Regulation of TonEBP transcriptional activator in MDCK  cells following changes in ambient tonicity

Neuhofer, Wolfgang; Woo, Seung Kyoon; Na, Ki Young; Grünbein, Rita; Park, Won Kun; Nahm, Ohnn; Beck, Franz‐Xaver; Kwon, H. Moo

doi:10.1152/ajpcell.00216.2002

Cited by 43 publications

(53 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, inhibition of Na-K-ATPase by ouabain leads to decreased activity of TonEBP/OREBP despite a marked increase in the intracellular ionic strength. It was suggested that, because isotonic swelling is known to occur under these conditions, dilution of the cytoplasmic constituents inhibits the activity of TonEBP/OREBP (36). However, the present study affords an alternative explanation as the effect of ouabain on TonEBP/ OREBP is associated with suppression of ROS formation (Fig.…”

Section: Discussionmentioning

confidence: 61%

“…Hypertonicity shrinks cells by osmosis, resulting in increased concentration of all intracellular components, including intracellular ions (36) and macromolecules (6). Decrease in cell volume (12), increase in intracellular ionic strength (36), and increased macromolecular crowding (6) are all candidates to be sensors that trigger cellular responses to hypertonicity.…”

Section: Discussionmentioning

confidence: 99%

“…Decrease in cell volume (12), increase in intracellular ionic strength (36), and increased macromolecular crowding (6) are all candidates to be sensors that trigger cellular responses to hypertonicity. Their relative importance remains controversial.…”

Section: Discussionmentioning

confidence: 99%

“…Results are expressed relative to control at 300 mosmol/kgH2O. regardless of the external tonicity (36). On the other hand, inhibition of Na-K-ATPase by ouabain leads to decreased activity of TonEBP/OREBP despite a marked increase in the intracellular ionic strength.…”

Section: Discussionmentioning

confidence: 99%

“…In Madin-Darby canine kidney cells, ouabain reduces high NaCl-dependent increase of AR, SMIT and BGT1 mRNA abundance and of TonEBP/OREBP protein abundance (36). Those effects were attributed to cell swelling caused by ouabain and were proposed as evidence that TonEBP/OREBP activity is regulated by cell volume, as well as by intracellular ionic strength (36). However, ouabain also reduces the high NaCl-induced increase in superoxide in rat medullary thick ascending limbs (33).…”

Section: Increase Of Ros In the Absence Of High Nacl Is Not Sufficienmentioning

confidence: 99%

See 4 more Smart Citations

Increased reactive oxygen species contribute to high NaCl-induced activation of the osmoregulatory transcription factor TonEBP/OREBP

Zhou¹,

Ferraris²,

Cai³

et al. 2005

American Journal of Physiology-Renal Physiology

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Increase Of Ros In the Absence Of High Nacl Is Not Sufficienmentioning

confidence: 99%

See 3 more Smart Citations

Increased reactive oxygen species contribute to high NaCl-induced activation of the osmoregulatory transcription factor TonEBP/OREBP

Zhou¹,

Ferraris²,

Cai³

et al. 2005

American Journal of Physiology-Renal Physiology

View full text Add to dashboard Cite

show abstract

Hypertonic upregulation of betaine transport in renal cells is blocked by a proteasome inhibitor

Lammers

Beck

Chu

et al. 2005

Cell Biochemistry & Function

View full text Add to dashboard Cite

The renal betaine transporter (BGT1) protects cells in the hypertonic medulla by mediating uptake and accumulation of the osmolyte betaine. Transcription plays an essential role in upregulating BGT1 transport in MDCK cells subjected to hypertonic stress. During hypertonic stress, the abundance of the transcription factor TonEBP increases and it shifts from the cytoplasm to the nucleus where it activates transcription of the BGT1 gene. Little is known about post-transcriptional regulation of BGT1 protein. In the presence of the proteasome inhibitor MG-132, which blocked nuclear translocation of TonEBP, the hypertonic upregulation of BGT1 protein and transport was prevented and cell viability in hypertonic medium was impaired over 24 h. Urea also prevented the hypertonic upregulation of BGT1 protein and transport, but did not interfere with TonEBP translocation and cell viability. Shorter treatments of hypertonic cells with MG-132 avoided viability problems and produced dose-dependent inhibition of translocation and transport. When stably transfected MDCK cells that over-expressed BGT1 were treated for 6 h with hypertonic medium containing 3 microM MG-132, there was 43% inhibition of nuclear translocation, 83% inhibition of BGT1 transport, and no change in viability. While other proteasome functions may be involved, these data are consistent with a critical role for nuclear translocation of TonEBP in upregulation and membrane insertion of BGT1 protein.

show abstract

Auswirkungen von Natriumchlorid auf das Genexpressionsprofil parodontaler Ligamentfibroblasten bei Dehnung

et al. 2020

View full text Add to dashboard Cite

Purpose During orthodontic tooth movement, pressure and tension zones develop in the periodontal ligament, and periodontal ligament fibroblasts (PDLF) become exposed to mechanical strain. Enhanced salt (NaCl) concentrations are known to modulate responses of PDLF and immune cells to different stimuli like mechanical strain. Here, we investigated the impact of tensile strain on the gene expression profile of PDLF under normal (NS) and high salt (HS) conditions. Methods After preincubation under NS or HS (+40 mM NaCl in medium) conditions for 24 h, PDLF were stretched 16% for 48 h using custom-made spherical cap silicone stamps using an established and published setup. After determination of cell number and cytotoxicity, we analyzed expression of genes involved in extracellular matrix reorganization, angiogenesis, bone remodeling, and inflammation by quantitative real-time polymerase chain reaction (RT-qPCR). Results Tensile strain did not affect the expression of genes involved in angiogenesis or extracellular matrix reorganization by PDLF, which however modulate inflammatory responses and bone remodeling in reaction to 16% static tensile strain. Salt (NaCl) treatment triggered enhanced extracellular matrix formation, expression of cyclooxygenase 2 and bone metabolism in PDLF during tensile strain. Conclusions Salt (NaCl) consumption may influence orthodontic tooth movement and periodontal bone loss via modulation of extracellular matrix and bone metabolism. Excessive salt intake during orthodontic therapy may cause adverse effects regarding periodontal inflammation and bone resorption.

show abstract

Regulation of TonEBP transcriptional activator in MDCK cells following changes in ambient tonicity

Cited by 43 publications

References 28 publications

Increased reactive oxygen species contribute to high NaCl-induced activation of the osmoregulatory transcription factor TonEBP/OREBP

Increased reactive oxygen species contribute to high NaCl-induced activation of the osmoregulatory transcription factor TonEBP/OREBP

Hypertonic upregulation of betaine transport in renal cells is blocked by a proteasome inhibitor

Auswirkungen von Natriumchlorid auf das Genexpressionsprofil parodontaler Ligamentfibroblasten bei Dehnung

Contact Info

Product

Resources

About