Regulation of Steroidogenesis in Isolated Leydig Cells

Rommerts, F. F. G.; Bakker, G.H.; Molenaar, R.; Molen, H. J. van der

doi:10.1007/978-1-4684-4634-0_13

Cited by 2 publications

(2 citation statements)

References 31 publications

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“…The cells were then washed, plated, and maintained at 32°C in 5% CO 2 atmosphere in Dulbecco's modified essential medium (DMEM)/F12 (Sigma Chemical Company, St. Louis, MO) (1:1) containing 10% fetal calf serum (FCS; Gibco, Grand Island, NY). In the first 48 hr, the cell 3b-hydrosteroid dehydrogenase activity was assessed histochemically, 16 and positive cells (Leydig cell contamination) were around 1%. After 4-5 days the cells were subcultured to increase cell yield and harvested when confluent.…”

Section: Human Tpmc Isolation and Culturementioning

confidence: 99%

Integrin Pattern and Effect on Contraction in Cultured Testicular Peritubular Myoid Cells

Magnanti¹,

Gismondi²,

Gandini³

et al. 2001

American J Rep Immunol

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Section: Human Tpmc Isolation and Culturementioning

confidence: 99%

Integrin Pattern and Effect on Contraction in Cultured Testicular Peritubular Myoid Cells

Magnanti¹,

Gismondi²,

Gandini³

et al. 2001

American J Rep Immunol

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“…Contamination with peritubular cells, evaluated by cytochemical detection of alkaline phosphatase activity (7), was negligible (1-2%) and furthermore contam¬ ination with Leydig cells, evaluated by cytochemical detection of 3/3-hydroxy steroid dehydrogenase activity (8), was virtually absent.…”

Section: Sertoli Cell Culturementioning

confidence: 99%

Effect of triiodothyronine administration on estrogen receptor contents in peripuberal Sertoli cells

Panno¹,

Sisci²,

Salerno³

et al. 1996

European Journal of Endocrinology

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The effects of thyroid hormone on androgen metabolism in peripuberal Sertoli cells through the inhibition of estradiol production have been reported previously. It was our intention to investigate further the possible role of thyroid hormone on the interaction between testicular steroids and Sertoli cells by analyzing the effects of triiodothyronine (T3) on estrogen receptor content in 2-, 3- and 4- week-old euthyroid rats. Triiodothyronine treatment (3 micrograms/100 body wt per day) given during the last week prior to sacrifice resulted in reduced testicular growth in 2-week-old animals. Sertoli cells from all groups were cultured initially under basal conditions for the first 24 h and subsequently in the presence of testosterone and/or T3 for the additional 24 h. The in vitro addition of T3 induced a decrease of estrogen receptors (ERs) in 2- and 3-week-old animals that appeared more pronounced especially in the presence of T3 and testosterone. When T3 was tested in vivo we noticed that the decrease of ER content was even greater in all three groups under the in vitro influence of both T3 and testosterone. In 3-week-old animals a simultaneous assay of ERs in both nuclear and cytoplasmic compartments was performed. The ER concentrations in the nucleus were closely related to those of the cytoplasm. The in vivo administration of T3 was responsible for a greater decrease of ERs in the nucleus than in the cytosol. On the basis of these results, and in agreement with our previous data, we speculate that the effect of T3 in the maturational events of Sertoli cells could involve both estradiol production and ER content.

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Regulation of Steroidogenesis in Isolated Leydig Cells

Cited by 2 publications

References 31 publications

Integrin Pattern and Effect on Contraction in Cultured Testicular Peritubular Myoid Cells

Integrin Pattern and Effect on Contraction in Cultured Testicular Peritubular Myoid Cells

Effect of triiodothyronine administration on estrogen receptor contents in peripuberal Sertoli cells

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