Regulation of the regulator of G protein signaling 2 expression and cellular localization by PKA and PKC pathways in mouse granulosa cells

Yeh, Hsiao Yu; Sun, David; Peng, Yen Chun; Wu, Yuh Lin

doi:10.1016/j.bbrc.2018.06.101

Cited by 3 publications

(4 citation statements)

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“…The transcriptome factors prediction showed that the binding sites of Sp1, NF‐1 and Oct‐1 were changed after mutation. Yeh et al (2018) discovered that PKA‐ and PKC‐mediated RGS2 transcription in granulosa cells was in a Sp‐1‐dependent manner. Tsai‐Morris et al (1994) illustrated that NF‐1 was related to the control of luteinizing hormone receptor gene expression in gonadal cells.…”

Section: Discussionmentioning

confidence: 99%

“…ers, and two SNPs (SNP1 and SNP3) were significantly affected the average litter size in Bama Xiang pigs. The transcriptome factors prediction showed that the binding sites of Sp1, NF-1 and Oct-1 were changed after mutation Yeh et al (2018). discovered that PKA-and PKC-mediated RGS2 transcription in granulosa cells was in a Sp-1dependent manner Tsai-Morris et al (1994).…”

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confidence: 99%

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Identifying selection signatures for litter size in Guangxi Bama Xiang pigs

GangYan

et al. 2022

Reprod Domestic Animals

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Litter size is an important economic trait in pig production. However, the genetic mechanisms underlying varying litter size in Guangxi Bama Xiang pigs remain unknown. To identify selection signatures for litter size in Guangxi Bama Xiang pigs, we obtained 297 Illumina PorcineSNP50 BeadChip array data and the average born number (ABN) from parity one to nine in Guangxi Bama Xiang pigs. Fixation index (Fst) methods were used to identify the selection signature of the litter size, and three phenotypic gradient differential population pairs (according to the ABN) in individuals were used to reduce the false positives of signature selections. Single nucleotide polymorphisms (SNPs) were identified in the VEGFA promoter and exons. The general linear model was used to analyse the differences in distinct genotypes after they were typed using three‐round multiplex PCR technology. Finally, the transcriptome factor and CpG island in the VEGFA promoter were predicted. A total of 328, 328 and 317 significant loci were identified in the 1st, 2nd and 3rd population pairs, respectively. After removing the false positives, 25 SNPs were defined as the selection signatures in relation to litter size. Ten (VEGFA, USP49, USP25, SRPK1, SLC26A8, RPL10A, PPARD, MAPK14, HMGA1 and CHRDL2) out of 52 genes in the selection regions were annotated as the candidate genes of litter size, respectively, VEGFA. There were no SNPs in the VEGFA exon region, but we obtained three SNPs (rs786889605, rs343769603 and rs323942424) in the VEGFA promoter regions. The ABN in CC was significantly higher than that in TT in rs786889605, and the ABN in TT was significantly lower than that in GG in rs323942424. Meanwhile, the mutation of the VEGFA promoter result in the loss of Sp1 and NF‐1 and the formation of Oct‐1. In summary, we obtained ten candidate genes, and two mutations in the VEGFA promoter that could be important potential molecular biomarkers for litter size in Bama Xiang pigs.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Identifying selection signatures for litter size in Guangxi Bama Xiang pigs

GangYan

et al. 2022

Reprod Domestic Animals

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“…Notably, some transcription factors, such as Sp1, BRCA1, and ELK1, are involved in female reproduction, similar to BMPR1B [17]. Sp1 belongs to the Sp/KLF family of transcription factors, which is essential for ovarian functions by regulating known female reproduction-related genes, such as CYP11A1, CYP17 , IRS-2 , and RGS , by direct binding to a consensus GC-rich motif within the promoter of these targets, in mammalian ovary [18,19,20,21]. BRCA1 is known as a tumor suppressor gene for breast and ovarian cancer, and belongs to the family of ataxia-telangiectasia-mutated-mediated DNA double-strand break (DSB) repair genes and plays a critical role in homology-directed repair of DSBs [22].…”

Section: Discussionmentioning

confidence: 99%

Smad4 Feedback Enhances BMPR1B Transcription in Ovine Granulosa Cells

Abdurahman

Yao

et al. 2019

IJMS

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BMPR1B is a type 1B receptor of the canonical bone morphogenetic protein (BMP)/Sma- and mad-related protein (Smad) signaling pathway and is well known as the first major gene associated with sheep prolificacy. However, little is known about the transcriptional regulation of the ovine BMPR1B gene. In this study, we identified the ovine BMPR1B gene promoter and demonstrated that its transcription was regulated by Smad4. In sheep ovarian follicles, three transcriptional variants of BMPR1B gene with distinct transcription start sites were identified using 5′ RACE assay while variants II and III were more strongly expressed. Luciferase assay showed that the region −405 to −200 nt is the PII promoter region of variant II. Interestingly, two putative Smad4-binding elements (SBEs) were detected in this region. Luciferase and ChIP assay revealed that Smad4 enhances PII promoter activity of the ovine BMPR1B gene by directly interacting with SBE1 motif. Furthermore, in the ovine granulosa cells, Smad4 regulated BMPRIB expression, and BMPRIB-mediated granulosa cells apoptosis. Overall, our findings not only characterized the 5’ regulatory region of the ovine BMPR1B gene, but also uncovered a feedback regulatory mechanism of the canonical BMP/Smad signaling pathway and provided an insight into the transcriptional regulation of BMPR1B gene and sheep prolificacy.

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“…In the list of palmitoyl-proteins, there are numerous G-proteins and GPCRs, secreted proteins, receptors of estrogen, growth factors, immune receptors and other proteins [ 9 , 18 , 19 ]. Many proteins, which were found palmitoylated in different tissues, are largely involved in female reproduction through their activity in the ovarian follicular cells and enclosed oocytes [ 20 , 21 , 22 ], although no data on palmitoylation of these proteins in the ovarian cells in mammals were reported yet. In Xenopus, palmitoylation of H-Ras protein and G-protein subunit alpha (GNAS) are involved in oocyte meiotic arrest [ 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

Protein Palmitoylation in Bovine Ovarian Follicle

Uzbekova

Teixeira-Gomes

Marestaing

et al. 2021

IJMS

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Protein palmitoylation is a reversible post-translational modification by fatty acids (FA), mainly a palmitate (C16:0). Palmitoylation allows protein shuttling between the plasma membrane and cytosol to regulate protein stability, sorting and signaling activity and its deficiency leads to diseases. We aimed to characterize the palmitoyl-proteome of ovarian follicular cells and molecular machinery regulating protein palmitoylation within the follicle. For the first time, 84 palmitoylated proteins were identified from bovine granulosa cells (GC), cumulus cells (CC) and oocytes by acyl-biotin exchange proteomics. Of these, 32 were transmembrane proteins and 27 proteins were detected in bovine follicular fluid extracellular vesicles (ffEVs). Expression of palmitoylation and depalmitoylation enzymes as palmitoyltransferases (ZDHHCs), acylthioesterases (LYPLA1 and LYPLA2) and palmitoylthioesterases (PPT1 and PPT2) were analysed using transcriptome and proteome data in oocytes, CC and GC. By immunofluorescence, ZDHHC16, PPT1, PPT2 and LYPLA2 proteins were localized in GC, CC and oocyte. In oocyte and CC, abundance of palmitoylation-related enzymes significantly varied during oocyte maturation. These variations and the involvement of identified palmitoyl-proteins in oxidation-reduction processes, energy metabolism, protein localization, vesicle-mediated transport, response to stress, G-protein mediated and other signaling pathways suggests that protein palmitoylation may play important roles in oocyte maturation and ffEV-mediated communications within the follicle.

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Regulation of the regulator of G protein signaling 2 expression and cellular localization by PKA and PKC pathways in mouse granulosa cells

Cited by 3 publications

References 30 publications

Identifying selection signatures for litter size in Guangxi Bama Xiang pigs

Identifying selection signatures for litter size in Guangxi Bama Xiang pigs

Smad4 Feedback Enhances BMPR1B Transcription in Ovine Granulosa Cells

Protein Palmitoylation in Bovine Ovarian Follicle

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