Regulation of the nah and sal operons of plasmid NAH7: Evidence for a new function in nahR

You, In Soon; Gunsalus, I. C.

doi:10.1016/s0006-291x(86)80141-3

Cited by 10 publications

(13 citation statements)

References 16 publications

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“…A putative ribosomebinding site (RBS) complementary to the 3' end of the 16S rRNA of Pseudomonas aeruginosa (26) was found 5 bp upstream from the start ATG codon. The nahR complementation (34) and the maxicell data presented here, as obtained from the several nahR clones, support the 1,122-bp ORF as the nahR coding sequence. A second ORF was found within this long ORF; it started at position 481 and was preceded by a putative RBS (Fig.…”

Section: Methodssupporting

confidence: 74%

“…Promoter (Piac) orientations in the pUC vectors (31) RESULTS nahR region, subclones, and restriction analysis. In trans, plasmid pY1317 complements the five regulatory mutations nahR201 to nahR205 to Nah+ Sal', whereas pY1342 restores only two, nahR203 and nahR204, to wild type (34). To further dissect the nahR region, subclones were generated in Characterization and stability of polypeptides expressed in E. coli from nahR-region clones.…”

Section: Methodsmentioning

confidence: 99%

“…The expression of the nah and sal operons is regulated positively by nahR in the presence of salicylate (34). The basicity of the predicted amino acid sequence of the nahR gene product suggests a DNA-binding protein.…”

Section: Methodsmentioning

confidence: 99%

“…The nah operon (nahABCDEF) encodes enzymes for the conversion of naphthalene to salicylate, and the sal operon (nahG HINLJK) encodes enzymes for the oxidation of salicylate to pyruvate and acetaldehyde via the meta-cleavage pathway (9,32). Both operons are under positive control by salicylate (2,32) through the regulator nahR (8,33,34). The structural genes are ordered in a sequence similar to that of the enzymatic steps and are transcribed in one direction, whereas the regulator locus nahR, just upstream of the sal operon, is transcribed in the opposite orientation (Fig.…”

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confidence: 99%

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Nucleotide sequence of plasmid NAH7 gene nahR and DNA binding of the nahR product

1988

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The nah and sal operons of the 80-kilobase-pair (kb) NAH7 plasmid specify catabolism of naphthalene and salicylate under positive regulation by gene nahR. A 1.75-kb fragment (PstI-HindIll) In Pseudomonas putida, the genes specifying naphthalene catabolism are organized into two operons, nah and sal, and are carried on an 80-kilobase-pair (kb) plasmid, NAH7. The nah operon (nahABCDEF) encodes enzymes for the conversion of naphthalene to salicylate, and the sal operon (nahG HINLJK) encodes enzymes for the oxidation of salicylate to pyruvate and acetaldehyde via the meta-cleavage pathway (9, 32). Both operons are under positive control by salicylate (2, 32) through the regulator nahR (8,33,34). The structural genes are ordered in a sequence similar to that of the enzymatic steps and are transcribed in one direction, whereas the regulator locus nahR, just upstream of the sal operon, is transcribed in the opposite orientation (Fig. 1). The slightly larger TOL plasmid pWWO specifies the oxidative enzymes for toluene by a similar catabolic aromatic pathway which is also in two operons under positive control but regulated by two genes, xylR and xylS (4,5,(10)(11)(12)(13)18).The molecular details of positive regulation, in part elucidated by a single regulator, are not as well understood as the carefully studied details of repressor-mediated negative regulation by the tryptophan operon or those of the twocomponent positive and negative regulation of bacteriophage lambda lysogeny by the cI and cro genes. A positiveregulation model for the catabolic plasmids is the TOL plasmid pWWO, in which xyl operons are controlled by two regulatory genes, xylR and xylS, both mapped distal to catabolic genes. The expression of gene xylS is positively controlled by the xylR gene product, which in turn autoregulates the expression of the xylR gene (13,22,27

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Section: Methodssupporting

confidence: 74%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Nucleotide sequence of plasmid NAH7 gene nahR and DNA binding of the nahR product

1988

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“…Although most information on naphthalene catabolism has been based on the analysis of NAH7 (12,16,37,66,68,69), until now the complete nucleotide sequence of this IncP-9 plasmid has not been reported. In addition, further detailed molecular features have remained unclear with respect to the unique site-specific resolution system of Tn4655.…”

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confidence: 99%

Genomic and Functional Analysis of the IncP-9 Naphthalene-Catabolic Plasmid NAH7 and Its Transposon Tn 4655 Suggests Catabolic Gene Spread by a Tyrosine Recombinase

et al. 2006

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The naphthalene-catabolic (nah) genes on the incompatibility group P-9 (IncP-9) self-transmissible plasmid NAH7 from Pseudomonas putida G7 are some of the most extensively characterized genetic determinants for bacterial aerobic catabolism of aromatic hydrocarbons. In contrast to the detailed studies of its catabolic cascade and enzymatic functions, the biological characteristics of plasmid NAH7 have remained unclear. Our sequence determination in this study together with the previously deposited sequences revealed the entire structure of NAH7 (82,232 bp). Comparison of NAH7 with two other completely sequenced IncP-9 catabolic plasmids, pDTG1 and pWW0, revealed that the three plasmids share very high nucleotide similarities in a 39-kb region encoding the basic plasmid functions (the IncP-9 backbone). The backbone of NAH7 is phylogenetically more related to that of pDTG1 than that of pWW0. These three plasmids carry their catabolic gene clusters at different positions on the IncP-9 backbone. All of the NAH7-specified nah genes are located on a class II transposon, Tn4655. Our analysis of the Tn4655-encoded site-specific recombination system revealed that (i) a novel tyrosine recombinase, TnpI, catalyzed both the intra-and intermolecular recombination between two copies of the attI site, (ii) the functional attI site was located within a 119-bp segment, and (iii) the site-specific strand exchange occurred within a 30-bp segment in the 41-bp CORE site. Our results and the sequence data of other naphthalene-catabolic plasmids, pDTG1 and pND6-1, suggest a potential role of the TnpI-attI recombination system in the establishment of these catabolic plasmids.

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The physiology of aromatic hydrocarbon degrading bacteria

Smith

1994

Biochemistry of Microbial Degradation

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Regulation of the nah and sal operons of plasmid NAH7: Evidence for a new function in nahR

Cited by 10 publications

References 16 publications

Nucleotide sequence of plasmid NAH7 gene nahR and DNA binding of the nahR product

Nucleotide sequence of plasmid NAH7 gene nahR and DNA binding of the nahR product

Genomic and Functional Analysis of the IncP-9 Naphthalene-Catabolic Plasmid NAH7 and Its Transposon Tn 4655 Suggests Catabolic Gene Spread by a Tyrosine Recombinase

The physiology of aromatic hydrocarbon degrading bacteria

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