Regulation of topoisomerase II α and β in HIV-1 infected and uninfected neuroblastoma and astrocytoma cells: Involvement of distinct nordihydroguaretic acid sensitive inflammatory pathways

Mandraju, Raj Kumar; Kondapi, Anand K.

doi:10.1016/j.abb.2007.01.026

Cited by 11 publications

(4 citation statements)

References 35 publications

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“…Topo IIα is closely related to the occurrence, development, diagnosis, prognosis and treatment of cancer. Otherwise, Topo IIβ is associated with non-proliferating function and believed to maintain the integrity of nuclear chromatin [4,5]. …”

Section: Introductionmentioning

confidence: 99%

Genistein Inhibition of Topoisomerase IIα Expression Participated by Sp1 and Sp3 in HeLa Cell

Zhou

Yunli

et al. 2009

IJMS

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Genistein (4′, 5, 7-trihydroxyisoflavone) is an isoflavone compound obtained from plants that has potential applications in cancer therapy. However, the molecular mechanism of the action of genistein on cancer cell apoptosis is not well known. In this study, we investigated the effect of genistein on topoisomerase II-α (Topo IIα), an important protein involved in the processes of DNA replication and cell proliferation. The results revealed that inhibition of Topo IIα expression through the regulation of Specificity protein 1 and Specificity protein 3 may be one of the reasons for genistein’s induction of HeLa cell apoptosis.

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Section: Introductionmentioning

confidence: 99%

Genistein Inhibition of Topoisomerase IIα Expression Participated by Sp1 and Sp3 in HeLa Cell

Zhou

Yunli

et al. 2009

IJMS

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“…8D) levels in infected cells. These results suggest that nano-curcumin effectively blocks HIV-1-mediated inflammatory responses [21] and thus affects viral cDNA synthesis.…”

Section: Resultsmentioning

confidence: 80%

Curcumin-Loaded Apotransferrin Nanoparticles Provide Efficient Cellular Uptake and Effectively Inhibit HIV-1 Replication In Vitro

et al. 2011

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BackgroundCurcumin (diferuloylmethane) shows significant activity across a wide spectrum of conditions, but its usefulness is rather limited because of its low bioavailability. Use of nanoparticle formulations to enhance curcumin bioavailability is an emerging area of research.Methodology/Principal FindingsIn the present study, curcumin-loaded apotransferrin nanoparticles (nano-curcumin) prepared by sol-oil chemistry and were characterized by electron and atomic force microscopy. Confocal studies and fluorimetric analysis revealed that these particles enter T cells through transferrin-mediated endocytosis. Nano-curcumin releases significant quantities of drug gradually over a fairly long period, ∼50% of curcumin still remaining at 6 h of time. In contrast, intracellular soluble curcumin (sol-curcumin) reaches a maximum at 2 h followed by its complete elimination by 4 h. While sol-curcumin (GI50 = 15.6 µM) is twice more toxic than nano-curcumin (GI50 = 32.5 µM), nano-curcumin (IC50<1.75 µM) shows a higher anti-HIV activity compared to sol-curcumin (IC50 = 5.1 µM). Studies in vitro showed that nano-curcumin prominently inhibited the HIV-1 induced expression of Topo II α, IL-1β and COX-2, an effect not seen with sol-curcumin. Nano-curcumin did not affect the expression of Topoisomerase II β and TNF α. This point out that nano-curcumin affects the HIV-1 induced inflammatory responses through pathways downstream or independent of TNF α. Furthermore, nano-curcumin completely blocks the synthesis of viral cDNA in the gag region suggesting that the nano-curcumin mediated inhibition of HIV-1 replication is targeted to viral cDNA synthesis.ConclusionCurcumin-loaded apotransferrin nanoparticles are highly efficacious inhibitors of HIV-1 replication in vitro and promise a high potential for clinical usefulness.

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“…Consistently, Zhou et al have illustrated that endothelial progenitor cell-derived exosomes could reduce inflammation in mice with LPS-induced ALI [ 24 ]. A recent study has verified that overexpressed miR-125b-5p has the capacity to restrain inflammatory state in macrophages [ 25 ], and it has been figured out that the expression of TOP2A is concomitant with inflammation in human immunodeficiency virus (HIV)-1 infection [ 26 ]. Additionally, we have pointed out that the exosomes and amplified miR-125b-5p promoted VEGF in lung tissues while declined serum VEGF level in ALI mice.…”

Section: Discussionmentioning

confidence: 99%

Upregulation of endothelial cell-derived exosomal microRNA-125b-5p protects from sepsis-induced acute lung injury by inhibiting topoisomerase II alpha

Jiang

Shen

et al. 2021

Inflamm. Res.

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Regulation of topoisomerase II α and β in HIV-1 infected and uninfected neuroblastoma and astrocytoma cells: Involvement of distinct nordihydroguaretic acid sensitive inflammatory pathways

Cited by 11 publications

References 35 publications

Genistein Inhibition of Topoisomerase IIα Expression Participated by Sp1 and Sp3 in HeLa Cell

Genistein Inhibition of Topoisomerase IIα Expression Participated by Sp1 and Sp3 in HeLa Cell

Curcumin-Loaded Apotransferrin Nanoparticles Provide Efficient Cellular Uptake and Effectively Inhibit HIV-1 Replication In Vitro

Upregulation of endothelial cell-derived exosomal microRNA-125b-5p protects from sepsis-induced acute lung injury by inhibiting topoisomerase II alpha

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