Regulation of the Glutathione S-Transferase P1 Expression in Melanoma Cells

Slonchak, Andrii; Chwieduk, Agata; Rzeszowska-Wolny, Joanna; Obolenskaya, M. Yu.

doi:10.5772/18747

Cited by 3 publications

(4 citation statements)

References 53 publications

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“…The other antioxidant enzymes include glutathione reductase (GR), glutathione Stransferase (GST), peroxiredoxin, thioredoxin and thioredoxinreductase (Andreyev et al, 2005). GR scavenges O 2 •− and • OH non-enzymatically or by serving as an electron donor to certain enzymes involved in the metabolism of ROS (Andreyev et al, 2005, Slonchak andObolens'ka, 2009). GR helps to regenerate GSH via reduction of oxidized glutathione (GSSG) (Slonchak and Obolens'ka, 2009).…”

Section: General Overview Of Oxidative Stressmentioning

confidence: 99%

Oxidative Stress and Diseases

Lushchak¹,

Gospodaryov²

2012

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Section: General Overview Of Oxidative Stressmentioning

confidence: 99%

Oxidative Stress and Diseases

Lushchak¹,

Gospodaryov²

2012

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“…GR scavenges O 2 •− and • OH non-enzymatically or by serving as an electron donor to certain enzymes involved in the metabolism of ROS (Andreyev et al, 2005, Slonchak andObolens'ka, 2009). GR helps to regenerate GSH via reduction of oxidized glutathione (GSSG) (Slonchak and Obolens'ka, 2009). GST comprises a family of multifunctional phase II biotransformation enzymes with a broad spectrum for a variety of substrates including epoxides, carcinogens, mutagens, 4-hydroxy-2-nonenal and malondialdehyde (MDA) (Andreyev et al, 2005, Slonchak andObolens'ka, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…Generally, hypoglycemic drugs are incapable to prevent The other antioxidant enzymes include glutathione reductase (GR), glutathione Stransferase (GST), peroxiredoxin, thioredoxin and thioredoxinreductase (Andreyev et al, 2005). GR scavenges O 2 •− and • OH non-enzymatically or by serving as an electron donor to certain enzymes involved in the metabolism of ROS (Andreyev et al, 2005, Slonchak andObolens'ka, 2009). GR helps to regenerate GSH via reduction of oxidized glutathione (GSSG) (Slonchak and Obolens'ka, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…GR helps to regenerate GSH via reduction of oxidized glutathione (GSSG) (Slonchak and Obolens'ka, 2009). GST comprises a family of multifunctional phase II biotransformation enzymes with a broad spectrum for a variety of substrates including epoxides, carcinogens, mutagens, 4-hydroxy-2-nonenal and malondialdehyde (MDA) (Andreyev et al, 2005, Slonchak andObolens'ka, 2009). It catalyzes the conjugation of many electrophilic compounds with GSH (Andreyev et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Oxidative Stress in Diabetes Mellitus: Is There a Role for Hypoglycemic Drugs and/or Antioxidants?

Erejuwa¹

2012

Oxidative Stress and Diseases

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SKOV-3 and Me45 Cell Response to Cisplatin-Based Chemotherapy: an in Vitro Study

Gonera,

Wawryka,

Sobkowicz

et al. 2014

Fol. Biol.

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We studied malignant melanoma cell line Me45 and human ovarian carcinoma cell line SKOV-3 (resistant to cisplatin, adriamycin and diphtheria toxin), assessing their expression level of p53, HSP70 and glutathione S-transferase GST-π before and after chemotherapy with cisplatin. These proteins may be responsible for the occurrence of chemoresistance in cancer patients. To assess protein expression we used the immunocytochemical Avidin-Biotin-peroxidase Complex (ABC) method. Before application of chemotherapy, proteins p53, HSP70 and GST-π were present in 100 % of the examined melanoma cells. After the treatment, the intensity of the immunocytochemical reaction for p53 increased, whereas the intensity of immunocytochemical staining for HSP70 and GST-π decreased. In SKOV-3 cells, p53 and HSP70 were present in 100 % of the examined cells both prior to chemotherapy and after it. However, the intensity of the immunocytochemical reaction for p53 decreased, while that of HSP70 increased. As regards GST-π, only 5 % of all examined SKOV-3 cells revealed its expression before chemotherapy. Incubation with cisplatin caused an elevation in the number of ovarian cancer cells expressing GST-π up to 50 %. Moreover, the intensity of the immunocytochemical reaction for GST-π significantly increased.

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Regulation of the Glutathione S-Transferase P1 Expression in Melanoma Cells

Cited by 3 publications

References 53 publications

Oxidative Stress and Diseases

Oxidative Stress and Diseases

Oxidative Stress in Diabetes Mellitus: Is There a Role for Hypoglycemic Drugs and/or Antioxidants?

SKOV-3 and Me45 Cell Response to Cisplatin-Based Chemotherapy: an in Vitro Study

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