Regulation of the centrosome cycle

Fujita, Hiroki; Yoshino, Yuki; Chiba, Natsuko

doi:10.1080/23723556.2015.1075643

Cited by 41 publications

(43 citation statements)

References 104 publications

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“…13,17,40 However, many proteins function to control centrosome duplications. 41 Therefore, the 23% false-positive rate in our study may involve disruption of other centrosomal proteins.…”

Section: Discussionmentioning

confidence: 76%

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Increased centrosome number in BRCA‐related breast cancer specimens determined by immunofluorescence analysis

et al. 2018

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BRCA‐related breast carcinoma can be prevented through prophylactic surgery and an intensive follow‐up regimen. However, BRCA genetic tests cannot be routinely performed, and some BRCA mutations could not be defined as deleterious mutations or normal variants. Therefore, an easy functional assay of BRCA will be useful to evaluate BRCA status. As it has been reported that BRCA functions in the regulation of centrosome number, we focused on centrosome number in cancer tissues. Here, 70 breast cancer specimens with known BRCA status were analyzed using immunofluorescence of γ‐tubulin (a marker of centrosome) foci. The number of foci per cell was higher in cases with BRCA mutation compared to wild‐type cases, that is, 1.9 (95% confidence interval [CI], 1.5‐2.3) vs 0.5 (95% CI, 0.2‐0.8) (P < .001). Specifically, foci numbers per cell in BRCA1 and BRCA2 mutation cases were 1.2 (95% CI, 0.6‐1.8) and 2.2 (95% CI, 1.7‐2.6), respectively, both higher than those in wild‐type cases (P = .042 and P < .0001, respectively). The predictive value of γ‐tubulin foci as determined by area under the curve (AUC = 0.86) for BRCA status was superior to BRCAPRO (AUC = 0.69), Myriad Table (AUC = 0.61), and KOHBRA BRCA risk calculator (AUC = 0.65) pretest values. The use of γ‐tubulin foci to predict BRCA status had sensitivity = 83% (19/23), specificity = 89% (42/47), and positive predictive value = 77% (20/26). Thus, γ‐tubulin immunofluorescence, a functional assessment of BRCA, can be used as a new prospective test of BRCA status.

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“…13,17,40 However, many proteins function to control centrosome duplications. 41 Therefore, the 23% false-positive rate in our study may involve disruption of other centrosomal proteins.…”

Section: Discussionmentioning

confidence: 76%

“…Numerous studies have revealed that inhibition of BRCA function causes centrosome aberrations in human breast cell lines, mice, and human breast tissue . However, many proteins function to control centrosome duplications . Therefore, the 23% false‐positive rate in our study may involve disruption of other centrosomal proteins.…”

Section: Discussionmentioning

confidence: 80%

Increased centrosome number in BRCA‐related breast cancer specimens determined by immunofluorescence analysis

et al. 2018

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“…The “copy once, only once” rule is key to the maintenance of genomic stability in eukaryotic cells. In animal cells, the centrosome copy restriction is mainly achieved through the polo kinase PLK4, which regulates the recruitment of SAS6 to the centriole together with CEP192 and CEP152 (Fujita, Yoshino, & Chiba, ). However, homologues for PLK4, CEP192, CEP152, or STIL (a protein partner of SAS6) were not found in apicomplexan genomes (Morlon‐Guyot et al, ), suggesting that the regulation of centriole formation is performed by other proteins and pathways in these parasites.…”

Section: Discussionmentioning

confidence: 99%

A coiled-coil protein is required for coordination of karyokinesis and cytokinesis in Toxoplasma gondii

Courjol

Gissot

2018

Cellular Microbiology

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Toxoplasma gondii is a unicellular eukaryotic pathogen that belongs to the Apicomplexa phylum, which encompasses some of the deadliest pathogens of medical and veterinary importance. The centrosome is key to the organisation and coordination of the cell cycle and division of apicomplexan parasites. The T. gondii centrosome possesses a particular bipartite structure (outer and inner cores). One of the main roles of the centrosome is to ensure proper coordination of karyokinesis. However, how these 2 events are coordinated is still unknown in T. gondii, for which the centrosome components are poorly described. To gain more insights into the biology and the composition of the T. gondii centrosome, we characterised a protein that resides at the interface of the outer and inner core centrosomes. TgCep530 is a large coiled-coil protein with an essential role in the survival of the parasite. Depletion of this protein leads to the accumulation of parasites lacking nuclei and disruption of the normal cell cycle. Lack of TgCep530 results in a discoordination between the nuclear cycle and the budding cycle that yields fully formed parasites without nuclei. TgCep530 has a crucial role in the coordination of karyokinesis and cytokinesis.

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“…The stereotypic structures of the centrioles are precisely replicated once per cell cycle to ensure that these organelles are stably inherited to both daughter cells . Both centrioles, ordered perpendicularly to each other, concomitantly initiate the assembly of a new, “daughter” centriole during S‐phase of the cell cycle.…”

Section: Definition Of the Centrosome: Structure And Function Of Thementioning

confidence: 99%

Loss and Rebirth of the Animal Microtubule Organizing Center: How Maternal Expression of Centrosomal Proteins Cooperates with the Sperm Centriole in Zygotic Centrosome Reformation

2018

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Centrosomes are the main microtubule organizing centers in animal cells. In particular during embryogenesis, they ensure faithful spindle formation and proper cell divisions. As metazoan centrosomes are eliminated during oogenesis, they have to be reassembled upon fertilization. Most metazoans use the sperm centrioles as templates for new centrosome biogenesis while the egg's cytoplasm re-prepares all components for on-going centrosome duplication in rapidly dividing embryonic cells. We discuss our knowledge and the experimental challenges to analyze zygotic centrosome reformation, which requires genetic experiments to enable scrutinizing respective male and female contributions. Male and female knockout animals and mRNA injection to mimic maternal expression of centrosomal proteins could point a way to the systematic molecular dissection of the process. The most recent data suggest that timely expression of centrosome components in oocytes is the key to zygotic centrosome reformation that uses male sperm as coordinators for de novo centrosome production.

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Regulation of the centrosome cycle

Cited by 41 publications

References 104 publications

Increased centrosome number in BRCA‐related breast cancer specimens determined by immunofluorescence analysis

Increased centrosome number in BRCA‐related breast cancer specimens determined by immunofluorescence analysis

A coiled-coil protein is required for coordination of karyokinesis and cytokinesis in Toxoplasma gondii

Loss and Rebirth of the Animal Microtubule Organizing Center: How Maternal Expression of Centrosomal Proteins Cooperates with the Sperm Centriole in Zygotic Centrosome Reformation

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