Regulation of the utilization of glucose and aromatic substrates in four strains of Pseudomonas putida

Reber, H; Kaiser, Paul

doi:10.1007/bf00459527

Cited by 22 publications

(11 citation statements)

References 12 publications

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“…However, the rates of consumption of the 'poor' substrates are reduced to a much greater extent (4540%) than those of the 'good' substrates (2073, suggesting that there is a preference for the latter. Reber & Kaiser (1981) have also reported co-utilization of glucose and aromatic substrates, in Pseudomonas putida.…”

Section: Discussionmentioning

confidence: 90%

Catabolite Effects On Enzyme Induction And Substrate Utilization In Rhizobium leguminosarum

et al. 1983

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The catabolism of L-histidine and p-hydroxybenzoate in Rhizobium leguminosarum 3841 is inducible. Glucose or succinate have relatively little effect on the induction of histidase whereas they produce about 50% repression of the p-hydroxybenzoate catabolic system. When grown in the presence of a mixture of carbon sources, e.g. glucose plus histidine, or succinate plus phydroxybenzoate, the cells co-utilize both substrates. However, consumption of histidine and phydroxybenzoate (which support slower growth rates than glucose or succinate) is substantially reduced in comparison with that of glucose or succinate.

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Section: Discussionmentioning

confidence: 90%

Catabolite Effects On Enzyme Induction And Substrate Utilization In Rhizobium leguminosarum

et al. 1983

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“…Research is also being pursued regarding the cometabolism of toxic compounds which will not serve as sole source of carbon for the degrading population (9). Additionally, recent efforts have addressed simultaneous metabolism of multiple substrates (10,11), some of which are hazardous organics (e.g., méthylène chloride and p-nitrophenol; 6,12,13,14). Multiple substrate metabolism may provide the opportunity to effect biodégradation of toxic organic compounds at rates well above those possible in systems where toxic substrates are the sole carbon and energy sources available.…”

Section: Theorymentioning

confidence: 99%

Supplemental Carbon Use by Microorganisms Degrading Toxic Organic Compounds and the Concept of Specific Toxicity

Lindstrom¹,

Brown²

1989

Hazardous Waste and Hazardous Materials

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Biological treatment can be used to decontaminate soil and water polluted by toxic organic compounds. Some compounds are metabolized too slowly under natural conditions to be effectively removed from the contaminated system. The technical literature contains several examples where nutrient manipulation has been used to enhance microbial metabolism. One promising technique for increasing absolute biodégradation rates of organic toxins is to augment microbial biomass by adding a supplemental carbon substrate. Simultaneous metabolism of the toxic and supplemental substrates requires maintenance of growth limiting carbon concentrations in culture to avoid catabolite repression. Populations of microorganisms can metabolize very low levels of organic toxins at accelerated rates when an ancillary carbon source is supplied. Cell-specific degradation rates may remain unchanged, but absolute removal rates are increased by virtue of increased biomass. Ancillary carbon can also stabilize a population of microorganisms in the presence of high and otherwise inhibitory concentrations of organic toxins. In such cases where organic toxin levels are high and biomass is augmented by supplemental carbon a measure of toxicity is required which accounts for microbial population density as well as toxin concentration. This second order parameter is called specific toxicity.

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“…There are also findings for enhanced growth rates during growth on substrate mixtures in a discontinuous culture. For instance, Pseudomonas putida P3 is able to utilize glucose plus phenol simultaneously in a batch culture [54]. The maximum specific growth rate of P. putida P3 on phenol alone amounts to 0.1 h-' and on mixtures of glucose and phenol up to 0.41 h-', depending on the mixing ratio.…”

Section: Search For Bottlenecks Of' Growth Rates Nnd Their Expansionmentioning

confidence: 99%

The auxiliary substrate concept — an approach for overcoming limits of microbial performances

Babel

Brinkmann

Müller

1993

Acta Biotechnologica

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and OutlookIn biotechnological processes, fundamental performances of microorganisms are used. The economy of these processes is essentially determined by the efficiency, velocity (productivity) and quality of thc products. Therefore it is a permanent task and challenge for basic and biotechnological research to seek out measures for improving the actually attained parameters. The auxiliary substrate concept supplics an approach. It is based on the fact that chemo-organo-heterotrophic substrates differ in the carbon:energy ratio, thus, growth yield is limited in energy and/or reducing power. I t SAYS that. by simultaneous utilization of physiologically similar substrates (mixed substrates), the growth yield increases. The substrates are to combine in such a way that with their simultaneous utilization a minimum of carbon is dissimilated merely for the purpose of the generation of biologically useful energy and/or reducing power. Since all chemo-organo-heterotrophic substrates are more or less energy-deficient, an increase in growth efficiency can be expected if the individual substrates of the mixture are assimilated more efficiently than the respective substrates alone. This may result, for instance, from an immediate assimilation of a substrate (according to the "manner of finished part construction"). An increased growth rate is rather the rule than the exception in mixed substrate utilization.In product syntheses the substrates are. depending on the concrete product and metabolic pathway. either energy-excess or energy-deficient, or, in other words, the processes are energy-generating or energy-consuming, respectively. If this is responsible for discrepancies between the possible yields determined by the carbon metabolism and the experimentally obtained yields. the discrepancies should be able to be decreased and the yields increased by mixing substrates. The substrates are to choose and combine so that, due to simultaneous utilization, the product formation process becomes energy neutral. As a rule, the enhanced efficiency is accompanied by an increased velocity. This does not only apply to syntheses, but also to degradation (and detoxification) reactions. Even supposedly inert compounds or persistent substances can be activated by simultaneous (co-)metabolization of another (an auxiliary substrate, victim substrate or co-substrate) and converted at a considerable rate. It is of interest for syntheses of products but in particular for degradation and decontamination of harmful and waste products in the environment that the residual concentrations of the substrates are smaller than those achieved if the compounds of a mixture are metabolized separately. The auxiliary substrate concept has proven to be fruitful, both for theoretical and practical questions. It was practically already being used before it was formulated (mixed substrate utilization, cometabolism). However, an abundance of regulatory and energetic aspects are waiting to be investigated in more detail. 212Acta Biotechnologica 13 (1993) 3

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Regulation of the utilization of glucose and aromatic substrates in four strains of Pseudomonas putida

Cited by 22 publications

References 12 publications

Catabolite Effects On Enzyme Induction And Substrate Utilization In Rhizobium leguminosarum

Catabolite Effects On Enzyme Induction And Substrate Utilization In Rhizobium leguminosarum

Supplemental Carbon Use by Microorganisms Degrading Toxic Organic Compounds and the Concept of Specific Toxicity

The auxiliary substrate concept — an approach for overcoming limits of microbial performances

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