Developmentally Regulated Cre-mediated Gmentioning

confidence: 99%

Acceleration of Visual System Development by Environmental Enrichment

Cancedda¹,

Putignano²,

Sale³

et al. 2004

J. Neurosci.

238

217

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Thus far, the developmental plasticity of the visual system has been studied by altering or reducing visual experience. Here, we investigated whether a complex sensory-motor stimulation, provided by rearing animals in an enriched environment, affects visual system development. We found that raising mice in this condition causes an earlier eye opening, a precocious development of visual acuity, and an accelerated decline of white matter-induced long-term potentiation. These effects are accompanied by a precocious cAMP response element-mediated gene expression and a significant increase of BDNF protein and GAD65/67 expression in enriched pups. In addition, we showed that enriched pups experienced higher levels of licking behavior provided by adult females. Thus, rearing mice from birth in an enriched environment leads to a conspicuous acceleration of visual system development as ascertained at behavioral, electrophysiological, and molecular level.

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“…The mechanisms by which stress initiates the modifications in the composition of splicing-related proteins and the sustained elevation in SC35 is still unknown; however, neuronal activity modulations are likely involved. 47 Acetylcholine levels are notably elevated under stress, 13 and acetylcholine modulates the activity of neurons responding to other neurotransmitters as well (eg glutamate, GABA 48 ). Therefore, the capacity to downregulate acetylcholine levels throughout the brain by the soluble AChE-R may be important.…”

Section: Sc35 In Long-term Stress Responsesmentioning

confidence: 99%

SC35 promotes sustainable stress-induced alternative splicing of neuronal acetylcholinesterase mRNA

et al. 2005

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Long-lasting alternative splicing of neuronal acetylcholinesterase (AChE) pre-mRNA occurs during neuronal development and following stress, altering synaptic properties. To explore the corresponding molecular events, we sought to identify mRNAs encoding for abundant splicing factors in the prefrontal cortex (PFC) following stress. Here we show elevated levels of the splicing factor SC35 in stressed as compared with naïve mice. In cotransfections of COS-1 and HEK293 cells with an AChE minigene allowing 3 0 splice variations, SC35 facilitated a shift from the primary AChE-S to the stress-induced AChE-R variant, while ASF/SF2 caused the opposite effect. Transfection with chimeric constructs comprising of SC35 and ASF/SF2 RRM/RS domains identified the SC35 RRM as responsible for AChE mRNA's alternative splicing. In poststress PFC neurons, increased SC35 mRNA and protein levels coincided with selective increase in AChE-R mRNA. In the developing mouse embryo, cortical progenitor cells in the ventricular zone displayed transient SC35 elevation concomitant with dominance of AChE-R over AChE-S mRNA. Finally, transgenic mice overexpressing human AChE-R, but not those overexpressing AChE-S, showed significant elevation in neuronal SC35 levels, suggesting a reciprocal reinforcement process. Together, these findings point to an interactive relationship of SC35 with cholinergic signals in the long-lasting consequences of stress on nervous system plasticity and development. Molecular Psychiatry (2005) 10, 985-997.

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Regulation of transcription factors by neuronal activity

Cited by 531 publications

References 124 publications

High Ca2+-phosphate transfection efficiency enables single neuron gene analysis

High Ca2+-phosphate transfection efficiency enables single neuron gene analysis

Acceleration of Visual System Development by Environmental Enrichment

SC35 promotes sustainable stress-induced alternative splicing of neuronal acetylcholinesterase mRNA

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