Regulation of the Hypoxia-inducible Transcription Factor HIF-1 by Reactive Oxygen Species in Smooth Muscle Cells

Aiba, Rachida S. Bel; Görlach, Agnes

doi:10.1007/978-1-4419-9280-2_22

Cited by 4 publications

(3 citation statements)

References 21 publications

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“…For these reasons, we hypothesized that ROS may activate HIF-1 in Leydig cells; however, when purified Leydig cells were cultured at 21% or 5% oxygen in the presence of 250 mM H 2 O 2 , there was no change in HIF-1a levels compared with cells cultured without exogenous H 2 O 2 . Studies by BelAiba and Gorlach (2003) have demonstrated that 10 to 50 mM H 2 O 2 increased HIF-1a protein in cultured smooth muscle cells, whereas 100 mM H 2 O 2 decreased HIF-1a levels in these cells. Nitric oxide (NO) has also been shown to induce stabilization of HIF-1a under both normoxic and hypoxic conditions in microvascular endothelial cells (Natarajan et al, 2005) and human oral squamous carcinomas (Quintero et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…It has been demonstrated that ROS can regulate HIF‐1 stability and transcriptional activity in normoxic tissues (Pouyssegur and Mechta‐Grigoriou, 2006). For example, in vitro H 2 O 2 has been shown to contribute to a pro‐oxidant state that promotes increases levels of HIF‐1α in pulmonary smooth muscle cells (BelAiba and Gorlach, 2003).…”

Section: Discussionmentioning

confidence: 99%

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Normoxic Expression of Hypoxia‐Inducible Factor 1 in Rat Leydig Cells In Vivo and In Vitro

Palladino

Pirlamarla

McNamara

et al. 2011

Journal of Andrology

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Hypoxia-inducible factors (HIF) are transcription factors that serve essential regulatory roles in cellular and molecular responses to oxygen debt. HIFs are composed of hypoxiadependent a subunits (1a, 2a, 3a) and an oxygen-independent b subunit. Previously we demonstrated that HIF-1, the master regulator of hypoxic responses, is expressed in the adult rat testis. We hypothesized that HIF-1 is involved in regulating responses to oxygen tension in the testis. Goals of this study were to determine if HIF-2a and HIF-3a are expressed in rat testis, identify testis cell types that express HIF-1a, and examine patterns of testicular HIF-1a protein expression under conditions of ischemia and hypoxia in vivo and in vitro. Reverse transcriptase polymerase chain reaction revealed that mRNA for Hif-1a, Hif-2a, and Hif-3a is expressed in the testis. The HIF-1a protein is the predominant subunit in testis. HIF-1a protein was abundant in normoxic testis, and its levels remained unchanged following ischemia created by surgically induced testicular torsion and reperfusion. Immunoblot and immunocytochemical experiments demonstrated that Leydig cells are the major source of HIF-1a in normoxic and hypoxic testes. To examine potential mechanisms of testicular HIF-1 stabilization, nuclear proteins from Leydig cells cultured in 5% or 21% oxygen, or cells cultured with H 2 O 2 , were analyzed by immunoblotting. Levels of HIF1a were significantly diminished in 5% or 21% oxygen cultures compared with freshly isolated cells. Treating Leydig cells with H 2 O 2 as a source of reactive oxygen species did not affect HIF-1a levels. High levels of constitutively expressed HIF-1a in normoxic Leydig cells suggest potentially unique roles for HIF-1 in Leydig cell responsiveness to oxygen.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Normoxic Expression of Hypoxia‐Inducible Factor 1 in Rat Leydig Cells In Vivo and In Vitro

Palladino

Pirlamarla

McNamara

et al. 2011

Journal of Andrology

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“…Induction of hsp70 is a general stress response and hypoxia inducible factors, beside their response to dissolved oxygen, are closely related to the biotransformation pathways (Regoli and Giuliani, 2014). It was shown that PAHs reduced hypoxia pathways and supported the hypothesis that hif-1α can compete with AhR for a shared arnt pool in fish cells, limiting the activation of the AhR pathways (Bel Aiba and Gorlach, 2003;Rytkonen et al, 2008). In general, the significant expression of hif-1 and hsp70 in fish from Eleyele lake compared with reference site, together with the biotransformation responses and estrogen responses (Adeogun et al, 2016a) indicate that the Eleyele lake biota are experiencing a clear biological stress.…”

Section: Biotransformation and Oxidative Stress Responsesmentioning

confidence: 89%

Xenobiotic biotransformation, oxidative stress and obesogenic molecular biomarker responses in Tilapia guineensis from Eleyele Lake, Nigeria

Ibor

Adeogun

Regoli

et al. 2019

Ecotoxicology and Environmental Safety

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Iron Chelation and the Ventilatory Response to Hypoxia

Pokorski

Antosiewicz

Giulio

et al. 2009

Advances in Experimental Medicine and Biology

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Chelation of iron in in vitro carotid body emulates the effects of hypoxia. The role iron plays in in vivo ventilatory responses is unclear. In the current study we addressed this issue by examining the effects of chronic iron chelation on the hypoxic ventilatory response in 9 conscious Wistar rats. Acute responses to 14 and 9% O(2)in N(2) were recorded in the same rat before and then after 7 and 14 days of continuous iron chelation. Iron chelation was carried out with ciclopirox olamine (CPX) in a dose of 20 mg/kg daily, i.p. Ventilation was recorded with whole body plethysmography. We found that the peak hypoxic ventilation (V(E) achieved during 14 and 9% hypoxia was lower by 239.6+/-55.4(SE) and 269.6.2+/-69.2 ml min(-1)kg(-1), respectively, in the rats treated with CPX for 7 days. The decreases were not intensified by a longer duration of iron chelation. CPX failed to alter hypoxic sensitivity, assessed from the gain of peak V(E) with increasing strength of the hypoxic stimulus. In conclusion, we believe we have shown that iron is operational in shaping the hypoxic ventilatory response, but is not liable to be the underlying determinant of the hypoxic chemoreflex.

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Regulation of the Hypoxia-inducible Transcription Factor HIF-1 by Reactive Oxygen Species in Smooth Muscle Cells

Cited by 4 publications

References 21 publications

Normoxic Expression of Hypoxia‐Inducible Factor 1 in Rat Leydig Cells In Vivo and In Vitro

Normoxic Expression of Hypoxia‐Inducible Factor 1 in Rat Leydig Cells In Vivo and In Vitro

Xenobiotic biotransformation, oxidative stress and obesogenic molecular biomarker responses in Tilapia guineensis from Eleyele Lake, Nigeria

Iron Chelation and the Ventilatory Response to Hypoxia

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