Regulation of the Association of α6β4 with Vimentin Intermediate Filaments in Endothelial Cells

Homan, Suzanne M.; Martinez, Robert J.; Benware, Angela; LaFlamme, Susan E.

doi:10.1006/excr.2002.5643

Cited by 36 publications

(25 citation statements)

References 37 publications

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“…25,33 In simple and stratified epithelia, a 6 b 4 mediates strong adhesion to laminin in the basement membrane due to the unique ability of the b 4 cytoplasmic domain to connect the a 6 b 4 integrin with keratin intermediate filaments. 19 Previous studies from our laboratory demonstrated that the b 4 cytoplasmic domain connects the a 6 b 4 integrin with the vimentin intermediate filament cytoskeleton, suggesting that endothelial a 6 b 4 may also function in mediating strong adhesion, 34,35 which would be consistent with its expression in mature vessels.…”

Section: Discussionmentioning

confidence: 78%

Dynamic Regulation of Integrin α₆β₄During Angiogenesis: Potential Implications for Pathogenic Wound Healing

Desai

Singh

Water

et al. 2013

Advances in Wound Care

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Section: Discussionmentioning

confidence: 78%

Dynamic Regulation of Integrin α₆β₄During Angiogenesis: Potential Implications for Pathogenic Wound Healing

Desai

Singh

Water

et al. 2013

Advances in Wound Care

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“…As mentioned above, recent reports have implicated intermediate filaments as a possible mechanosensor. These filaments are organized at focal adhesions and have been shown to associate with integrin molecules intracellularly [38]. Disruption of intermediate filaments leads to changes in cell shape [39] and cell response to stress [40].…”

Section: Discussionmentioning

confidence: 99%

Modulus-driven differentiation of marrow stromal cells in 3D scaffolds that is independent of myosin-based cytoskeletal tension

Parekh¹,

Chatterjee²,

Lin‐Gibson³

et al. 2011

Biomaterials

116

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Proliferation and differentiation of cells are known to be influenced by the physical properties of the extracellular environment. Previous studies examining biophysics underlying cell response to matrix stiffness utilized a two-dimensional (2D) culture format, which is not representative of the three-dimensional (3D) tissue environment in vivo. We report on the effect of 3D matrix modulus on human bone marrow stromal cell (hBMSC) differentiation. hBMSCs underwent osteogenic differentiation in poly(ethylene glycol) hydrogels of all modulus (300-fold modulus range, from 0.2 kPa to 59 kPa) in the absence of osteogenic differentiation supplements. This osteogenic differentiation was modulus-dependent and was enhanced in stiffer gels. Osteogenesis in these matrices required integrin-protein ligation since osteogenesis was inhibited by soluble Arginine-Glycine-Aspartate-Serine peptide, which blocks integrin receptors. Immunostained images revealed lack of well-defined actin filaments and microtubules in the encapsulated cells. Disruption of mechanosensing elements downstream of integrin-binding that have been identified from 2D culture such as actin filaments, myosin II contraction, and RhoA kinase did not abrogate hBMSC material-driven osteogenic differentiation in 3D. These data show that increased hydrogel modulus enhanced osteogenic differentiation of hBMSCs in 3D scaffolds but that hBMSCs did not use the same mechanosensing pathways that have been identified in 2D culture.

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“…4). This cat paw organization has been used by us as an indicator of hemidesmosome-like protein clusters (25)(26)(27). In contrast, in the majority of cells expressing GFP-␤ 4 K150A and GFP-␤ 4 Q155L, the GFP signal is diffusely distributed along the basal surface of the transfectants.…”

Section: Expression Of ␤ 4 Integrin Subunit Protein Containing Point mentioning

confidence: 99%

Crucial Role of the Specificity-determining Loop of the Integrin β4 Subunit in the Binding of Cells to Laminin-5 and Outside-in Signal Transduction

Tsuruta

Hopkinson

Lane

et al. 2003

Journal of Biological Chemistry

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Regulation of the Association of α6β4 with Vimentin Intermediate Filaments in Endothelial Cells

Cited by 36 publications

References 37 publications

Dynamic Regulation of Integrin α₆β₄During Angiogenesis: Potential Implications for Pathogenic Wound Healing

Dynamic Regulation of Integrin α₆β₄During Angiogenesis: Potential Implications for Pathogenic Wound Healing

Modulus-driven differentiation of marrow stromal cells in 3D scaffolds that is independent of myosin-based cytoskeletal tension

Crucial Role of the Specificity-determining Loop of the Integrin β4 Subunit in the Binding of Cells to Laminin-5 and Outside-in Signal Transduction

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Regulation of the Association of α6β4 with Vimentin Intermediate Filaments in Endothelial Cells

Cited by 36 publications

References 37 publications

Dynamic Regulation of Integrin α6β4During Angiogenesis: Potential Implications for Pathogenic Wound Healing

Dynamic Regulation of Integrin α6β4During Angiogenesis: Potential Implications for Pathogenic Wound Healing

Modulus-driven differentiation of marrow stromal cells in 3D scaffolds that is independent of myosin-based cytoskeletal tension

Crucial Role of the Specificity-determining Loop of the Integrin β4 Subunit in the Binding of Cells to Laminin-5 and Outside-in Signal Transduction

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Resources

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Dynamic Regulation of Integrin α₆β₄During Angiogenesis: Potential Implications for Pathogenic Wound Healing

Dynamic Regulation of Integrin α₆β₄During Angiogenesis: Potential Implications for Pathogenic Wound Healing