Regulation of the NADH supply by nuoE deletion and pncB overexpression to enhance hydrogen production in Enterobacter aerogenes

Jiang, Ke; Li, Li; Liu, Shuxin; Xu, Yizhuang; Yang, Jiayao; Chu, Wanying; Lü, Ping; Gao, Ting; Bai, Ruoxuan; Xu, Fangxu; Zhao, Hongxin

doi:10.1016/j.ijhydene.2022.02.096

Cited by 13 publications

(3 citation statements)

References 44 publications

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“…Nicotinate phosphoribosyl transferase is the rate-limiting enzyme of the NAD(H) synthesis system and can catalyze the conversion of niacin (NA) to nicotinic acid mononucleotide (NAMN), which is an intermediate of the NAD + synthesis pathway (Ma et al, 2013). The upregulation of pncB can increase the NAD(H) pool and enhance the redox level of microbial cells to reduce Cr(VI) (Jiang et al, 2022). However, the toxic effects of Cr(VI) on cellular metabolism were more pronounced.…”

Section: Cell Envelopementioning

confidence: 99%

Physiological and transcriptional studies reveal Cr(VI) reduction mechanisms in the exoelectrogen Cellulomonas fimi Clb-11

Cao

Zhao

et al. 2023

Front. Microbiol.

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A facultative exoelectrogen, Cellulomonas fimi strain Clb-11, was isolated from polluted river water. This strain could generate electricity in microbial fuel cells (MFCs) with carboxymethyl cellulose (CMC) as the carbon source, and the maximum output power density was 12.17 ± 2.74 mW·m−2. In addition, Clb-11 could secrete extracellular chromate reductase or extracellular electron mediator to reduce Cr(VI) to Cr(III). When the Cr(VI) concentration was less than 0.5 mM in Luria-Bertani (LB) medium, Cr(VI) could be completely reduced by Clb-11. However, the Clb-11 cells swelled significantly in the presence of Cr(VI). We employed transcriptome sequencing analysis to identify genes involved in different Cr(VI) stress responses in Clb-11. The results indicate that 99 genes were continuously upregulated while 78 genes were continuously downregulated as the Cr(VI) concentration increased in the growth medium. These genes were mostly associated with DNA replication and repair, biosynthesis of secondary metabolites, ABC transporters, amino sugar and nucleotide sugar metabolism, and carbon metabolism. The swelling of Clb-11 cells might have been related to the upregulation of the genes atoB, INO1, dhaM, dhal, dhak, and bccA, which encode acetyl-CoA C-acetyltransferase, myo-inositol-1-phosphate synthase, phosphoenolpyruvate-glycerone phosphotransferase, and acetyl-CoA/propionyl-CoA carboxylase, respectively. Interestingly, the genes cydA and cydB related to electron transport were continuously downregulated as the Cr(VI) concentration increased. Our results provide clues to the molecular mechanism of Cr(VI) reduction by microorganisms in MFCs systems.

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Section: Cell Envelopementioning

confidence: 99%

Physiological and transcriptional studies reveal Cr(VI) reduction mechanisms in the exoelectrogen Cellulomonas fimi Clb-11

Cao

Zhao

et al. 2023

Front. Microbiol.

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“…First, Nampt is used as the biocatalyst, and nicotinamide and 5′-phosphoribosyl-1′-pyrophosphate (PRPP) are used as the substrate to synthesize NMN. The second is the synthesis of nicotinic mononucleotide catalyzed by nicotinic acid phosphotransferase to form NMN through NAD synthase . The last one is that nicotinamide ribose (NR) generates NMN in response to nicotinamide riboside kinase (NRK) …”

Section: Introductionmentioning

confidence: 99%

“…The second is the synthesis of nicotinic mononucleotide catalyzed by nicotinic acid phosphotransferase to form NMN through NAD synthase. 19 The last one is that nicotinamide ribose (NR) generates NMN in response to nicotinamide riboside kinase (NRK). 20 Each of the three methods has its own set of advantages and disadvantages.…”

Section: ■ Introductionmentioning

confidence: 99%

Streamlining Design, Engineering, and Applications of Nicotinamide Riboside Kinase for Sustainable Biosynthesis of Nicotinamide Mononucleotide in Flow

Cheng,

Wu,

et al. 2023

ACS Sustainable Chem. Eng.

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The market demand for β-nicotinamide mononucleotide (NMN), an antiaging health product, is rapidly expanding. Consequently, there is an urgent requirement for an environment-friendly and economical approach to synthesize NMN. In this study, we develop a biocatalytic synthesis method for the production of valuable NMN from chemically synthesized nicotinamide riboside (NR). To enhance the efficiency of the process, the biocatalyst human nicotinamide riboside kinase (HS-NRK) was engineered by introducing a beneficial substitution (G13S) near the ATP-binding site. As a result, a 100% conversion of 50 g L −1 NR was achieved by using the engineered variant NRK G13S . However, the NMN yield was limited to 78% (39 g L −1 ) due to biodegradation by endogenous enzymes present in Escherichia coli. To overcome this challenge, we identified and knocked out the deoD gene for NMN biodegradation. Through protein engineering and metabolic engineering, we achieved a high NMN yield of 93% within 2 h using an effective whole-cell biocatalysis approach. Finally, an immobilized biocatalyst in continuous operation was developed, which facilitated the enzymatic process and enabled the cost-effective and environmentally attractive production of NMN.

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Microalgae-derived hydrogen production towards low carbon emissions via large-scale outdoor systems

Sung

Yang

et al. 2022

Bioresource Technology

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Regulation of the NADH supply by nuoE deletion and pncB overexpression to enhance hydrogen production in Enterobacter aerogenes

Cited by 13 publications

References 44 publications

Physiological and transcriptional studies reveal Cr(VI) reduction mechanisms in the exoelectrogen Cellulomonas fimi Clb-11

Physiological and transcriptional studies reveal Cr(VI) reduction mechanisms in the exoelectrogen Cellulomonas fimi Clb-11

Streamlining Design, Engineering, and Applications of Nicotinamide Riboside Kinase for Sustainable Biosynthesis of Nicotinamide Mononucleotide in Flow

Microalgae-derived hydrogen production towards low carbon emissions via large-scale outdoor systems

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