Regulation of the Binding of the BMP-2 Growth Factor with Collagen by Blood Plasma Fibronectin

Osidak, E. O.; Osidak, M. S.; Sivogrivov, D. E.; Portnaya, T. S.; Грунина, Т. М.; Soboleva, L. A.; Lunin, V. G.; Karyagina, A. S.; Домогатский, С. П.

doi:10.7868/s0555109914020147

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“…Fibronectin was used to promote the slow release of BMP-2 from the collagen hydrogel with HAP/β-TCP filling. Its competitive adhesion leads to the protein's slow release from the collagen-BMP-2 complex [12,15].…”

Section: Introductionmentioning

confidence: 99%

Osteoinductive Moldable and Curable Bone Substitutes Based on Collagen, BMP-2 and Highly Porous Polylactide Granules, or a Mix of HAP/β-TCP

Vasilyev

Кузнецова

Бухарова

et al. 2021

Preprint

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In dentistry, maxillofacial surgery, traumatology, and orthopedics, there is a need to use osteoplastic materials that have not only osteoinductive and osteoconductive properties but are also convenient for use. In the study, compositions based on collagen hydrogel were developed. Polylactide granules (PLA) or a traditional bone graft, a mixture of hydroxyapatite and β-tricalcium phosphate (HAP/β-TCP), were used for gel filling to improve mechanical osteoconductive properties of compositions. The mechanical tests showed that collagen hydrogels filled with 12 wt% highly porous PLA granules (elastic modulus 373 ± 55 kPa) or 35 wt% HAP/β-TCP granules (elastic modulus 451 ± 32 kPa) had optimal manipulative properties. All composite components were cytocompatible. The cell’s viability was above 90%, and the components’ structure facilitated the cell’s surface adhesion. The bone morphogenetic protein-2 (BMP-2) provided osteoinductive composition properties. It was impregnated directly into the collagen hydrogel with the addition of fibronectin or inside porous PLA granules. The implantation of a collagen hydrogel with BMP-2 and PLA granules into a critical-size calvarial defect in rats led to the formation of the most significant volume of bone tissue: 61 ± 15%. It was almost 2.5 times more than in the groups where a collagen-fibronectin hydrogel with a mixture of HAP/β-TCP (25 ± 7%) or a fibronectin-free composition with porous PLA granules impregnated with BMP-2 (23 ± 8%) were used. Subcutaneous implantation of the compositions also showed their high biocompatibility and osteogenic potential in the absence of a bone environment. Thus, the collagen-fibronectin hydrogel with BMP-2 and PLA granules has optimal biocompatibility, osteogenic, and manipulative properties.

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Section: Introductionmentioning

confidence: 99%