Regulation of the Transforming Growth Factor β-responsive Transcription Factor CTF-1 by Calcineurin and Calcium/ Calmodulin-dependent Protein Kinase IV

FGF18 Represses Noggin Expression and Is Induced by Calcineurin

“…Moreover, these calcium shifts were sufficient to activate calcineurin (47). Other published data show (48,49) that TGF-␤ can either stimulate or inhibit calcium mobilization and calcium signaling. Hence the effects of TGF-␤1 on calcium mobilization appear to be cell type-specific.…”

The repression of nuclear factor I/CCAAT transcription factor (NFI/CTF) transactivating domain by oxidative stress is mediated by a critical cysteine (Cys-427)

“…collagen gene) and repressed by tumour necrosis factor α and oxidative stress (thus mediating the CYP1A1 gene inhibition) [4,7,8]. The last 13 residues of NFI\CTF were identified as the TGFβ-responsive domain (TRD) [9].…”

“…The last 13 residues of NFI\CTF were identified as the TGFβ-responsive domain (TRD) [9]. In fibroblasts, the TGFβ effect is associated with an increase in cytosolic calcium [7]. In addition, overexpression of the small GTPase Ras or of the Raf-1 kinase represses NFI\CTF transcriptional activity through a mechanism that does not involve the phosphorylation of the TRD [8].…”

Calcineurin Regulates Nuclear Factor I Dephosphorylation and Activity in Malignant Glioma Cell Lines

“…The serine/threonine phosphatase calcineurin is expressed in neurons and reactive astrocytes (48,49) and has previously been associated with NFIC transactivation in NIH3T3 cells (41). We performed ChIP on U251 MG cells treated with the calcineurin inhibitor CsA to determine whether there was any effect on NFI occupancy of endogenous promoters.…”

“…As these neurons mature, binding of NFAT is temporally down-regulated resulting in an increase in NFI binding to target genes (40). A more direct link between calcineurin and NFI comes from the observation that calcineurin is able to activate the transactivation domain of NFIC in fibroblasts (41).…”