Regulation of storage protein synthesis by nitrogen and sulfur nutrients in alfalfa (Medicago sativa L.) somatic embryos

Lai, Fang-Ming; McKersie, Bryan D.

doi:10.1016/0168-9452(94)90209-7

Cited by 22 publications

(22 citation statements)

References 30 publications

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“…The accumulation of protein at maturation stage is known to be crucial for high vigor and successful conversion of somatic embryos in legumes (21 ). In general, somatic embryos of legumes accumulate relatively low levels of proteins compared to seeds (20,8).…”

Section: Resultsmentioning

confidence: 99%

“…Up to now regeneration protocols are established for them and processes of induction, development, maturation and conversion of somatic embryos are extensively investigated (34,17). Alfalfa plants have been regenerated from callus cultures (5,21 ), suspension cultures (1 0), protoplast cultures (1, 28). However, isolated model genotypes are susceptible to most diseases and lack the vigor of commercial alfalfa cultivars in field environment.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Establishment of Embryogenic Potential of Economically Important Bulgarian Alfalfa Cultivars (Medicago SativaL.)

Barbulova¹,

Iancheva²,

Zhiponova³

et al. 2002

Biotechnology & Biotechnological Equipment

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Establishment of Embryogenic Potential of Economically Important Bulgarian Alfalfa Cultivars (Medicago SativaL.)

Barbulova¹,

Iancheva²,

Zhiponova³

et al. 2002

Biotechnology & Biotechnological Equipment

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“…Glutamine has been reported to play a regulatory as well as a nutritive role in somatic embryogenesis. When included in the medium, glutamine is converted to 5-oxoproline by autoclaving (Lai et al 1992;Lai and McKersie 1994). Other amino acids cannot substitute for glutamine, but 5-oxoproline can mimic the glutamine effect.…”

Section: Role Of Glutaminementioning

confidence: 98%

Glutamine improves shoot morphogenesis in chickpea (Cicer arietinum L.)

et al. 2009

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The use of glutamine has been shown to increase the frequency of organogenesis and regeneration in the in vitro culture of several plants. The effect of glutamine on hormone-induced multiple shoot formation in desi and kabuli genotypes of chickpea (C-235 and PUSA-1053) were evaluated. Embryo axes with or without attached cotyledons were cultured in thidiazuron (TDZ) or 6-benzylaminopurine (BAP)-containing medium, respectively, with various concentrations of glutamine. Glutamine improved and prolonged the multiple shoot regeneration ability of the embryo axis. Chickpea embryo axis with attached cotyledon and cultured in TDZ-containing medium showed improved and prolonged shoot regeneration with 5 mM glutamine, while embryo axis without cotyledon and cultured in BAP-containing medium showed prolonged regeneration ability in 10 mM glutamine. Glutamine, however, did not serve as a substitute for cotyledon. Desi genotype (C-235) showed better response for multiple shoot formation as compared to the kabuli genotype (PUSA-1053). Glutamine at a concentration of 5 mM also improved root formation in excised in vitro shoots.

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“…Somatic embryos differ from zygotic embryos primarily in the late stages of development and often completely lack a maturation phase (Lai et al 1994b).…”

Section: Protein Content Of Developing Somatic Embryosmentioning

confidence: 99%

Protein patterns associated with Pisum sativum somatic embryogenesis

Griga¹,

Horáček²,

Klenotičová³

2007

Biol. plant.

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Total protein patterns were studied in the course of development of pea somatic embryos using simple protocol of direct regeneration from shoot apical meristems on auxin supplemented medium. Protein content and total protein spectra (SDS-PAGE) of somatic embryos in particular developmental stages were analysed in Pisum sativum, P. arvense, P. elatius and P. jomardi. Expression of seed storage proteins in somatic embryos was compared with their accumulation in zygotic embryos of selected developmental stages. Pea vegetative tissues, namely leaf and root, were used as a negative control not expressing typical seed storage proteins. The biosynthesis and accumulation of seed storage proteins was observed during somatic embryo development (since globular stage), despite of the fact that no special maturation treatment was applied. Major storage proteins typical for pea seed (globulins legumin, vicilin, convicilin and their subunits) were detected in somatic embryos. In general, the biosynthesis of storage proteins in somatic embryos was lower as compared to mature dry seed. However, in some cases the cotyledonary somatic embryos exhibited comparatively high expression of vicilin, convicilin and pea seed lectin, which was even higher than those in immature but morphologically fully developed zygotic embryos. Desiccation treatments did not affect the protein content of somatic embryos. The transfer of desiccated somatic embryos on hormone-free germination medium led to progressive storage protein degradation. The expression of true seed storage proteins may serve as an explicit marker of somatic embryogenesis pathway of regeneration as well as a measure of maturation degree of somatic embryos in pea.

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Regulation of storage protein synthesis by nitrogen and sulfur nutrients in alfalfa (Medicago sativa L.) somatic embryos

Cited by 22 publications

References 30 publications

Establishment of Embryogenic Potential of Economically Important Bulgarian Alfalfa Cultivars (Medicago SativaL.)

Establishment of Embryogenic Potential of Economically Important Bulgarian Alfalfa Cultivars (Medicago SativaL.)

Glutamine improves shoot morphogenesis in chickpea (Cicer arietinum L.)

Protein patterns associated with Pisum sativum somatic embryogenesis

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