Regulation of Thermostable Direct Hemolysin and Biofilm Formation of Vibrio parahaemolyticus by Quorum-Sensing Genes luxM and luxS

Guo, M.; Fang, Zhijia; Sun, Lijun; Sun, Dongfang; Wang, Yaling; Li, Can; Wang, Rundong; Liu, Yang; Hu, Hanqiao; Liu, Ying; Xu, Defeng; Gooneratne, Ravi

doi:10.1007/s00284-018-1508-y

Cited by 32 publications

(15 citation statements)

References 40 publications

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“…Another study has shown that the luxM/luxS-dependent quorum sensing mediates the regulation of the hemolytic activity along with biofilm formation in V. parahaemolyticus. luxM downregulates tdh gene expression, and therefore compromises the hemolytic activity, while luxS upregulates the expression of the hemolysin (Guo et al, 2018). Also, HN-S (nucleoid-associated DNA binding regulator) and Hfq (a global transcription regulator) have been shown to repress the production of TDH (Nakano et al, 2008;Sun et al, 2014).…”

Section: Secretion and Regulation Of Tdhmentioning

confidence: 99%

Current Perspective on the Membrane-Damaging Action of Thermostable Direct Hemolysin, an Atypical Bacterial Pore-forming Toxin

Verma

Chattopadhyay

2021

Front. Mol. Biosci.

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Thermostable direct hemolysin (TDH) is the major virulence determinant of the gastroenteric bacterial pathogen Vibrio parahaemolyticus. TDH is a membrane-damaging pore-forming toxin (PFT). TDH shares remarkable structural similarity with the actinoporin family of eukaryotic PFTs produced by the sea anemones. Unlike most of the PFTs, it exists as tetramer in solution, and such assembly state is crucial for its functionality. Although the structure of the tetrameric assembly of TDH in solution is known, membrane pore structure is not available yet. Also, the specific membrane-interaction mechanisms of TDH, and the exact role of any receptor(s) in such process, still remain unclear. In this mini review, we discuss some of the unique structural and physicochemical properties of TDH, and their implications for the membrane-damaging action of the toxin. We also present our current understanding regarding the membrane pore-formation mechanism of this atypical bacterial PFT.

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Section: Secretion and Regulation Of Tdhmentioning

confidence: 99%

Current Perspective on the Membrane-Damaging Action of Thermostable Direct Hemolysin, an Atypical Bacterial Pore-forming Toxin

Verma

Chattopadhyay

2021

Front. Mol. Biosci.

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“…The QS system consists of signals and receptors, quorum sensing regulators, and other genes under control. The fold difference of opaR upregulated was about 2.08, and luxS was downregulated by about 2.32 ( Guo et al., 2018 ) ( Table 2 ). opaR is considered a major QS regulator involved in colony morphology, virulence and other biophysical aspects ( Burke et al., 2015 ), and LuxS is a key enzyme in the LuxS/AI-2 QS system.…”

Section: Resultsmentioning

confidence: 99%

Comparative Transcriptome Analysis Reveals Regulatory Factors Involved in Vibrio Parahaemolyticus Biofilm Formation

Wang

et al. 2022

Front. Cell. Infect. Microbiol.

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Vibrio parahaemolyticus biofilm poses a serious threat to food safety and human health. However, there is limited knowledge of transcriptional regulatory mechanism during the biofilm formation of this organism. Hence, the RNA sequencing technique was employed to compare the differences in transcriptome profiles between planktonic and biofilm state of V. parahaemolyticus ATCC33847 in this study. Collections of mRNA from planktonic and biofilm cells cultured at 25°C for 36 h were sequenced by studying their biological characteristics. The results showed that there were significant differences in the expression levels of 956 genes in biofilms compared with planktonic cells. These differences suggested that two-component regulatory system (TCS) and quorum sensing (QS) regulated V. parahaemolyticus biofilm formation by affecting important factors such as flagellar motility, Extracellular polymeric substance (EPS) secretion, tripartite ATP-independent (TRAP) transport system and ATP binding cassette (ABC) transport system. The present work in transcriptomics serves as a basis for future studies examining the complex network systems that regulate bacterial biofilm formation.

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“…We observed that the promoter activity of P epsA - lux and P mfpABC - lux were all decreased significantly in Δ robA Δ opaR and Δ robA Δ cpsQ compared to that in the WT and Δ robA strains ( Figures 6E,F ). V. parahaemolyticus can produce three types of autoinducer, including harveyi autoinducer 1, autoinducer 2, and cholerae autoinducer 1, which are synthesized by LuxM, LuxS, and CqsA, respectively ( Guo et al, 2018 ; Wu K. et al, 2019 ). To explore which autoinducer participates in the regulation of RobA to OpaR and CpsQ, we then created a series of genes deletion mutants of the autoinducer synthases.…”

Section: Resultsmentioning

confidence: 99%

Identification of LuxR Family Regulators That Integrate Into Quorum Sensing Circuit in Vibrio parahaemolyticus

Zhong

Liu

et al. 2021

Front. Microbiol.

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Vibrio parahaemolyticus is one of the most important food-borne pathogens that cause economic and public health problems worldwide. Quorum sensing (QS) is a way for the cell-cell communication between bacteria that controls a wide spectrum of processes and phenotypic behaviors. In this study, we performed a systematic research of LuxR family regulators in V. parahaemolyticus and found that they influence the bacterial growth and biofilm formation. We then established a QS reporter plasmid based on bioluminescence luxCDABE operon of Vibrio harveyi and demonstrated that several LuxR family regulators integrated into QS circuit in V. parahaemolyticus. Thereinto, a novel LuxR family regulator, named RobA, was identified as a global regulator by RNA-sequencing analyses, which affected the transcription of 515 genes in V. parahaemolyticus. Subsequent studies confirmed that RobA regulated the expression of the exopolysaccharides (EPS) synthesis cluster and thus controlled the biofilm formation. In addition, bioluminescence reporter assays showed that RobA plays a key role in the QS circuit by regulating the expression of opaR, aphA, cpsQ-mfpABC, cpsS, and scrO. We further demonstrated that the regulation of RobA to EPS and MfpABC depended on OpaR and CpsQ, which combined the QS signal with bis-(3′-5′)-cyclic dimeric GMP to construct a complex regulatory network of biofilm formation. Our data provided new insights into the bacterial QS mechanisms and biofilm formation in V. parahaemolyticus.

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Regulation of Thermostable Direct Hemolysin and Biofilm Formation of Vibrio parahaemolyticus by Quorum-Sensing Genes luxM and luxS

Cited by 32 publications

References 40 publications

Current Perspective on the Membrane-Damaging Action of Thermostable Direct Hemolysin, an Atypical Bacterial Pore-forming Toxin

Current Perspective on the Membrane-Damaging Action of Thermostable Direct Hemolysin, an Atypical Bacterial Pore-forming Toxin

Comparative Transcriptome Analysis Reveals Regulatory Factors Involved in Vibrio Parahaemolyticus Biofilm Formation

Identification of LuxR Family Regulators That Integrate Into Quorum Sensing Circuit in Vibrio parahaemolyticus

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