Regulation of the lignocellulolytic response in filamentous fungi

Huberman, Lori B.; Liu, Jason; Qin, Lu‐Ping; Glass, N. Louise

doi:10.1016/j.fbr.2016.06.001

Cited by 80 publications

(71 citation statements)

References 103 publications

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“…Our data indicate that CLR-3 acts to repress the activity of the transcription factor CLR-1 in the absence of an inducer. Although homologs of clr-1 occur throughout ascomycete species, the role of CLR-1 as the regulator of the cellulolytic response does not appear to be broadly conserved (3). Thus, we hypothesize that functional orthologs of clr-3 may exist only in species in which CLR-1 regulates the cellulolytic response, consistent with the conservation of CLR-3 among sordariomycete species (Fig.…”

Section: Discussionsupporting

confidence: 53%

“…In fungi that can utilize cellulose, lignocellulolytic gene expression is repressed when preferred carbon sources are present through a process known as "carbon catabolite repression" (11). There are several transcription factors involved in carbon catabolite repression (3). The best studied is the zinc finger transcription factor cre-1 (NCU08807), the N. crassa ortholog of Saccharomyces cerevisiae MIG1 (12,13).…”

mentioning

confidence: 99%

“…Utilization of these complex carbohydrates requires the cell to activate expression of genes encoding secreted enzymes that degrade insoluble carbohydrates into sugars that can be subsequently imported into the cell (3). If these enzymes are not produced, the cell cannot utilize these complex carbon sources (4)(5)(6).…”

mentioning

confidence: 99%

“…Mutations in nutrient-sensing pathways also play a role in cancer progression, since rapid growth of tumors causes physiological changes that result in abnormal nutrient requirements and utilization (2). In fungi, inaccurate nutrient sensing and signaling can result in slow growth or an inability to appropriately utilize nutrients in the environment (3).…”

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confidence: 99%

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Network of nutrient-sensing pathways and a conserved kinase cascade integrate osmolarity and carbon sensing in Neurospora crassa

Huberman

Coradetti

Glass

2017

Proc. Natl. Acad. Sci. U.S.A.

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Identifying nutrients available in the environment and utilizing them in the most efficient manner is a challenge common to all organisms. The model filamentous fungus Neurospora crassa is capable of utilizing a variety of carbohydrates, from simple sugars to the complex carbohydrates found in plant cell walls. The zinc binuclear cluster transcription factor CLR-1 is necessary for utilization of cellulose, a major, recalcitrant component of the plant cell wall; however, expression of clr-1 in the absence of an inducer is not sufficient to induce cellulase gene expression. We performed a screen for unidentified actors in the cellulose-response pathway and identified a gene encoding a hypothetical protein (clr-3) that is required for repression of CLR-1 activity in the absence of an inducer. Using clr-3 mutants, we implicated the hyperosmotic-response pathway in the tunable regulation of glycosyl hydrolase production in response to changes in osmolarity. The role of the hyperosmotic-response pathway in nutrient sensing may indicate that cells use osmolarity as a proxy for the presence of free sugar in their environment. These signaling pathways form a nutrient-sensing network that allows N. crassa cells to tightly regulate gene expression in response to environmental conditions. MAP kinase cascade | carbon metabolism | hyperosmotic response | carbon catabolite repression | plant biomass degradation

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Section: Discussionsupporting

confidence: 53%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Network of nutrient-sensing pathways and a conserved kinase cascade integrate osmolarity and carbon sensing in Neurospora crassa

Huberman

Coradetti

Glass

2017

Proc. Natl. Acad. Sci. U.S.A.

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“…Neste sistema, a concentração de monossacarídeos é detectada por sensores de glicose (proteína quinase cAMP) e, quando em concentração suficiente, o fator de transcrição CreA, localizado no núcleo, causa a repressão de genes alvos (HUBERMAN et al, 2016).…”

Section: Parâmetro De Bioprocesso: Fonte De Carbonounclassified

Estudo da alteração do perfil de proteínas de fungos filamentosos em diferentes condições de cultivo utilizando ferramentas proteômicas e ensaios enzimáticos

Garzon¹

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The pathway intermediate 2‐keto‐3‐deoxy‐L‐galactonate mediates the induction of genes involved in D‐galacturonic acid utilization in Aspergillus niger

et al. 2017

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In Aspergillus niger, the enzymes encoded by gaaA, gaaB, and gaaC catabolize d‐galacturonic acid (GA) consecutively into l‐galactonate, 2‐keto‐3‐deoxy‐l‐galactonate, pyruvate, and l‐glyceraldehyde, while GaaD converts l‐glyceraldehyde to glycerol. Deletion of gaaB or gaaC results in severely impaired growth on GA and accumulation of l‐galactonate and 2‐keto‐3‐deoxy‐l‐galactonate, respectively. Expression levels of GA‐responsive genes are specifically elevated in the ∆gaaC mutant on GA as compared to the reference strain and other GA catabolic pathway deletion mutants. This indicates that 2‐keto‐3‐deoxy‐l‐galactonate is the inducer of genes required for GA utilization.

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Regulation of the lignocellulolytic response in filamentous fungi

Cited by 80 publications

References 103 publications

Network of nutrient-sensing pathways and a conserved kinase cascade integrate osmolarity and carbon sensing in Neurospora crassa

Network of nutrient-sensing pathways and a conserved kinase cascade integrate osmolarity and carbon sensing in Neurospora crassa

Estudo da alteração do perfil de proteínas de fungos filamentosos em diferentes condições de cultivo utilizando ferramentas proteômicas e ensaios enzimáticos

The pathway intermediate 2‐keto‐3‐deoxy‐L‐galactonate mediates the induction of genes involved in D‐galacturonic acid utilization in Aspergillus niger

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