Regulation of three beta-tubulin mRNAs during rat brain development.

Ginzburg, Irith; Teichman, Adriana von; Dodemont, H.; Behar, Leah; Littauer, Uriel Z.

doi:10.1002/j.1460-2075.1985.tb04133.x

Cited by 53 publications

(20 citation statements)

References 43 publications

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“…Additionally, fetal brain extract contained a greater level of the class II isotype than the TUB2.1-reacting ␤-tubulin, which is in contrast to the levels detected in adult brain extract. A previous study showed that the mRNA level of class II and III isotypes in the cerebral cortex decreased during postnatal development [Ginzburg et al, 1985;Jiang and Oblinger, 1992]. Taken together, these results suggest that the class II isotype is a major component of the total ␤-tubulin in certain fetal tissues and is developmentally down-regulated.…”

Section: Discussionsupporting

confidence: 57%

Distribution of the class II β‐tubulin in developmental and adult rat tissues

Arai¹,

Shibutani²,

Matsuda³

2002

Cell Motil. Cytoskeleton

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During a screen of monoclonal antibodies raised against a cytoskeletal preparation of neonatal rat cerebrum, we have identified a monoclonal antibody, MAb58A, that is specific for the class II beta-tubulin isotype. Immunoscreening of a rat brain cDNA library using MAb58A yielded the cDNA retaining a class II-specific nucleotide sequence. The specificity of MAb58A to the class II beta-tubulin isotype was confirmed by immunoreactivity to synthetic peptides corresponding to isotype-specific sequence of class I, II, III, IVa, or IVb. Further, the results of an immunoassay against a series of overlapping octapeptides derived from a class II-specific region revealed that the antibody epitope was a heptapeptide that consists of Glu-Glu-Glu-Glu-Gly-Glu-Asp (EEEEGED). Immunoblot analysis revealed that the class II isotype represented a significant portion of beta-tubulin present in the adrenal gland, brain, and testis of adult rats. In fetal tissues, this isotype was detected in skeletal muscle, as well as in the brain. Immunohistochemically, MAb58A reacted predominantly with components of the developing rat nervous system, such as migrating neuroblasts, peripheral nerves and ganglion cells, and sensory organs. MAb58A-immunoreactivity was also found in developing skeletal and smooth muscle cells, chondrocytes, and vascular endothelia. In adults, MAb58A-immunoreactivity was remarkably diminished, but persisted in peripheral nerves and ganglion cells, chondrocytes, and capillary components. Together, our results demonstrate that MAb58A is specific for the class II beta-tubulin isotype, which may retain an embryonic nature in both neuronal and non-neuronal tissues.

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Section: Discussionsupporting

confidence: 57%

Distribution of the class II β‐tubulin in developmental and adult rat tissues

Arai¹,

Shibutani²,

Matsuda³

2002

Cell Motil. Cytoskeleton

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“…This inverse relationship is realized in axons throughout the vertebrates including the large caliber (500 m) CNS Mauthner axons of fish (Faundez and Alvarez, 1986). Although there are multiple changes in the synthesis of cytoskeletal elements in axotomized neurons, the same inverse relationship between MT density and NF content is observed as fibers regenerate and mature (Ginzburg et al, 1985;Hoffman and Cleveland, 1988;Tashiro and Komiya, 1992).…”

Section: Discussionmentioning

confidence: 94%

“…Neuronal development and axonal regeneration are associated with complex programs of temporally and spatially regulated tubulin isotype expression and such changes are reflected in the density of the axonal MT network (Ginzburg et al, 1985;Faundez and Alvarez, 1986;Hoffman and Cleveland, 1988;Tashiro and Komiya, 1992). Previous investigation of NFHLacZ transgenic mice showed that the amount of accumulated tubulin and MAPs was not dramatically changed (Tu et al, 1997;Riederer et al, 2003).…”

Section: Accumulation Of Tubulin Transcripts and Proteinsmentioning

confidence: 99%

Neurofilaments Bind Tubulin and Modulate Its Polymerization

Bocquet¹,

Bergès²,

Frank³

et al. 2009

J. Neurosci.

104

118

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“…The HSC 70 mRNA (T. K. C. Leung, C. Hall, C. Monfries & L. Lim, unpublished work) present in both free and membrane-bound polyribosomes, shown for comparison (Fig. 5a), undergoes a developmental decrease in rat forebrain, as does tubulin mRNA (Gozes et al, 1977;Ginzburg et al, 1985;Fig. 6c).…”

Section: Tissue Distribution Of M444 and M1622 Mrnasmentioning

confidence: 96%

Expression and developmental regulation of two unique mRNAs specific to brain membrane-bound polyribosomes

Hall¹,

Lowndes²,

Leung³

et al. 1987

Biochemical Journal

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Translation in vitro of membrane-bound polyribosomal mRNAs from rat brain has shown several to be developmentally regulated [Hall & Lim (1981) Biochem. J. 196,[327][328][329][330][331][332][333][334][335][336]. Here we describe the isolation and characterization of cDNAs corresponding to two such brain mRNAs. One cDNA (M444) hybridselected a 0.95 kb mRNA directing the synthesis in vitro of a 21 kDa pl-6.3 polypeptide, which was processed in vitro by microsomal membranes. A second cDNA (M1622) hybridized to a 2.2 kb mRNA directing the synthesis of a 55 kDa pl-5.8 polypeptide. Both mRNAs were specific to membrane-bound polyribosomes. Restriction maps of the corresponding genomic DNA sequences are consistent with both being single copy. The two mRNAs were present in astrocytic and neuronal cultures, but not in liver or spleen or in neuroblastoma or glioma cells. The two mRNAs were differently regulated during brain development. In the developing forebrain there was a gradual and sustained increase in M444 mRNA during the first 3 weeks post partum, whereas M 1622 mRNA appeared earlier and showed no further increase after day 10. In the cerebellum the developmental increase in M444 mRNA was biphasic. After a small initial increase there was a decrease in this mRNA at day 10, coincident with high amounts of M1622 mRNA. This was followed by a second, larger, increase in M444 mRNA, when amounts of M1622 mRNA were constant. The contrasting changes in these two mRNAs in the developing cerebellum are of particular interest, since they occur during an intensive period of cell proliferation, migration and altering neural connectivity. As these mRNAs are specific to differentiated neural tissue, they represent useful molecular markers for studying brain differentiation.

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Regulation of three beta-tubulin mRNAs during rat brain development.

Cited by 53 publications

References 43 publications

Distribution of the class II β‐tubulin in developmental and adult rat tissues

Distribution of the class II β‐tubulin in developmental and adult rat tissues

Neurofilaments Bind Tubulin and Modulate Its Polymerization

Expression and developmental regulation of two unique mRNAs specific to brain membrane-bound polyribosomes

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