Regulation of the early growth response-1 binding protein NAB2 in bovine granulosa cells and effect on connective tissue growth factor expression

Han, Peng; Relav, Lauriane; Price, Christopher A.

doi:10.1016/j.mce.2020.111041

Cited by 4 publications

(1 citation statement)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TGF-β in the endometrium has been linked with embryo receptivity and successful implantation in mice and other mammals [ 38 ]. Lastly, NAB2 controls the transcription of EGR1 , which is implicated in bovine ovarian fibrosis [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of quantitative trait loci and associated candidate genes for pregnancy success in Angus–Brahman crossbred heifers

Hoorn,

Zayas,

Rodriguez

et al. 2023

J Animal Sci Biotechnol

View full text Add to dashboard Cite

Background In beef cattle, more than 50% of the energy input to produce a unit of beef is consumed by the female that produced the calf. Development of genomic tools to identify females with high genetic merit for reproductive function could increase the profitability and sustainability of beef production. Results Genome-wide association studies (GWAS) were performed using a single-step genomic best linear unbiased prediction approach on pregnancy outcome traits from a population of Angus–Brahman crossbred heifers. Furthermore, a validation GWAS was performed using data from another farm. Heifers were genotyped with the Bovine GGP F250 array that contains 221,077 SNPs. In the discovery population, heifers were bred in winter breeding seasons involving a single round of timed artificial insemination (AI) followed by natural mating for 3 months. Two phenotypes were analyzed: pregnancy outcome to first-service AI (PAI; n = 1,481) and pregnancy status at the end of the breeding season (PEBS; n = 1,725). The heritability was estimated as 0.149 and 0.122 for PAI and PEBS, respectively. In the PAI model, one quantitative trait locus (QTL), located between 52.3 and 52.5 Mb on BTA7, explained about 3% of the genetic variation, in a region containing a cluster of γ-protocadherin genes and SLC25A2. Other QTLs explaining between 0.5% and 1% of the genetic variation were found on BTA12 and 25. In the PEBS model, a large QTL on BTA7 was synonymous with the QTL for PAI, with minor QTLs located on BTA5, 9, 10, 11, 19, and 20. The validation population for pregnancy status at the end of the breeding season were Angus–Brahman crossbred heifers bred by natural mating. In concordance with the discovery population, the large QTL on BTA7 and QTLs on BTA10 and 12 were identified. Conclusions In summary, QTLs and candidate SNPs identified were associated with pregnancy outcomes in beef heifers, including a large QTL associated with a group of protocadherin genes. Confirmation of these associations with larger populations could lead to the development of genomic predictions of reproductive function in beef cattle. Moreover, additional research is warranted to study the function of candidate genes associated with QTLs.

show abstract

Section: Discussionmentioning

confidence: 99%

Identification of quantitative trait loci and associated candidate genes for pregnancy success in Angus–Brahman crossbred heifers

Hoorn,

Zayas,

Rodriguez

et al. 2023

J Animal Sci Biotechnol

View full text Add to dashboard Cite

show abstract

Bovine models for human ovarian diseases

Roberts

Huang

2022

Progress in Molecular Biology and Translational Science

View full text Add to dashboard Cite

Regulation of dual specificity phosphatases by fibroblast growth factor signaling pathways in bovine granulosa cells

Relav

Price

2021

Reproduction

View full text Add to dashboard Cite

Controling the duration and amplitude of mitogen activated protein kinase (MAPK) signaling is an important element in deciding cell fate. One group of intracellular negative regulators of MAPK activity is a subfamily of the dual specificity phosphatase (DUSP) superfamily, of which up to 16 members have been described in ovarian granulosa cells. Growth factors stimulate proliferation of granulosa cells through MAPK, PKC and AKT pathways, although it is not known which pathways control DUSP expression in these cells. The aim of the present study was to identify which pathways are involved in the regulation of DUSP expression using a well-established serum-free culture system for bovine granulosa cells. Stimulation of cells with FGF2 increased DUSP1, DUSP5 and DUSP6 mRNA abundance in a time and dose-dependent manner, and increased DUSP5 and DUSP6 protein accumulation. None of the other eleven DUSP measured were regulated by FGF2. Pharmacological inhibition of MAPK3/1 signaling decreased FGF2-stimulated DUSP1, DUSP5 and DUSP6 mRNA levels (p < 0.05) whereas inhibition of PKC did not affect the expression of these three DUSPs. Abundance of FGF2-dependent DUSP6 mRNA was reduced by inhibition of PLC or by chelating calcium, but DUSP5 mRNA abundance was not affected. Abundance of basal DUSP1 and DUSP6, but not DUSP5, mRNA was increased by the addition of the calcium ionophore A23187. We conclude that FGF2 stimulation of DUSP5 abundance requires MAPK3/1 whereas DUSP6 mRNA accumulation is dependent on calcium signaling as well as MAPK3/1 activation, suggesting complex regulation of physiologically important DUSPs in the follicle.

show abstract

Regulation of the early growth response-1 binding protein NAB2 in bovine granulosa cells and effect on connective tissue growth factor expression

Cited by 4 publications

References 41 publications

Identification of quantitative trait loci and associated candidate genes for pregnancy success in Angus–Brahman crossbred heifers

Identification of quantitative trait loci and associated candidate genes for pregnancy success in Angus–Brahman crossbred heifers

Bovine models for human ovarian diseases

Regulation of dual specificity phosphatases by fibroblast growth factor signaling pathways in bovine granulosa cells

Contact Info

Product

Resources

About