Regulation of the H19 imprinting gene expression in human nasopharyngeal carcinoma by methylation

Ng, Aylwin; Tang, Jing; Goh, Christopher; Hui, Kam M.

doi:10.1002/ijc.10926

Cited by 29 publications

(27 citation statements)

References 53 publications

(57 reference statements)

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“…Fabrication of cDNA spotted arrays The fabrication of the cDNA spotted microarray was essentially according to Brown's protocols (Schena et al, 1995;Ng et al, 2003). The cDNA clones employed for spotting were obtained from Incyte, Inc. (Palo Alto, CA), and I.M.A.G.E.…”

Section: Gene Differential Expression Profilementioning

confidence: 99%

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Familial Characteristics of Kidney-Yang Deficiency and Cold Syndrome

Wang

Pan

et al. 2006

Journal of Toxicology and Environmental Health, Part A

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Family investigation is a reliable model to study the effects of both genetic and environmental factors on human health. This article studies kidney-yang deficiency syndrome and cold syndrome through family investigation and cDNA microarray technology, exploring the effects of both genetic and environmental factors on the health of family members. Particularly, these two syndromes were first assessed by the accumulated clinical scores measured by 40-item scoring tables among 15 family members. The family patterns were obtained and the correlation of these two syndromes was determined. Then the gene differential expression profiles among 12 family members were obtained using an 18,816 clones cDNA microarray. The profiles of the patients with typical kidney-yang deficiency syndrome and cold syndrome were compared to those of normal members and 89 differential expression genes were found. Further, only 22 genes were identified as known functions, and most (16 genes) were associated with the regulation of metabolism, temperature feeling, and growth. Therefore, the formation and development of these two syndromes have not only genetic but also environmental factors, including living conditions and lifestyle.

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Section: Gene Differential Expression Profilementioning

confidence: 99%

“…Total RNA was then extracted using Trizol (Invitrogen Life Technologies, Inc., Carlsbad, CA) as previously described (Ng et al, 2003). The RNA samples were further purified using Qiagen Rneasy (Qiagen, Valencia, CA) according to the procedures recommended by manufacturer.…”

Section: Gene Differential Expression Profilementioning

confidence: 99%

Familial Characteristics of Kidney-Yang Deficiency and Cold Syndrome

Wang

Pan

et al. 2006

Journal of Toxicology and Environmental Health, Part A

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“…10 Recent studies have shown that changes in methylation of promoter/enhancer sites are the trigger for H19 expression in some cancers. [24][25][26][27][28] We have shown that H19 is strongly expressed in over 80% of human bladder carcinomas and that its expression decreases with loss of tumour differentiation. This decrease has prognostic value in predicting early tumour recurrence.…”

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confidence: 99%

H19 expression in hepatic metastases from a range of human carcinomas

Fellig

Ariel²,

Ohana³

et al. 2005

J Clin Pathol

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Aims: To investigate the expression of the imprinted oncofetal H19 gene in hepatic metastases derived from a range of human carcinomas and assess its prognostic value with the view of developing a DNA based treatment for such metastases. Methods: Non-radioactive in situ hybridisation for H19 RNA was performed on paraffin wax embedded sections of liver biopsies or partial hepatectomy specimens, taken from 80 patients with hepatic metastases derived from carcinomas from several medical centres in Israel. The degree of expression was graded qualitatively according to the number of cells expressing H19 and the intensity of staining. The medical files were searched for demographic data and survival times before and after diagnosis of hepatic metastases. Results: H19 expression was found in the hepatic metastases of 64 of 80 patients. High expression (higher staining grades) of H19 in the metastases was found in 43 of 80 patients. However, H19 expression status in the hepatic metastases did not correlate with either the length of time to development of metastasis or overall survival. Conclusions: H19 is highly expressed in more than half of hepatic metastases derived from a range of carcinomas. Thus, these metastases may be suitable candidates for H19 DNA based treatment. Further studies are needed to determine whether H19 expression has prognostic value in metastatic liver disease using larger numbers of specific subtypes of primary carcinomas.T he liver is one of the major sites of cancer metastasis. 1The most common primary malignancies producing hepatic metastases are carcinomas originating in the gastrointestinal tract, breast, and lung, in addition to melanoma, but cancer from any site may spread to the liver. 1 This is often an ominous sign, indicating poor prognosis. 1 However, it has been shown that in cases of colon and perhaps even breast carcinoma with a limited number of metastases, partial hepatectomy can provide hope for longterm survival. Other treatment methods for hepatic metastases include hepatic arterial chemotherapy, systemic chemotherapy, chemoembolisation, and several ablative techniques such as ethanol injection, cryoablation, radiofrequency ablation, microwave ablation, interstitial laser photocoagulation, and high intensity focused ultrasound. [3][4][5] All these methods have some, albeit limited, success in extending patient survival or in palliative treatment. [3][4][5][6][7] There are also some promising novel therapeutic modalities based on the molecular biology of metastasis in various phases of development.8 These include: antiangiogenesis agents, metalloprotease inhibitors, immunotherapy, and gene therapy based treatment strategies. ''The aim of our present study was to determine whether H19 is expressed in hepatic metastases from a range of human carcinomas, with a view to establishing the mechanistic basis for developing a DNA based treatment for such metastases'' H19 is an imprinted, maternally expressed oncofetal gene that functions as an RNA molecule. 9 H19 resides on chromosome ...

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“…HuH7, HLE, PLC/PRF/5 and HuH6, clone 5 (HuH6-C5), were obtained from the Japanese Cancer Research Resources Bank. CNE-2 (a nasopharyngeal carcinoma cell line) has been described earlier (19). Human embryonic kidney 293T cells were obtained from Dr. Paula Lam (National Cancer Centre, Singapore).…”

Section: Methodsmentioning

confidence: 99%

Cloning and Identification of Hepatocellular Carcinoma Down-regulated Mitochondrial Carrier Protein, a Novel Liver-specific Uncoupling Protein

Tan

Ooi

et al. 2004

Journal of Biological Chemistry

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We report the identification of a novel cDNA fragment that shows significantly reduced expression in cancerous tissue compared with paired non-cancerous liver tissue in patients with hepatocellular carcinoma (HCC). The full-length transcript of 1733 bp encodes a protein of 308 amino acids that has all the hallmark features of mitochondrial carrier proteins. We designate the novel protein as HDMCP (HCC-down-regulated mitochondrial carrier protein). The HDMCP orthologs in human, mouse, and rat are found to exhibit close similarity in protein sequence and gene organization, as well as exclusive expression in the liver. Moreover, conserved syntenic regions have been demonstrated at the HDMCP gene locus in the human, mouse, and rat genome. Taken Hepatocellular carcinoma (HCC)1 is one of the most frequent neoplasms worldwide. It has been suggested that chronic hepatitis B and C virus infection, dietary aflatoxin, alcohol consumption, and hepatic iron overload are the etiological factors for HCC development. However, the pathogenic mechanisms responsible for HCC are still not well defined.Mitochondrial defects caused by altered expression of respiratory chain subunits and glycolytic enzymes, decreased oxidation of NADH-linked substrates, as well as mitochondrial DNA mutations have been proposed to contribute to the development and progression of various cancer types including HCC (1-5). It has long been recognized that the most well known function of mitochondria is the production of ATP through oxidative phosphorylation. During mitochondrial respiration, the transfer of electrons along the respiratory chain in the inner mitochondrial membrane is coupled to the translocation of protons (H ϩ ) from the mitochondrial matrix into the intermembrane space. This process generates a proton electrochemical gradient across the inner mitochondrial membrane, which is known as the proton-motive force. This proton-motive force is used to drive the protons back into the matrix through F 0 F 1 -ATP synthase resulting in the synthesis of ATP (6, 7) . Alternatively, the proton-motive force can also be dissipated by "proton leak" catalyzed by multiple parameters, such as classical uncouplers of oxidative phosphorylation (8, 9), fatty acids (10), and the uncoupling protein 1 (UCP1) of brown adipose tissue (11, 12). The mitochondrial membrane potential (⌬⌿ m ) arises from the net movement of positive charge across the inner mitochondrial membrane, reflecting the balance between processes that contribute to the generation of the proton gradient and those that consume it (7).In the early 1980s, Chen and co-workers (13-17) discovered that relative to the mitochondria in normal cells, those in cancer cells displayed a greater uptake and retention of rhodamine 123, a ⌬⌿ m -dependent staining dye, suggesting that cancer cells generate higher ⌬⌿ m compared with normal cells. This is in agreement with a later report showing that ⌬⌿ m of carcinoma cells is ϳ60 mM higher than that of control epithelial cells (18). However, to date, no real underst...

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Regulation of the H19 imprinting gene expression in human nasopharyngeal carcinoma by methylation

Cited by 29 publications

References 53 publications

Familial Characteristics of Kidney-Yang Deficiency and Cold Syndrome

Familial Characteristics of Kidney-Yang Deficiency and Cold Syndrome

H19 expression in hepatic metastases from a range of human carcinomas

Cloning and Identification of Hepatocellular Carcinoma Down-regulated Mitochondrial Carrier Protein, a Novel Liver-specific Uncoupling Protein

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