“…There were limitations in the stains that were available to distinguish among these cells at the NMJ and, as argued above for Remak fibers, an inability to resolve them in light microscopy. Now from electron micrographs (Birks et al, 1960a;Heuser and Reese, 1973;Slater, 1968, 1970;Peper et al, 1974;Robertson, 1956), the filling of individual cells with fluorescent dyes using microelectrodes (McMahan et al, 1972), and most recently from immunostaining (Astrow et al, 1994(Astrow et al, , 1998Reynolds and Woolf, 1992;Young et al, 2005) and use of GFP transgenes (Kang et al, 2000(Kang et al, , 2007Zuo et al, 2004) (see Fig. 2), it is apparent that NMSCs cover the synapse.…”