Regulation of the plasma membrane during exposure to low temperatures in suspension-cultured cells from a cryophyte (Chorispora bungeana)

Shi, Yulan; An, Lizhe; Zhang, Manxiao; Huang, Caihong; Zhang, Hua; Xu, Shou‐Jun

doi:10.1007/s00709-008-0291-1

Cited by 23 publications

(35 citation statements)

References 31 publications

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“…To adapt to low temperature environment, many physiological and molecular changes occur during cold acclimation (Thomashow, 1999). Exposure to low temperature, linolenic acid and membrane lipid unsaturation increased, and the plasma membrane H + -ATPase activity increased and these changes are essential for the plants to withstand low temperature (Shi et al, 2008). In addition, calcium-dependent protein kinase confers cold tolerance via the regulation of calcium channel in plasma membrane (Xiong et al, 2002; Komatsu et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Analysis of Gene Expression Provides New Insights into Cold Responses in Thellungiella salsuginea

et al. 2017

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Low temperature is one of the major environmental stresses that affects plant growth and development, and leads to decrease in crop yield and quality. Thellungiella salsuginea (salt cress) exhibits high tolerance to chilling, is an appropriate model to investigate the molecular mechanisms of cold tolerance. Here, we compared transcription changes in the roots and leaves of T. salsuginea under cold stress using RNA-seq. We identified 2,782 and 1,430 differentially expressed genes (DEGs) in leaves and roots upon cold treatment, respectively. The expression levels of some genes were validated by quantitative real-time-PCR (qRT-PCR). Among these DEGs, 159 (11.1%) genes in roots and 232 (8.3%) genes in leaves were annotated as various types of transcription factors. We found that five aquaporin genes (three TIPs, one PIPs, and one NIPs) responded to cold treatment. In addition, the expression of COR47, ICE1, and CBF1 genes of DREB1/CBF-dependent cold signaling pathway genes altered in response to low temperature. KEGG pathway analysis indicated that these cold regulated genes were enriched in metabolism, photosynthesis, circadian rhythm, and transcriptional regulation. Our findings provided a complete picture of the regulatory network of cold stress response in T. salsuginea. These cold-responsive genes could be targeted for detail functional study and utilization in crop cold tolerance improvement.

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Section: Introductionmentioning

confidence: 99%

Genome-Wide Analysis of Gene Expression Provides New Insights into Cold Responses in Thellungiella salsuginea

et al. 2017

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“…Our previous studies showed that the callus and suspension cells from C. bungeana were ready to endure freezing temperature (−4°C) without cold acclimation [3,6]. The aim of this study is to examine what kinds of mechanisms contribute to the specific cold tolerance of C. bungeana .…”

Section: Introductionmentioning

confidence: 99%

Deep-sequencing transcriptome analysis of chilling tolerance mechanisms of a subnival alpine plant, Chorispora bungeana

et al. 2012

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BackgroundThe plant tolerance mechanisms to low temperature have been studied extensively in the model plant Arabidopsis at the transcriptional level. However, few studies were carried out in plants with strong inherited cold tolerance. Chorispora bungeana is a subnival alpine plant possessing strong cold tolerance mechanisms. To get a deeper insight into its cold tolerance mechanisms, the transcriptome profiles of chilling-treated C. bungeana seedlings were analyzed by Illumina deep-sequencing and compared with Arabidopsis.ResultsTwo cDNA libraries constructed from mRNAs of control and chilling-treated seedlings were sequenced by Illumina technology. A total of 54,870 unigenes were obtained by de novo assembly, and 3,484 chilling up-regulated and 4,571 down-regulated unigenes were identified. The expressions of 18 out of top 20 up-regulated unigenes were confirmed by qPCR analysis. Functional network analysis of the up-regulated genes revealed some common biological processes, including cold responses, and molecular functions in C. bungeana and Arabidopsis responding to chilling. Karrikins were found as new plant growth regulators involved in chilling responses of C. bungeana and Arabidopsis. However, genes involved in cold acclimation were enriched in chilling up-regulated genes in Arabidopsis but not in C. bungeana. In addition, although transcription activations were stimulated in both C. bungeana and Arabidopsis, no CBF putative ortholog was up-regulated in C. bungeana while CBF2 and CBF3 were chilling up-regulated in Arabidopsis. On the other hand, up-regulated genes related to protein phosphorylation and auto-ubiquitination processes were over-represented in C. bungeana but not in Arabidopsis.ConclusionsWe conducted the first deep-sequencing transcriptome profiling and chilling stress regulatory network analysis of C. bungeana, a subnival alpine plant with inherited cold tolerance. Comparative transcriptome analysis suggests that cold acclimation is not a major chilling tolerance mechanism of C. bungeana. Activation of protein phosphorylation and ubiquitination may confer chilling tolerance to C. bungeana in a more rapid and flexible way than cold acclimation. Such differences may have contributed to the differences in cold tolerance between C. bungeana and Arabidopsis. The results presented in this paper will be informative for gene discovery and the molecular mechanisms related to plant cold tolerance.

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“…Likewise, PM H ? -ATPase activity increased during cold treatment exposure and finally displayed similar activity to the enzyme maintained at 25°C (Shi et al 2008). These findings suggest that this cryophyte adapts its plasma membrane in order to withstand low temperatures.…”

Section: Interaction With Sterols: the Planar Solutionmentioning

confidence: 59%

“…Changes in H ? -ATPase activity have also been reported (Jian et al 1982;Ishikawa and Yoshida, 1985;White et al 1990;Martz et al 2006;Zhang et al 2006;Shi et al 2008;Janicka-Russak et al 2012;Kim et al 2013;Oh et al 2014;Zhou et al 2014). The mechanisms behind these changes are not yet known in detail, but cold acclimation seems to be associated with modifications of the membrane lipid composition and their effects on the PM H ?…”

Section: Interaction With Glycerolipids: the Real World Of The Membramentioning

confidence: 71%

“…Exposure of plants to cold temperatures has been associated with changes in lipid composition and unsaturation of FA, presumably of the glycerolipid fraction, but freezing promotes the formation of nonbilayer hexagonal II phase in the membranes (Lynch and Steponkus 1987;White et al 1990;Uemura et al 1995;Welti et al 2002;Martz et al 2006;Shi et al 2008). Changes in H ?…”

Section: Interaction With Glycerolipids: the Real World Of The Membramentioning

confidence: 97%

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Plant lipid environment and membrane enzymes: the case of the plasma membrane H+-ATPase

et al. 2015

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Several lipid classes constitute the universal matrix of the biological membranes. With their amphipathic nature, lipids not only build the continuous barrier that confers identity to every cell and organelle, but they are also active actors that modulate the activity of the proteins immersed in the lipid bilayer. The plasma membrane H(+)-ATPase, an enzyme from plant cells, is an excellent example of a transmembrane protein whose activity is influenced by the hydrophilic compartments at both sides of the membrane and by the hydrophobic domains of the lipid bilayer. As a result, an extensive documentation of the effect of numerous amphiphiles in the enzyme activity can be found. Detergents, membrane glycerolipids, and sterols can produce activation or inhibition of the enzyme activity. In some cases, these effects are associated with the lipids of the membrane bulk, but in others, a direct interaction of the lipid with the protein is involved. This review gives an account of reports related to the action of the membrane lipids on the H(+)-ATPase activity.

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Regulation of the plasma membrane during exposure to low temperatures in suspension-cultured cells from a cryophyte (Chorispora bungeana)

Cited by 23 publications

References 31 publications

Genome-Wide Analysis of Gene Expression Provides New Insights into Cold Responses in Thellungiella salsuginea

Genome-Wide Analysis of Gene Expression Provides New Insights into Cold Responses in Thellungiella salsuginea

Deep-sequencing transcriptome analysis of chilling tolerance mechanisms of a subnival alpine plant, Chorispora bungeana

Plant lipid environment and membrane enzymes: the case of the plasma membrane H+-ATPase

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