Regulation of the basement membrane by epithelia generated forces

Tanner, Kandice

doi:10.1088/1478-3975/9/6/065003

Cited by 19 publications

(18 citation statements)

References 113 publications

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“…ECM composition and structure vary with developmental stage, tissue and disease [48,49] . The basement membrane (BM) or basal lamina is a specific ECM-type meshwork produced by epithelial and stromal cells, and it plays a crucial role in carcinogenesis and metastasis [50,51] . BM chemical structure includes…”

Section: Lymphocytesmentioning

confidence: 99%

Natural history of hepatic metastases from colorectal cancer - pathobiological pathways with clinical significance

Paschos

Majeed

Bird

2014

WJG

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Colorectal cancer hepatic metastases represent the final stage of a multi-step biological process. This process starts with a series of mutations in colonic epithelial cells, continues with their detachment from the large intestine, dissemination through the blood and/or lymphatic circulation, attachment to the hepatic sinusoids and interactions with the sinusoidal cells, such as sinusoidal endothelial cells, Kupffer cells, stellate cells and pit cells. The metastatic sequence terminates with colorectal cancer cell invasion, adaptation and colonisation of the hepatic parenchyma. All these events, termed the colorectal cancer invasion-metastasis cascade, include multiple molecular pathways, intercellular interactions and expression of a plethora of chemokines and growth factors, and adhesion molecules, such as the selectins, the integrins or the cadherins, as well as enzymes including matrix metalloproteinases. This review aims to present recent advances that provide insights into these cell-biological events and emphasizes those that may be amenable to therapeutic targeting. Paschos KA, Majeed AW, Bird NC. Natural history of hepatic metastases from colorectal cancer -pathobiological pathways with clinical significance.

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Section: Lymphocytesmentioning

confidence: 99%

Natural history of hepatic metastases from colorectal cancer - pathobiological pathways with clinical significance

Paschos

Majeed

Bird

2014

WJG

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“…We are not sure why these authors ascribed the term "benign tumors" to lesions that are not clinically manifested because that was their primary criterion for defining IELs. According to our (and others') understanding of IELs, in addition to the continuity of the basal membrane [10,15,68], they should not exhibit any clinical signs.…”

Section: Discussionmentioning

confidence: 98%

Untitled

2017

Int J Pathol Clin Res

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“…http://dx.doi.org/10.1101/232066 doi: bioRxiv preprint first posted online Dec. 10, 2017; used collagen hydrogels form with fibers that are randomly oriented unless some external micropatterning is imposed during their polymerization (38). Similarly, laminin rich ECMs (Matrigel) form amorphous gels devoid of cell-scale structures (39). To address the need for reproducible 3D culture systems with well-defined matrix architecture and ECM protein composition, we recently developed a method whereby functionalized paramagnetic nanoparticles are magnetically aligned in 3D hydrogels to create fibrils that span microns in length and 10s of nms in widths (17,40).…”

Section: Cc-by-nc-nd 40 International License Peer-reviewed) Is the mentioning

confidence: 99%

Probing cellular response to topography in three dimensions

Paul

Hruska

Staunton

et al. 2017

Preprint

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Biophysical aspects of in vivo tissue microenvironments include microscale mechanical properties, fibrillar alignment, architecture or topography of the extracellular matrix (ECM), and the repertoire of ECM ligands present, all of which provide cues to drive cellular response. Cell-ECM interactions are important regulators of both normal tissue homeostasis and malignancy.Thus, understanding both extracellular cues and the cellular responses they elicit is fundamental to developing therapeutic strategies. Various in vitro platforms for 3D cell culture and tissue engineering have been used to study cellular response to the microenvironment. However, recapitulating the diversity of tissue architectures present in vivo in a controlled manner in threedimensional tissue mimetics is challenging using naturally derived ECM hydrogels. Here, we use a bottom-up approach to build fibrillar architecture into 3D amorphous hydrogels using selfassembly of magnetic colloidal particles functionalized with human ECM proteins. Human ECM proteins associated with organ-specific pathological states were used. We determined that, while the bulk tissue mechanics of hydrogels containing either aligned fibers or randomly distributed colloidal particles were similar, aligned hydrogels exhibited spatial heterogeneities in microscale mechanical properties near aligned fibers. We then used this platform in combination with 2D substrates of defined elastic modulus to decouple the role of topography from microscale tissue mechanics for normal and tumor cells. We determined that topographical cues dominate cellular response for human and normal cells, which responded independently of microscale mechanics and ECM composition in 3D hydrogels. These data suggest that topography alone can drive fundamental cellular responses such as polarization and migration for both normal and transformed cells.

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Regulation of the basement membrane by epithelia generated forces

Cited by 19 publications

References 113 publications

Natural history of hepatic metastases from colorectal cancer - pathobiological pathways with clinical significance

Natural history of hepatic metastases from colorectal cancer - pathobiological pathways with clinical significance

Untitled

Probing cellular response to topography in three dimensions

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