Regulation of Transcription by DNA‐Bound Non‐histone Nuclear Proteins

Crépin, Michel; Dastugue, B.

doi:10.1111/j.1432-1033.1979.tb13281.x

Cited by 16 publications

(9 citation statements)

References 28 publications

(31 reference statements)

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“…Only one major hybridizing band at 2.1 kb (L-type mRNA) was seen with probe 22~1 under our experimental conditions. Since this probe is capable of hybridizing also with the 1.9 kb M-type mRNA [2], we conclude that the thyroid status did not shift the pattern of expression from the L-type towards the M-type mRNA. This was confirmed by dot blot analysis with the cDNA probe 5c2-Ddel which is specific for the M-type mRNA.…”

Section: Effect Of the Thyroid Status On Liver Pfk-2 Mrnamentioning

confidence: 73%

“…immunotitra~on was carried out as described [3] using an antibody (BCL-2) raised against purified chicken liver PFK-2. Abbreviations: PFK-2, 6-phosphofructo-2-kinase; Tr, triiodothyronine; T.1, thyroxine cDNA probes [2,7] specific for the L-type (22c2-Sau3A) and the Mtype (ScS-DdeI) PFK-2 mRNA, and one common to both isozymes (22cl), were multiprime-labelled with [cu-'2P]dCTP to a specific activity of 2-4 x 10' cpm/pg. A chicken@-actin probe was also used as an internal control.…”

Section: Methodsmentioning

confidence: 99%

“…Dot blot 1131 RNA-cDNA hybridi~tion was performed on RNA denatured with formaldehyde/formamide, using prehybridization and hybridization conditions as described [3]. Filters were washed for 30 min at room temperature, then for two periods of 30 min at 50°C in 2 x SSC (SSC [2], total RNA was resuspended in a denaturing loading buffer, heat denatured, and electrophoresed [14] in the presence of 1 pglmi ethidium bromide. The gel was photographed under UV light to record migration of RNA molecular weight markers (BRL) and to monitor the quantity of RNA loaded by densitometry of 28 S and 18 S rRNA.…”

Section: Methodsmentioning

confidence: 99%

“…RNA was transferred to a nylon membrane (Hybond N, Amersham) in 10 x SSC for at least 12 h, then fixed onto the membrane by UV irradiation for 5 min. Filters were prehybridized and hybridized as described [2]…”

Section: Methodsmentioning

confidence: 99%

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Thyroid hormone stimulates expression of 6‐phosphofructo‐2‐kinase in rat liver

Wall¹,

Hove²,

Crepin³

et al. 1989

FEBS Letters

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The activity of liver 6-phosphofructo-2-kinase (PFK-2), the enzyme that catalyses the synthesis of fructose 2,dbisphosphate, was markedly decreased in hypothyroid rats and partially restored after 3 days of treatment with triiodothyronine. The changes in PFK-2 activity were accompanied by parallel changes in enzyme content measured by immunotitration and in PFK-2 mRNA determined by dot blot and Northern blot hybridization with cDNA probes. It is concluded that thyroid hormone stimulates liver PFK-2 gene expression by a pre-translational mechanism.

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Section: Effect Of the Thyroid Status On Liver Pfk-2 Mrnamentioning

confidence: 73%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Thyroid hormone stimulates expression of 6‐phosphofructo‐2‐kinase in rat liver

Wall¹,

Hove²,

Crepin³

et al. 1989

FEBS Letters

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“…DNA and protein . chromatin complexes.The experimental conditions used for this study have made it possible to analyse the relationships between the binding properties of non-histone proteins and the effect on the DNA and chromatin transcription process (see the following paper [21]). …”

mentioning

confidence: 99%

Interaction of Non‐histone Proteins with DNA and Chromatin from Drosophila and Mouse Cells

Dastugue¹,

Crépin²

1979

European Journal of Biochemistry

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The specificity of the binding of purified non-histone proteins to DNA has been investigated through two types of experiments. Using a nitrocellulose filter assay at a low protein/DNA ratio, the binding of mouse non-histone proteins to mouse DNA was twice as great as the binding of mouse non histone protein to Drosophilu DNA. The reverse experiment using Drosophila nonhistone protein confirmed the interpretation that some protein . DNA complexes were specific. Protein . DNA complexes isolated by gel filtration chromatography indicated that 20% or 10% of the non-histone protein was bound to homologous or heterologous DNA respectively. Purified non-histone proteins bound with lower efficiency (1 5 %) than unpurified but with higher specificity to soluble chromatin than to naked DNA. This binding did not result from an exchange between chromatin non-histone proteins and purified non-histone proteins added in excess. DNA-bound and chromatin-bound proteins were analysed on polyacrylamide gels. Whereas no major qualitative differences were observed with DNA-bound proteins, some proteins bound to homologous mouse chromatin were different from those bound to heterologous Drosophila chromatin. These results suggest a possible role of DNA-bound non-histone proteins in the regulation of gene expression.In bacteria and viruses, the expression of genetic material has been shown to be controlled, at least partly, by DNA-binding proteins. Such regulatory mechanisms could exist in higher organisms. Nuclear non-histone proteins exhibit heterogeneity, specificity [ l -41, rapid turnover [5,6], modifications, e.g. phosphorylation [7,8] and hormone-binding [9,10], all of which are expected in molecules involved in gene regulation. Furthermore, several investigators have described the binding of non-histone protein to DNA [l 1 -191. The specificity of the protein-DNA interaction has been approached experimentally by the use of DNA-affinity chromatography [ l l , 14,16,17], sucrose gradient centrifugation [12,13,19] and filtration assay [15, IS]. By these different methods, it has been shown that there was a specificity in the formation of DNA . protein complexes.The addition of non-histone proteins to chromatin has been proposed to modify transcription in a manner characteristic of the tissue from which they were derived [20]. Several mechanisms could account forDNAase I or deoxyribonucleate 5'-oligonucleotidohydrolase (EC 3.1.4.5). this action. No direct correlation has been shown between the formation of DNA . protein complexes and the described effect of non-histone protein on chromatin transcription, since bound non-histone proteins have not been analysed for this effect.In this paper, we describe the binding of a class of non-histone proteins to native DNA and to chromatin. Dealing with a low protein/DNA ratio, we demonstrate a limited number of specific DNA binding sites in both cases. We also define conditions that should make it possible to isolate quantitatively the protein . DNA and protein . chromatin complexes.The experi...

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Distribution of non‐histone proteins between micrococcal nuclease sensitive and nuclease resistant chromatin from chicken cells with active and inactive genomes

Kiliańska

Klyszejko-Stefanowicz

1984

Cell Biochemistry & Function

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Chicken liver and erythrocyte nuclei were separated by mild treatment with micrococcal nuclease into nuclease sensitive (NS) and nuclease resistant (NR) fractions, differing in chemical composition and transcriptional activity in vitro. Nuclei, NS and NR fractions of both tissues were fractionated by hydroxyapatite chromatography into three groups of non-histone chromatin proteins (NHCP) and characterized by SDS-polyacrylamide gel electrophoresis. Some differences in the molecular distribution of non-histone proteins of chicken liver and erythrocytes between nuclease sensitive and resistant parts of chromatin have been described.

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Regulation of Transcription by DNA‐Bound Non‐histone Nuclear Proteins

Cited by 16 publications

References 28 publications

Thyroid hormone stimulates expression of 6‐phosphofructo‐2‐kinase in rat liver

Thyroid hormone stimulates expression of 6‐phosphofructo‐2‐kinase in rat liver

Interaction of Non‐histone Proteins with DNA and Chromatin from Drosophila and Mouse Cells

Distribution of non‐histone proteins between micrococcal nuclease sensitive and nuclease resistant chromatin from chicken cells with active and inactive genomes

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