Regulation of the transcription factor E2F8 gene expression in bovine ovarian cells

Morrell, B.C.; Zhang, Lingna; Schütz, Luís F.; Perego, Maria Chiara; Maylem, Excel Rio S.; Spicer, L. J.

doi:10.1016/j.mce.2019.110572

Cited by 12 publications

(5 citation statements)

References 94 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…E2F8 was also expressed higher in other types of tumors and was closely related to tumor prognosis in preceding documents. 50 , 51 , 52 A study has shown that E2F8 was targeted by miR-223-5p, inhibiting tumor progression in non-small cell lung cancer (NSCLC). 53 Although the prognostic value of E2F8 has been studied in GC, 49 there was no definite and relevant study on the cellular mechanism of E2F8 on GC.…”

Section: Discussionmentioning

confidence: 99%

Novel Potential Therapeutic Target for E2F1 and Prognostic Factors of E2F1/2/3/5/7/8 in Human Gastric Cancer

Liu

2020

Molecular Therapy - Methods & Clinical Development

View full text Add to dashboard Cite

E2F transcription factors (E2Fs) were found to be related with cell activities and disease progression among a variety of different tumors, including regulating cell division and cell proliferation. In the analysis, it aimed to focus on transcriptional and survival information of E2Fs in gastric cancer (GC) from Gene Expression Profiling Interactive Analysis (GEPIA), Kaplan-Meier plotter, cBioPortal, Database for Annotation, Visualization and Integrated Discovery (DAVID), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, and Oncomine databases. It was found that the expression of E2F1/2/3/5/7/8 in GC tissues was obviously higher than the normal. Of interest, none of the E2Fs was related with pathological stages. Nevertheless, high expression of E2F2/3/5/7/8 was related with better survival data, except E2F6 regarding shorter first-progression (FP) survival. High expression levels of E2F2/5/7/8 have significant correlations with overall survival (OS) in patients with intestinal and diffuse GC, and this prognostic value is not affected by gender. Oppositely, the lower level of E2F1/4 illustrated superior survival data. Moreover, increased expression of E2F1 in GC tissues might play an important role in the development of GC. Collectively, E2F1 could be a potential therapeutic target for patients with GC. E2F1/2/3/5/7/8 might be original prognostic predictors of GC.

show abstract

Section: Discussionmentioning

confidence: 99%

Novel Potential Therapeutic Target for E2F1 and Prognostic Factors of E2F1/2/3/5/7/8 in Human Gastric Cancer

Liu

2020

Molecular Therapy - Methods & Clinical Development

View full text Add to dashboard Cite

show abstract

“…Ovaries from non-pregnant beef cows were collected from a local abattoir and based on surface diameter, GC was collected and prepared from small (1 to 5 mm) and large (8 to 22 mm) follicles as previously described [ 24 , 44 ]. The viability of GC from small and large follicles was determined by the trypan blue exclusion method and averaged 58 ± 7% and 64 ± 6%, respectively, and is similar to viabilities reported in previous studies [ 45 , 46 , 47 , 48 ].…”

Section: Methodsmentioning

confidence: 99%

In Vitro Effects of Enniatin A on Steroidogenesis and Proliferation of Bovine Granulosa Cells

Chiminelli

Spicer

Maylem

et al. 2022

Toxins

View full text Add to dashboard Cite

The emerging Fusarium mycotoxins enniatins (ENNs) have been the focus of new research because of their well-documented existence in various cereal and grain products. Research findings indicate that reproductive disorders may be caused by exposure to Fusarium mycotoxins, but little work has evaluated ENNs on reproductive function. Therefore, to determine the effects of ENNA on the proliferation and steroidogenesis of granulosa cells (GC), experiments were conducted using bovine GC cultures. In vitro, ENNA (1–5 μM) inhibited (p < 0.05) hormone-induced GC progesterone and estradiol production. The inhibitory effect of ENNA on estradiol production was more pronounced in small- than large-follicle GC. In large-follicle GC, 0.3 μM ENNA had no effect (p > 0.10) whereas 1 and 3 μM ENNA inhibited GC proliferation. In small-follicle GC, ENNA (1–5 μM) dramatically decreased (p < 0.05) GC proliferation. Using cell number data, the IC50 of ENNA was estimated at 2 μM for both follicle sizes. We conclude that ENNA can directly inhibit ovarian function in cattle, decreasing the proliferation and steroid production of GC.

show abstract

“…54 Elegant works from a number of laboratories have showed E2F family members are transcription regulators of RAD51, such as E2F1, E2F4, and E2F7. [55][56][57] There is limited research on the function of E2F8 in the ovary 58 and the activation mechanism of IGF-I on HR repair under hypoxic condition is also unclear. To investigate whether E2F8 participates in IGF-I-mediated HR repair in GCs, we first detected the mRNA and protein levels of E2F8 with the treatment of hypoxia and/or IGF-I.…”

Section: Discussionmentioning

confidence: 99%

IGF‐I protects porcine granulosa cells from hypoxia‐induced apoptosis by promoting homologous recombination repair through the PI3K/AKT/E2F8/RAD51 pathway

Liu,

Feng,

et al. 2023

The FASEB Journal

View full text Add to dashboard Cite

Severe hypoxia induced by vascular compromise (ovarian torsion, surgery), obliteration of vessels (aging, chemotherapy, particularly platinum drugs) can cause massive follicle atresia. On the other hand, hypoxia increases the occurrence of DNA double‐strand breaks (DSBs) and triggers cellular damage repair mechanisms; however, if the damage is not promptly repaired, it can also induce the apoptosis program. Insulin‐like growth factor‐I (IGF‐I) is a polypeptide hormone that plays essential roles in stimulating mammalian follicular development. Here, we report a novel role for IGF‐I in protecting hypoxic GCs from apoptosis by promoting DNA repair through the homologous recombination (HR) process. Indeed, the hypoxic environment within follicles significantly inhibited the efficiency of HR‐directed DNA repair. The presence of IGF‐I‐induced HR pathway to alleviate hypoxia‐induced DNA damage and apoptosis primarily through upregulating the expression of the RAD51 recombinase. Importantly, we identified a new transcriptional regulator of RAD51, namely E2F8, which mediates the protective effects of IGF‐I on hypoxic GCs by facilitating the transcriptional activation of RAD51. Furthermore, we demonstrated that the PI3K/AKT pathway is crucial for IGF‐I‐induced E2F8 expression, resulting in increased RAD51 expression and enhanced HR activity, which mitigates hypoxia‐induced DNA damage and thereby protects against GCs apoptosis. Together, these findings define a novel mechanism of IGF‐I‐mediated GCs protection by activating the HR repair through the PI3K/AKT/E2F8/RAD51 pathway under hypoxia.

show abstract

Regulation of the transcription factor E2F8 gene expression in bovine ovarian cells

Cited by 12 publications

References 94 publications

Novel Potential Therapeutic Target for E2F1 and Prognostic Factors of E2F1/2/3/5/7/8 in Human Gastric Cancer

Novel Potential Therapeutic Target for E2F1 and Prognostic Factors of E2F1/2/3/5/7/8 in Human Gastric Cancer

In Vitro Effects of Enniatin A on Steroidogenesis and Proliferation of Bovine Granulosa Cells

IGF‐I protects porcine granulosa cells from hypoxia‐induced apoptosis by promoting homologous recombination repair through the PI3K/AKT/E2F8/RAD51 pathway

Contact Info

Product

Resources

About