Regulation of syrM and nodD3 in Rhizobium meliloti.

Swanson, Jean; Mulligan, John T.; Long, Sharon R.

doi:10.1093/genetics/134.2.435

Cited by 81 publications

(11 citation statements)

References 43 publications

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“…At 30 min after H 2 O 2 addition, cells were harvested as described previously (51). ␤-Glucuronidase (GUS) assays were performed in triplicate as described previously (59).…”

Section: Methodsmentioning

confidence: 99%

“…pAPL10 (57) and pAPL56 were integrated into the S. meliloti genome via single crossover following conjugation by triparental mating to generate katA and oxyR uidA fusion strains in various S. meliloti backgrounds (Table 3). GUS assays were performed in triplicate as described previously (59). pAPL57 was integrated into CL150 via single crossover following conjugation by triparental mating (59), generating oxyR insertion mutant APL97.…”

Section: Methodsmentioning

confidence: 99%

“…GUS assays were performed in triplicate as described previously (59). pAPL57 was integrated into CL150 via single crossover following conjugation by triparental mating (59), generating oxyR insertion mutant APL97.…”

Section: Methodsmentioning

confidence: 99%

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OxyR-Dependent Transcription Response of Sinorhizobium meliloti to Oxidative Stress

Lehman

Long

2018

J Bacteriol

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Reactive oxygen species such as peroxides play an important role in plant development, cell wall maturation, and defense responses. During nodulation with the host plant , cells are exposed to HO in infection threads and developing nodules (R. Santos, D. Hérouart, S. Sigaud, D. Touati, and A. Puppo, Mol Plant Microbe Interact 14:86-89, 2001, https://doi.org/10.1094/MPMI.2001.14.1.86). cells likely also experience oxidative stress, from both internal and external sources, during life in the soil. Here, we present microarray transcription data for wild-type cells compared to a mutant deficient in the key oxidative regulatory protein OxyR, each in response to HO treatment. Several alternative sigma factor genes are upregulated in the response to HO; the stress sigma gene shows OxyR-dependent induction by HO, while expression is induced by HO irrespective of the genotype. The activity of the RpoE2 sigma factor in turn causes increased expression of two more sigma factor genes, and Strains with deletions of showed improved survival in HO as well as increased levels of and total catalase expression. These results imply that Δ strains are primed to deal with oxidative stress. This work presents a global view of gene expression changes, and of regulation of those changes, in response to HO Like all aerobic organisms, the symbiotic nitrogen-fixing bacterium experiences oxidative stress throughout its complex life cycle. This report describes the global transcriptional changes that makes in response to HO and the roles of the OxyR transcriptional regulator and the RpoH1 sigma factor in regulating those changes. By understanding the complex regulatory response of to oxidative stress, we may further understand the role that reactive oxygen species play as both stressors and potential signals during symbiosis.

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“…At 30 min after H 2 O 2 addition, cells were harvested as described previously (51). ␤-Glucuronidase (GUS) assays were performed in triplicate as described previously (59).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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OxyR-Dependent Transcription Response of Sinorhizobium meliloti to Oxidative Stress

Lehman

Long

2018

J Bacteriol

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“…To refine and reduce the minimal pSymA symbiotic genome, we identified symbiotic loci within the 162-kb minimal pSymA of minSymA1.0 based on the literature (13,(20)(21)(22)(23)(24)(25)(26)(27)(28)(29) and then sequentially combined these loci using yeast recombineering to generate a minSymA2.0 genome. Twelve fragments that carried known nod and nif loci from the A117-A118 region of min-SymA1.0 were assembled and combined with three fragments containing the fix-1 locus of A121 (Fig.…”

Section: Refinement Of the Minimal Symbiotic Genome To 75 Kb (Minsyma2)mentioning

confidence: 99%

Minimal gene set from Sinorhizobium ( Ensifer ) meliloti pSymA required for efficient symbiosis with Medicago

Geddes

Kearsley

Huang

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

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Reduction of N2 gas to ammonia in legume root nodules is a key component of sustainable agricultural systems. Root nodules are the result of a symbiosis between leguminous plants and bacteria called rhizobia. Both symbiotic partners play active roles in establishing successful symbiosis and nitrogen fixation: while root nodule development is mostly controlled by the plant, the rhizobia induce nodule formation, invade, and perform N2 fixation once inside the plant cells. Many bacterial genes involved in the rhizobia–legume symbiosis are known, and there is much interest in engineering the symbiosis to include major nonlegume crops such as corn, wheat, and rice. We sought to identify and combine a minimal bacterial gene complement necessary and sufficient for symbiosis. We analyzed a model rhizobium, Sinorhizobium (Ensifer) meliloti, using a background strain in which the 1.35-Mb symbiotic megaplasmid pSymA was removed. Three regions representing 162 kb of pSymA were sufficient to recover a complete N2-fixing symbiosis with alfalfa, and a targeted assembly of this gene complement achieved high levels of symbiotic N2 fixation. The resulting gene set contained just 58 of 1,290 pSymA protein-coding genes. To generate a platform for future synthetic manipulation, the minimal symbiotic genes were reorganized into three discrete nod, nif, and fix modules. These constructs will facilitate directed studies toward expanding the symbiosis to other plant partners. They also enable forward-type approaches to identifying genetic components that may not be essential for symbiosis, but which modulate the rhizobium’s competitiveness for nodulation and the effectiveness of particular rhizobia–plant symbioses.

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“…•Biotin-Produktion in Pflanzen-Gewebe •Tierfutter mit erhöhter Biotinkonzentration Stämme, die gegen verschiedene Biotin-oder Threonin-Analoga (Ohsawa et al, 1989;Kanzaki et al, 1997) (Hoshino et al, 1997) In der vorliegenden Arbeit wurden zum ersten mal umweltisolierte, natürlich nodD3-Promotorkontrolle zusätzlich haben. Bekanntermaßen befinden sich die nodD1 und nodD3-Promotoren unter der Kontrolle von Flavonoiden und Isoflavone (Swanson et al, 1993;Gyorgypal et al, 1991;Dusha et al, 1999). Somit würden die…”

Section: Mikroorganismenunclassified

Umwelt-Genomik als Quelle für die Isolierung von neuen Operons und Genclustern aus mikrobiellen Konsortien

Entcheva¹

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American type cilture colection b Basen BCIP 5-Brom-4-chlor-3-indolyl-phosphat bidest. zweifach destilliert bp Basenpaar(e) BSA Rinderserumalbumin C. Corynebacterium ºC Grad Celsius C-Quelle Kohlenstoff-Quelle ca. circa CS Casamonosäuren cfu Klon bildende Einheiten (engl. colony forming units) Cm Chloramphenicol DIG Digoxigenin DMF Dimethylformamid DMSO Dimethylsulfoxide DNA Desoxyribonukleinsäure DNase Desoxyribonuklease DSMZ Deutsche Sammlung von Mikroorganismen und Zellkulturen DTB Dethiobiotin DTT Dithiothreitol E. Escherichia, Erwinia E-Cup Eppendorf-Reaktionsgefäß EDTA Ethylendiamintetraacetat et al. et alii (und andere) ERG Eppendorf Reaktionsgefäss g Gramm G. Gluconobacter Gram (+) Grampositives Bakterium Gram (-) Gramnegatives Bakterium i. d. R. in der Regel IPTG Isopropyl-ß-d-thiogalactopyranosid K. Klebsiella kb Kilobasen K d Dissoziationskonstante Km Kanamycin l Liter L. Lactobacillus LB Luria-Bertani (ein Nährmedium) M molar MCS Multi-Cloning-Site mg Milligramm µg Mikrogramm min Minute bioA bioD bioB B. flavum C. glutamicum M. leprae bioA bioF bioD C3_227 bioB bioA bioD orf1 orf2 bioF bioB bioH orf3 Kurthia sp. bioC bioHII bioFII A. E. coli S. marcescens S. tiphimurium E. herbicola orf1 bioA bioB bioF bioC bioD bioH bioB bioF bioH bioC bioD bioA M. flagelatum Gram (-) Mikroorganismen bioB bioF bioD bioA bioZ M. loti bioB bioF bioD bioA bioZ A. tumefaciens C58 B.In allen Habitaten wie z.B. Boden, Meerwasser, Biofilmen, Teichen oder Intestinaltrakten, sind mikrobielle Biotin-Produzenten zu finden. Die Biotinbiosynthesegene liegen in der Regel in Operons vor (Serebriiskii et al., 1996; Abb. 4). Biotinbiosynthese ist bereits in Escherichia coli und Bacillus subtilis detailliert untersucht worden (Bower et al., 1996a;Shiuan and Campbell, 1988). An Abb. 4: Biotinbiosynthesegene verschiedener Mikroorganismen. (A) Die Organisation der Biotinbiosynthesegene in verschiedenen Gram-positiven Bakterien; (B) in verschiedenen Gram-negativen Bakterien. Die Pfeile zeigen die Transkriptionsrichtungen der bio-Gene. Abb. 5: Isolierung von Biotin überproduzierenden Mikroorganismen aus Umweltproben/ Umwelt DNA-Bänken. II. Material und Methoden 9 II. Material und Methoden II.1. Organismen, Plasmide und Primer Die in dieser Arbeit verwendeten Bakterienstämme, Plasmide, Cosmide und Primer sind mit ihren relevanten phäno-oder genotypischen Eigenschaften sowie der Herkunft in den Tabellen 1-3 aufgeführt. Tab. 1: In dieser Arbeit verwendete Stämme.

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Regulation of syrM and nodD3 in Rhizobium meliloti.

Cited by 81 publications

References 43 publications

OxyR-Dependent Transcription Response of Sinorhizobium meliloti to Oxidative Stress

OxyR-Dependent Transcription Response of Sinorhizobium meliloti to Oxidative Stress

Minimal gene set from Sinorhizobium ( Ensifer ) meliloti pSymA required for efficient symbiosis with Medicago

Umwelt-Genomik als Quelle für die Isolierung von neuen Operons und Genclustern aus mikrobiellen Konsortien

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